Báo cáo khoa học: LIN54 is an essential core subunit of the DREAM / LINC complex that binds to the cdc2 promoter in a sequence-specific manner ppt
Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf
... periplasmic but with an N-terminal transmembrane helix anchoring the pro-tein to the membrane. However, our bioinformaticsanalysis of the N-terminal sequences for NirF forPs. aeruginosa and ... panel and of the kanamycin insertion mutant nirF strain(SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom leftpanel and of SBN13 (DnirF) containingpEG276-nirF in the ... of the substrate of NirF in a mutant that lacks NirF; this too is not trivial as the DnirF straindoes not accumulate readily detectable amounts of an intermediate of d1synthesis.Experimental...
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Tài liệu Báo cáo khoa học: Bacitracin is not a specific inhibitor of protein disulfide isomerase pptx
... insulin arereduced by dithiothreitol, causing the aggregation andprecipitation of the B-chain of insulin, resulting in an increase in light scattering that can be monitored byincreased absorbance, ... Escherichia coli DsbA and DsbC,as well as the isolated catalytic a domain of PDI. Both the PDI a domain and DsbA have a catalytic site, with an associated substrate-binding site, but lack an inde-pendent ... removal of EDTAand addition of bacitracin appeared to be independent of each other (Fig. 2B), implying that any metal ionsbound by bacitracin and taken into the reaction arenot causing the inhibition.It...
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Tài liệu Báo cáo khoa học: It is all about resolution Meeting report based upon presentations at the 10th International Global BioMillennium 2006 symposium on molecular cell biology (Tbilisi, Georgia) docx
... proteins), that differ substantially in theirbiochemical properties and intracellular localization.DAP-kinases (DAPk), Ca2+⁄ calmodulin-regulatedand Ser ⁄Thr kinases, activate signaling pathways that lead ... by A. Francis Stewart(Dresden). Silencing methylations are epigeneticallymaintained via methyltransferases that associate withproteins that recognize the methylated epitope andpropagate the ... covers the pathway forelucidating the basic mechanisms that are pivotal fordrug targeting. Degradation of cellular proteins playsmajor roles in a multitude of basic pathways duringcell life and...
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Tài liệu Báo cáo khoa học: Processing, catalytic activity and crystal structures of kumamolisin-As with an engineered active site pptx
... of the equilib-rium to that favoring cleavage. Although the mechanistic details of the cleavage at acidic pH remain to be clarified, a plausible mechanism is that the proto-nated His78 acts as ... crystallographic and modeling studiesrevealed that the sedolisins (sedolisin, kumamolisin,kumamolisin-As, and CLN2) have an overall fold that is very similar to that of subtilisin [4–8]. The activesites ... percentageintensity of the band of the 57-kDa protein in a total of intensities of the 57-kDa, 38-kDa and 19-kDa bands, where the intensity of the 38-kDa protein band was corrected for the amounts...
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Tài liệu Báo cáo khoa học: Diversity and junction residues as hotspots of binding energy in an antibody neutralizing the dengue virus doc
... struc-tural and genomic data that are available on anti-bodies and their genes, and performed a systematicscanning of the CDR3 loops of mAb4E11 by mutagen-esis of their residues into alanine (Ala ... with alkaline phosphatase. The totalconcentration of MalE-E3-H6 in the binding reaction is given along the x axis, and the optical signal A 405 in the indirect ELISA is givenalong the y axis. ... with KDand the maximal value of the signal as fittingparameters [34]. Twelve concentrations were used and each datapoint was perfomed in triplicate.Paratope of an mAb neutralizing the dengue...
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Tài liệu Báo cáo khoa học: Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl docx
... Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductaseStabilization of an apo-protein by GdmClNidhi Shukla1, Anant Narayan Bhatt1, Alessandro ... as protein denaturants. Analysis of the solvent denaturant curves using these denaturants canprovide a measure of the conformational stability of the protein [1,2]. Protein unfolding ⁄ folding ... function of increasing denatu-rant concentration. Fig. 1D shows the effect of an increasing concentration of GdmCl on the tryptophanfluorescence emission kmax of FprA. An initial decrease in tryptophan...
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Báo cáo khoa học: Visfatin is induced by peroxisome proliferator-activated receptor gamma in human macrophages pdf
... experiments, increasing amounts (5, 10, 50, 100 and200-fold excess) of unlabeled visfatin–PPREwt (5¢-CAATACAGGGCAAAGATCATGGAAG-3¢) or visfatin–PPRE-mut (5¢-CAATACAGGAAAAAGAAAATGGAAG-3¢) oli-gonucleotides ... -dependent manner in primaryhuman resting macrophages and in adipose tissue macrophages, but not in adipocytes. The threefold increase of visfatin mRNA was paralleled by an increase of protein expression ... pathways in a DNA-binding independent manner [19]. This trans-repression activity is probably the basisfor the anti -in ammatory properties of PPARc.PPARc is activated by natural or synthetic ligandssuch...
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Báo cáo khoa học: YidC is required for the assembly of the MscL homopentameric pore potx
... significant reduction of the amount of MscL–HA that co-purified with the inner membranes. To confirm that the co-purified MscL–HA is insertedas an integral membrane protein, rather than beingperipherally ... membranepreparations, indicating that the protein is fully inte-grated into the lipid bilayer (Fig. 4A, right panels).This corroborates our results from the SCAM assay,and again suggests that neither ... recently that YidC is involved in assembly of the MalFGK2maltose transport complex [23]. YidCwas not essential for insertion of MalF into the innermembrane, but was essential for its folding and...
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Báo cáo khoa học: Lpx1p is a peroxisomal lipase required for normal peroxisome morphology potx
... 5¢-CCCGGGAATGGAACAGAACAGGTTCAAG-3¢ and 5¢-AGATCTTTACAGTTTTTGTTTAGTCGTTTT-3¢, and introduced intopGEM-T (Promega, Mannheim, Germany). The ORF wasexcised using XmaI and BglII, and introduced into ... GermanyPeroxisomes are ubiquitous eukaryotic organelles that are involved in lipid and antioxidant metabolism.They are versatile and dynamic organelles engaged in the b-oxidation of long and ... chain fattyacids, in a- oxidation, bile acid and ether lipid synthe-sis, and in amino acid and purine metabolism [1].Peroxisomes are a source of reactive oxygen species,and are involved in the...
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Báo cáo khoa học: NANOGP8 is a retrogene expressed in cancers pdf
... OS732 and Nanog orNANOGP8 in MCF-7 and HepG2 using anti-Nanog antibody. The lack of expression of both Nanog and NANOGP8 in human fibro-blasts is also shown. Actin (43 kDa) was used as loading control.J. ... fibroblasts and human carci-noma tissues of the uterine cervix, breast and urinary bladder is shown. NANOGP8 was expressed in all of the human cancer tis-sues that were tested. NANOGP8 was not ... NANOGP8 is a retrogene expressed in cancersJingyu Zhang1,2,*, Xia Wang1,2,*, Meixiang Li1, Jin Han1, Bing Chen1, Bin Wang1and Jianwu Dai11 Laboratory of Molecular and Developmental...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ