Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... intermediate exchange regime. Thus, the configuration at the hemiacetal carbon of galactose may affect the dissociation constants. By contrast, because chemical-shift changes upon the addition of sugars ... the center of the broadened signals shifted to the position of the second signal correspond- ing to the bound state during titration ([sugar] ⁄ [EW29Ch] = 0.5–3)...
Ngày tải lên: 23/03/2014, 04:21
Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf
... volumes of 5 M urea solution and then run on SDS ⁄ PAGE. Lanes a and d, in the absence of both TnC and Ca 2+ ; lanes b and e, in the presence of TnC and the absence of Ca 2+ ; lanes c and f, in the ... corresponding interaction in rabbit TnI–TnC in the absence of divalent cation. Therefore, this interaction potentially participates in both the Ca 2+ -dependent acti...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo khoa học: Mechanistic studies on bovine cytosolic 5¢-nucleotidase II, an enzyme belonging to the HAD superfamily doc
... a stepwise manner, using an i ncreasing concentration of acetonitrile in the elution solution, and loaded directly on the MALDI target by using the dried droplet technique and a-cyano-4-hydroxycinnamic ... emonstrate the nature of the oxidized enzyme and the extreme sensitivity of C175 and C547 to changes in redox conditions. Different data were generated from the MA...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx
... The time course of the production of transfer products and the degradation of 1 was observed by HPLC analysis as shown in Fig. 1B. The maximum production of 9 and 10 was reached at 4 h, and their ... yields (82 and 71%) depending on the acceptor. The positional isomers 5 and 6 were prepared simultaneously by Gal transfer from Galb-oNP to the OH-3¢ and OH-6¢ positions...
Ngày tải lên: 31/03/2014, 07:20
Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt
... f hydrogen bonds of the side c hain of Arg78 for the simulations in Me 2 SO; the agonist forms a significantly higher number of interactions than the antagonist. For the simulations in water, the differences ... role in the Fig. 4. Backbone cross RMSD matrix for the comparison of the various conformers of the five MD simulations of Table 1. The x and y axes cor...
Ngày tải lên: 07/03/2014, 16:20
Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc
... allowed regions of the Rama- chandran plot. An additional 6% of residues were found in the generously allowed region. None of the res- idues of helices 2 and 3 was found in the disallowed regions. Instead, ... additional upper distance constraints. The 3D structure of micelle-associated VpUcyt was cal- culated on the basis of the final set of proton–proton dis-...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... show any activity must therefore be a consequence of the reactivity of the functional group and further support the hypothesis that E52 acts as an initiating base in the reaction. R105 mutations R105H R105 ... stabilize the transition state and hold E52 in the correct position to initiate the catalytic reaction. The transition state of this type of kinases is conside...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx
... turn) folded structure. The conformational features of AII in the solution state (representing the free ligand conformation) were compared with the conformation of the hormone complexed to the high-affinity ... in conferring the structure of AII. X-ray structure of AII bound to the mAb Fab131: comparison with the NMR solution structure The phenomenon of conf...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
... a-helices on one side of the b -sheet and two on the other side. The rmsd of the heavy atoms (Ca, C, and N) of the protein core, when the NMR structures of both conformers are superimposed on the crystal ... recognition are in accord with the hypothesis that the C -domain could be involved in the regulation of translation termination. As Asp359 and Gly...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B–NS3 protease ppt
... presented the dissociation constant of 3 measured by NMR and, as additional proof for bind- ing of 3 to the substrate-binding site, demonstrated changes in cross-peak positions for residues lining the substrate-binding ... in the crystal structure with BPTI [4] and was confirmed by narrow NMR line shapes. The resonances of the complex with 1 were assigned using NS2B–N...
Ngày tải lên: 07/03/2014, 02:20