... ThermoFAD, a ThermofluorÒ-adapted flavin ad hoc detection system for protein folding and ligand binding Federico Forneris, Roberto Orru, Daniele Bonivento, Laurent R. Chiarelli and Andrea MatteviDepartment ... folded and denatured state. The mainadvantage of the method is that it allows a large amount of biochemicaldata to be obtained using very small amounts of protein sample and stan-dard laboratory ... agents, high-affinity ligands, protein complex formation, and other factors that canaffect protein stability. This information is obviouslyvery valuable for any biochemical and biophysicalanalysis,...