... acetyl-CoA intoacetyl-CoA preincubating it with ATP, acetate, MgCl2,acetyl-CoA synthetase and inorganic pyrophosphatase(PPase). Then, we confirmed that treated acetyl-CoAwas no longer antagonist ... dinucleosidepolyphosphates catalyzed by firefly luciferase and several ligases. Pharmacol Ther 87, 91–102.16 Nakamura M, Maki S, Amano Y, Ohkita Y, NiwaK, Hirano T, Ohmiya Y & Niwa H (2005) Fireflyluciferase ... luminescencebecause it acts as a substrate and not as an allostericeffectorHugo Fraga1,2, Diogo Fernandes1,2, Rui Fontes2 and Joaquim C. G. Esteves da Silva11 Departmento de Quı´mica, Faculdade...