Báo cáo khoa học: Alternative splicing produces an H-protein with better substrate properties for the P-protein of glycine decarboxylase doc

... according to the formula C = KS 0 ⁄ S, where K = 3 (standard devia- tion = 1.36), obtained from a series of 11 reagent blanks, and S is the slope of the standard curve. The relative standard deviation ... solution of slightly excess cop- per (II) chlorate in an ice-water bath. Several drops of chlo- roform were further added, and the resultant solution was kept at 5 °C fo...

Ngày tải lên: 07/03/2014, 05:20

... leading to the expression of novel splicing isoforms and ⁄ or loss of an existing one. This phenomenon can either generate new substrates for evolution or cause genetic disease when aberrant isoforms ... on splicing. A variety of tools are available to us for this purpose and further investigation of these sequence variants will not only further our understanding of the...

Ngày tải lên: 06/03/2014, 09:22

... explain what makes an exon an exon or an intron an intron. The discovery of the basic splice site consensus sequences during the same years [4,5], and later on of enhancer and repres- sor elements, ... recruitment of the splicing factors to the targeted splicing competent cis-elements, thus forcing the machinery to use the nat- ural sites. Dominski and Kole [61...

Ngày tải lên: 06/03/2014, 09:22

... patterns of FOX1 and -2. Analysis of breast and ovarian cancer using an RT- PCR panel of alternative splicing events indicated that one-third of cases of increased exon skipping in cancer were ... NOVA-regu- lated alternative splicing of an exon in the GABA A gene. Analysis of alterations in alternative splicing in the neocortex of wild-type and Nova2 ) ⁄ )...

Ngày tải lên: 06/03/2014, 09:22

... binding proteins Tat and Rev are key regulators for the expression of the other viral genes, for the synthesis of full-length genomic RNA and, ultimately, for the production of progeny virions (reviewed ... medica- tion schedules, the lack of patient compliance, side- effects associated with the drugs, and, ominously, the development of drug-resistant HIV. For...

Ngày tải lên: 06/03/2014, 09:22

... released from granules of leukocytes and are implicated in inflammatory and fibrotic lung diseases. In the present study, the effects of a-defensins on the proliferation and collagen synthesis of lung ... proliferation and collagen synthesis of two other cell lines of lung fi- broblasts (HFL-1 and LL86). However, the mechanism responsible for the a-defensin-induced prolifer...

Ngày tải lên: 18/02/2014, 06:20

... domain, there is strong potential for improving the performance of our method in this domain. The analysis of vari- ance (ANOVA) and Tukey’s honestly significant dif- ferences (HSD) tests on the classification ... where the value of the j-th element d j is set to the total number of occurrences of the unigram or bigram w j in the review d. To find the suitable candi...

Ngày tải lên: 20/02/2014, 04:20

... on the data and eval- uate the precision and recall. We also run the al- gorithms described in Hu and Liu (2004a) on the same data as the baseline. From Table 2, we can see the precision of base- line ... consider another kind of association: the association between features and units of measurement. For example, in the sen- tence “A mobile phone with 8 mega-pixel, n...

Ngày tải lên: 20/02/2014, 09:20

... colony clone were analyzed for the expression of the expected recombinant proteins. Bacterial expression and purification of the recombinant mouse SULTs pGEX-4T-2 harboring either the full-length ... obtained with an autoFLEX II TOF ⁄ TOF (Bruker Daltonics, Billerica, MA, USA), and the data were ana- lyzed by a mascot search against the SwissProt database. Cosedimentation...

Ngày tải lên: 06/03/2014, 22:21

... ESI- MS analysis of the samples at acid pH makes it possible to delimit the Cu species present in the sample. For the analy- sis of the apo-MT forms, obtained from recombinant Zn–MT forms, and of the ... (CeMT1) and MT2 (CeMT2) wild-type and mutant forms The obtained pGEX constructs were transformed into E. coli BL21 cells for the expression of the cloned cDNAs a...

Ngày tải lên: 07/03/2014, 02:20