Báo cáo khoa học: X-ray crystallography and structural stabi

Báo cáo khoa học: X-ray crystallography and structural stability of digestive lysozyme from cow stomach doc

... X-ray crystallography and structural stability of digestive lysozyme from cow stomach Yasuhiro Nonaka 1 , Daisuke Akieda 1 , Tomoyasu Aizawa 1 , ... and house ﬂy lysozyme is 0.79 A ˚ . The backbone structure of BSL2 is closer to that of the vertebrate nondigestive lysozyme than to that of insect digestive lysozyme. pH dependence of the lytic ... (1976)...

Ngày tải lên : 23/03/2014, 04:21

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Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

... a k cat of 7.7 ± 0.9 s )1 , K m of 0.148 ± 0.028 mm and k cat ⁄ K m of 5.2 · 10 4 m )1 Æs )1 toward shikimate, and a k cat of 7.1 ± 0.7 s )1 , K m of 0.182 ± 0.027 mm and k cat ⁄ K m of 3.9 · ... shikimate, k cat of 7.1 s )1 and K m of 0.182 mm toward NADP, k cat of 5.2 s )1 and K m of 2.9 mm toward NAD. The optimum pH of the enzyme activity is between 8.0 and...

Ngày tải lên : 19/02/2014, 05:20

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Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues. It ... structure of conomarphin was similar to those of other conotoxin cDNAs. The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 re...

Ngày tải lên : 18/02/2014, 17:20

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Báo cáo khoa học: Puriﬁcation and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

... require individual diagnosis and therapy, and an understand- ing of the structure of these allergens is essential to the improvement of diagnosis and the design of adequate therapeutic treatment. ... oligosac- charide (68.3% of the total carbohydrate weight) of BG60 from BGP [13]. Thus, this structure is probably a unique feature of, and the predominant component of, t...

Ngày tải lên : 07/03/2014, 12:20

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Báo cáo khoa học: Puriﬁcation and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

... presence of a very strong negative band at 198 nm. The negative band near 220 nm results from overlapping of the bands of b-sheet (215 nm) and a-helix (209 nm and 222 nm) [22]. Analysis of the ... of Fig. 4A, are shown in Fig. 6 and Table 2. From Table 2, the sum of individual amide I¢ intensity and the intensity percent of each peak from human Fig. 3. Activity ana...

Ngày tải lên : 07/03/2014, 15:20

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Báo cáo khoa học: Identiﬁcation and structural characterization of a sialylated lacto-N-neotetraose structure in the lipopolysaccharide of Haemophilus inﬂuenzae pptx

... (1999) Identiﬁcation of a novel sialyltransferase gene from Haemophilus ducreyi and construction of mutants deﬁcient in the production of sialic acid containing glycoforms of the lipooligo- saccharides. ... presence of sialic acid. A combination of molecular genetics, MS and NMR spectroscopy techniques showed that this structural unit extended from the proximal heptose r...

Ngày tải lên : 23/03/2014, 21:21

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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

... was only  2 kb. Structural and phylogenetic analyses of carp IL-10 Alignment of the deduced amino acid sequence of IL-10 with those of mammalian homologues revealed common structural features ... human and pufferfish (torafugu and spotted green pufferfish) IL-10 sequences. Pufferfish and carp IL-10 genes, clustered together and distant from IL-20 and IL- 24, as recentl...

Ngày tải lên : 20/02/2014, 02:21

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Tài liệu Báo cáo khoa học: Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt

... Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai Ken-ichi Suzuki, Takao Ojima and Kiyoyoshi Nishita Laboratory of Biochemistry and Biotechnology, Graduate School of Fisheries ... phosphate (pH 7.0), and an appropriate amount of enzyme at 30 °C. The reducing sugar liberated by hydrolysis of CMC was determined by the method of Nelson and So...

Ngày tải lên : 20/02/2014, 23:20

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Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

... ﬂuorescence intensity as function of time of double- labeled liposomes made from PS (A) or from PC (B) after the addition of MfGl-IIorLPSReatt = 50 s and subsequent addition of LBP (0.2 m M )att = 100 ... were kind gifts of K H. Wiesmu ¨ ller 1 (Tu ¨ bingen, Germany). Bovine brain 3-sn-PS and egg 3-sn-PC were obtained from Sigma (Deisenhofen, Germany). For pre- paration o...

Ngày tải lên : 21/02/2014, 00:20

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Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of ﬂavonol synthase from Citrus unshiu pptx

... evolved from a common ancestral gene, and the essence of most of the conserved amino acids has been further substantiated by site-directed mutagenesis of FHT [7,9] and by documentation of ligand ... initially from Ruta species. Flavonols originate from ﬂavanones, i.e. (2S)-naringenin, by the consecutive action of FHT and FLS (Fig. 1), and both of these enzymes use mo...

Ngày tải lên : 21/02/2014, 03:20

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