Báo cáo khoa học: Tyrosine-dependent basolateral targeting of human connexin43–eYFP in Madin–Darby canine kidney cells can be disrupted by the oculodentodigital dysplasia mutation L90V ppt

... on the west- ern blot and by the minimal effect of chaperonin (Fig. 1B). This suggests that the R256T mutation harms the function of the folded protein rather than the ability of the protein ... overex- pressed in E. coli JM109 cells by using the pWT vector (encoding the mature part of the human MCAD gene, pre- ceded by an artificial methionine, under...

Ngày tải lên: 20/02/2014, 02:21

... [33]. The high-affinity DNA-binding site is located in the N-domain of LF [24], and the ATP- binding site in the C-terminal domain [23]. We obtained these fragments by tryptic hydrolysis of LF ... doi:10.1046/j.1432-1033.2003.03715.x apoptosis-inducing agents. These findings suggest that LF of milk and other human epithelial secretions and body fluids may contribute to cell...

Ngày tải lên: 21/02/2014, 00:20

... or the indicated domains of hsMOK2 and the human polypeptide JLP 1–141 were co-transformed into yeast. The specificity of the interaction between bait and prey was determined by estimating the ... A and protein kinase A. These three serine resi- dues are located in the lamin A ⁄ C-binding domain. Interestingly, we were able to demonstrate that the phosphorylation of h...

Ngày tải lên: 07/03/2014, 01:20

... of DS in vitro and in human cancer cells. Mecha- nistic and kinetic analyses indicate that DS reacts with the active site cysteine residue of NAT1, leading to the irreversible inhibition of the ... inhibition of NAT1 We further analysed the inhibition of NAT1 by DS by carrying out time course inhibition of the enzyme at different DS concentrations. As shown i...

Ngày tải lên: 07/03/2014, 02:20

... not detected in cells producing c-hIRP1, i.e. in which the protein remained in the cytosol. However, aggregates could occasionally be visualized in the cytosol of these cells, whether glucose ... that binding of KGD1p to RNA- interacting patches of IRP1, as suggested by the struc- ture of the latter protein [7], triggers the building of electron-dense par...

Ngày tải lên: 07/03/2014, 10:20

... against human trypsin 4 (obtained by enterokin- ase activation of recombinant human trypsinogen 4) and a synthetic fragment of the N-terminal 28-amino acid leader peptide of trypsinogen 4. We used these mAbs ... transiently transfected cells, is leucine. Results Determination of the 5¢-terminal sequence of human trypsinogen 4 mRNA In the original publication reporti...

Ngày tải lên: 07/03/2014, 10:20

... restoration of MnSOD activity in transformed can- cer cells (via transfection of MnSOD cDNA) results in a slowing of tumor growth in mice, as well as alteration of the transformed phenotype of cancer cells ... This may, at least in part, be due to a loss of the interaction between the side chains of Asn73 and Gln143. The X-ray crystal structure of hMnSO...

Ngày tải lên: 07/03/2014, 11:20

... descriptive, naming parts in terms of their function and relation to other parts, and also describing the status of parts and other objects in the sublanguage. Domain specific information can be used ... though the equipment failure messages had about one-half the number of sentences of the patient histories. 236 sen- tences in the medical domain were analy...

Ngày tải lên: 08/03/2014, 18:20

... activity in one or more conotoxin. White circles represent regions in the molecules where there is variability in the residue type and in the case of loop 2 in both the type and number of residues. The ... Asp residues to be in the N-cap position of helices and the tendency for Pro to also be near the capping position. By capping (or initiating) the h...

Ngày tải lên: 19/02/2014, 12:20

... involved in the processing of apicoplast protein leader sequence. Inhibition of any of these activities could contribute to MA interference in the maturation of the parasite. Polypharmacology of MA The ... cysteine protease inhibitors, such as vinyl sulfones, reduce the initial cleavage of globin peptides in the trophozoite vacuole [36,37]. This effect has been e...

Ngày tải lên: 14/02/2014, 14:20