Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt

... 2010 The Authors Journal compilation ª 2010 FEBS family of RNA binding proteins [31–33]. Together with the findings obtained in the present study, interaction of VICKZ and poly(A) binding proteins ... specific compo- nent of vegetally localizing RNPs in Xenopus oocytes. Results In an effort to identify novel proteins that are part of the vegetal RNA localiza...

Ngày tải lên: 23/03/2014, 03:20

... to the (ATT) 10 region (located at the 5¢-end of the ATT-template) folding into a hairpin-like structure. The double helix of the hairpin stem, stabilized by the binding of DAPI into the minor-groove ... AÆA minor-groove, in the binding of DAPI [16]. In conclusion, the main cause of the strong inhibitory effect of DAPI on DNA replication in vitro reported...

Ngày tải lên: 16/03/2014, 23:20

... The quantity of actin in the pellet was plotted against the total quantity of actin in the sample. Results Heat denaturation of actin Before starting the investigation of the HSP25–actin inter- action ... analyzed the effect of HSP25 on the polymerization of intact actin and aggregation of heated actin. Interaction of HSP25 with intact actin In the...

Ngày tải lên: 20/02/2014, 23:20

... interaction, either alone, in the absence of divalent metals, or in combination with ATP and Mg 2+ or Mn 2+ . To resolve the inhibitory effect of ATP on the GlnK–TnrA interaction in the absence of divalent ... was titrated to the binding assays in the absence or pres- ence of 2-oxolguatarate. As shown in Fig. 3A, 0.2 mm ATP was sufficient to inhibit 50% of...

Ngày tải lên: 06/03/2014, 00:21

... containing amino acids 98–110 of the flavivirus E protein) may serve as an internal fusion peptide, as it is directly involved in interactions with target membranes during the initial stages of membrane ... char- acterized by localization in the inner leaflet of the bi- layer of the second one. In a previous study [14], it has been suggested that the preferential...

Ngày tải lên: 07/03/2014, 17:20

... 17.6 Fig. 5. Insertion of stefins into DOPC and DOPG monolayers. (A) Kinetic traces of the insertion into DOPG lipid monolayers at initial pressure of 5 mNÆm )1 . The proteins were injected into the sub- phase ... DOPG. The insertion of proteins into the monolayer generated an increase in surface pressure, D p, from the chosen initial pressure, p 0 (Fig. 5A). At p 0 ¼ 5mN...

Ngày tải lên: 07/03/2014, 17:20

... absence of the interacting proteins. rMP interacted with NV in both the experi- ments (Fig. 4A,B). Nature of the MP–NV interaction To determine the concentration dependence and nature of the interaction ... a major interacting domain within the N-terminal 49 amino acids of the SeMV MP. This is in contrast to observations on CPMV MP, in which the C-terminal...

Ngày tải lên: 15/03/2014, 00:20

... the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer. Therefore, a fixed aliquot of the protein was incubated with 1 ng of the indicated ... the nuclear-enriched fraction of proteins. The effi- ciency of labelling of the single oligomers being not homogenous, the ratios of the different bin...

Ngày tải lên: 16/03/2014, 13:20

... area upon binding being small. These thermodynamics indicate that the binding is dominated by hydrophobic interactions, which is in agreement with inclusion complex formation of c-CD. In addition, ... on the tongue nor the binding affinity of GA to the receptor has been determined, the need for c-CD in the mm range would correlate with the binding affinity between GA...

Ngày tải lên: 16/03/2014, 14:20

... quantities of protein utilizing the principle o f protein dye binding. Anal. Biochem. 72, 248–254. 35. Laemmli, U.K. (19 70) Cleavage of structural proteins us ing the assembly of the h ead of b acteriophage ... whether the increase in c-jun mRNA levels could b e correlated w ith the appearance of the factor, rRLjunRP, involved in the f ormation of complex C2, ob...

Ngày tải lên: 16/03/2014, 18:20