Báo cáo khoa học: LIN54 is an essential core subunit of the DREAM / LINC complex that binds to the cdc2 promoter in a sequence-specific manner ppt

... periplasmic but with an N-terminal transmembrane helix anchoring the pro- tein to the membrane. However, our bioinformatics analysis of the N-terminal sequences for NirF for Ps. aeruginosa and ... panel and of the kanamycin insertion mutant nirF strain (SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom left panel and of SBN13 (DnirF)...

Ngày tải lên: 15/02/2014, 01:20

... insulin are reduced by dithiothreitol, causing the aggregation and precipitation of the B-chain of insulin, resulting in an increase in light scattering that can be monitored by increased absorbance, ... Escherichia coli DsbA and DsbC, as well as the isolated catalytic a domain of PDI. Both the PDI a domain and DsbA have a catalytic site, with an associated subst...

Ngày tải lên: 16/02/2014, 14:20

... proteins), that differ substantially in their biochemical properties and intracellular localization. DAP-kinases (DAPk), Ca 2+ ⁄ calmodulin-regulated and Ser ⁄Thr kinases, activate signaling pathways that lead ... by A. Francis Stewart (Dresden). Silencing methylations are epigenetically maintained via methyltransferases that associate with proteins that recognize the methylated...

Ngày tải lên: 19/02/2014, 02:20

... of the equilib- rium to that favoring cleavage. Although the mechanistic details of the cleavage at acidic pH remain to be clarified, a plausible mechanism is that the proto- nated His78 acts as ... crystallographic and modeling studies revealed that the sedolisins (sedolisin, kumamolisin, kumamolisin-As, and CLN2) have an overall fold that is very similar to...

Ngày tải lên: 19/02/2014, 07:20

... struc- tural and genomic data that are available on anti- bodies and their genes, and performed a systematic scanning of the CDR3 loops of mAb4E11 by mutagen- esis of their residues into alanine (Ala ... with alkaline phosphatase. The total concentration of MalE-E3-H6 in the binding reaction is given along the x axis, and the optical signal A 405 in the indirec...

Ngày tải lên: 19/02/2014, 07:20

... Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl Nidhi Shukla 1 , Anant Narayan Bhatt 1 , Alessandro ... as protein denaturants. Analysis of the solvent denaturant curves using these denaturants can provide a measure of the conformational stability of the protein [1,2]. Protein u...

Ngày tải lên: 19/02/2014, 16:20

... experiments, increasing amounts (5, 10, 50, 100 and 200-fold excess) of unlabeled visfatin–PPREwt (5¢-CAAT AC AGGGCAAAGATCATGGAAG-3¢) or visfatin–PPRE- mut (5¢-CAATAC AGGAAAAAGAAAATGGAAG-3¢) oli- gonucleotides ... -dependent manner in primary human resting macrophages and in adipose tissue macrophages, but not in adipocytes. The threefold increase of visfatin mRNA was paralleled...

Ngày tải lên: 06/03/2014, 22:21

... significant reduction of the amount of MscL–HA that co-purified with the inner membranes. To confirm that the co-purified MscL–HA is inserted as an integral membrane protein, rather than being peripherally ... membrane preparations, indicating that the protein is fully inte- grated into the lipid bilayer (Fig. 4A, right panels). This corroborates our results from the...

Ngày tải lên: 07/03/2014, 02:20

... 5¢-CCCGGGAAT GGAACAGAACAGGTTCAAG-3¢ and 5¢-AGATCTTTA CAGTTTTTGTTTAGTCGTTTT-3¢, and introduced into pGEM-T (Promega, Mannheim, Germany). The ORF was excised using XmaI and BglII, and introduced into ... Germany Peroxisomes are ubiquitous eukaryotic organelles that are involved in lipid and antioxidant metabolism. They are versatile and dynamic organelles engaged in the b-oxidation o...

Ngày tải lên: 07/03/2014, 05:20

... OS732 and Nanog or NANOGP8 in MCF-7 and HepG2 using anti-Nanog antibody. The lack of expression of both Nanog and NANOGP8 in human fibro- blasts is also shown. Actin (43 kDa) was used as loading control. J. ... fibroblasts and human carci- noma tissues of the uterine cervix, breast and urinary bladder is shown. NANOGP8 was expressed in all of the human cancer tis- sues...

Ngày tải lên: 07/03/2014, 12:20