Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt

... 2010 The Authors Journal compilation ª 2010 FEBSfamily of RNA binding proteins [31–33]. Together with the ﬁndings obtained in the present study, interaction of VICKZ and poly(A) binding proteins ... speciﬁc compo-nent of vegetally localizing RNPs in Xenopus oocytes. Results In an effort to identify novel proteins that are part of the vegetal RNA localization machinery in Xenopus oocytes, tandem ... stage of the transport processthey join the localization complex. Interestingly, Stau-fen has been found to interact with kinesin in X. laevis oocytes, and might therefore provide the link betweenthe...

Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx

... to the (ATT)10region (located at the 5¢-end of the ATT-template) folding into a hairpin-like structure. The double helix of the hairpin stem, stabilized by the binding of DAPI into the minor-groove ... AÆA minor-groove, in the binding of DAPI[16]. In conclusion, the main cause of the strong inhibitoryeffect of DAPI on DNA replication in vitro reported in thiswork appears to be linked to the ... the limitedcase of six triplet sequences, the speciﬁcity of the observedeﬀects conﬁrms the higher selectivity of DAPI for the minor-groove of the ATT-triplet with respect to all the otherindividual...

Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc

... The quantity of actin in the pellet was plotted against the totalquantity of actin in the sample.ResultsHeat denaturation of actinBefore starting the investigation of the HSP25–actin inter-action ... analyzed the effect of HSP25 on the polymerization of intact actin andaggregation of heated actin. Interaction of HSP25 with intact actin In the ﬁrst series of experiments we analyzed the effect of HSP25 ... mutant on the kinetics of actin polymerization.Samples containing 1–4 lM of actin (10% of pyrene-labeled protein)were incubated for 5 min in the absence (1) or in the presence of 6 lM of HSP25...

Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx

... interaction, either alone, in the absence of divalent metals, or in combination with ATP and Mg2+or Mn2+. To resolve the inhibitoryeffect of ATP on the GlnK–TnrA interaction in the absence of divalent ... wastitrated to the binding assays in the absence or pres-ence of 2-oxolguatarate. As shown in Fig. 3A, 0.2 mmATP was sufﬁcient to inhibit 50% of the GlnK–TnrA interaction. The inhibitory effect of ATP ... fully inhibitory, and2mm Mg2+or Mn2+restored more than 50% of the GlnK–TnrA interaction in the presence of 2 mm ATP.On the other hand, 2-oxoglutarate did not in uence the GlnK–TnrA interaction, ...

Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc

... containing amino acids 98–110 of the ﬂavivirus E protein) may serve as an internalfusion peptide, as it is directly involved in interactions with target membranes during the initial stages of membrane ... char-acterized by localization in the inner leaﬂet of the bi-layer of the second one. In a previous study [14], it hasbeen suggested that the preferential interaction of the synthetic peptides with anionic ... of the interaction of viral fusion proteins with membranes and the mechan-ism by which these proteins accelerate the formation of membrane fusion intermediates are poorly under-stood [2]. In...

Báo cáo khoa học: Interaction of human steﬁn B in the preﬁbrillar oligomeric form with membranes Correlation with cellular toxicity doc

... 17.6Fig. 5. Insertion of steﬁns into DOPC and DOPG monolayers. (A)Kinetic traces of the insertion into DOPG lipid monolayers at initialpressure of 5 mNÆm)1. The proteins were injected into the sub-phase ... DOPG. The insertion of proteins into the monolayer generated anincrease in surface pressure, D p, from the chosen initialpressure, p0(Fig. 5A). At p0¼ 5mNÆm)1, insertion of the proteins ... [15]. In this study, we measured the interaction of steﬁn B with various combinations of phospholipid monolayersand bilayers. Interaction of steﬁn B in the preﬁbrillaraggregated state with model...

Báo cáo khoa học: Interaction of Sesbania mosaic virus movement protein with the coat protein – implications for viral spread Soumya Roy Chowdhury and Handanahal Subbarao Savithri docx

... absence of the interactingproteins. rMP interacted with NV in both the experi-ments (Fig. 4A,B).Nature of the MP–NV interaction To determine the concentration dependence and nature of the interaction ... a major interacting domain within the N-terminal 49 amino acids of the SeMV MP. This is in contrast to observations on CPMV MP, in which the C-terminal domain was shown to be involved in NV recognition ... mutants of MP were performed to determine the domain responsible for the interaction of MP with CPusing ELISA and yeast two-hybrid (Y2H) assays.Deletion of the N-terminal 49 amino acids of the SeMV...

Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx

... the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer. Therefore, a ﬁxed aliquot of the proteinwas incubated with 1 ng of the indicated ... the nuclear-enriched fraction of proteins. The efﬁ-ciency of labelling of the single oligomers being nothomogenous, the ratios of the different binding signalswithin each lane instead of their ... com-patible with the binding of eEF1A in the CRC (whitearrow), the other of apparent molecular mass of about100 kDa compatible with binding to nucleolin (blackarrow). The competition experiments in...

Báo cáo khoa học: Interaction of gymnemic acid with cyclodextrins analyzed by isothermal titration calorimetry, NMR and dynamic light scattering doc

... area upon binding being small. These thermodynamics indicatethat the binding is dominated by hydrophobic interactions, which is in agreement with inclusion complex formation of c-CD. In addition, ... on the tongue nor the bindingafﬁnity of GA to the receptor has been determined, the need for c-CD in the mm range would correlate with the binding afﬁnity between GA and the receptor. The thermodynamic ... p.p.m. in the complex of GA and c-CD, probably origin-ating from the methyl groups of the genin moiety[20]. Taking the thermodynamic parameters into con-sideration, the NMR results indicate the...

Báo cáo khoa học: Interaction of an 40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver pdf

... quantities of protein utilizing the principle o f protein dye binding. Anal. Biochem. 72, 248–254.35. Laemmli, U.K. (19 70) Cleavage of structural proteins us ing the assembly of the h ead of b acteriophage ... whether the increase in c-jun mRNA levels could b ecorrelated w ith the appearance of the factor, rRLjunRP,involved in the f ormation of complex C2, observed with rRNE-d, and if the time of ... sensitivity to minor groove bindingdrugs, actinomycin D and distamycin A, conﬁrms that theseare m inor gr oove binding proteins.rRLjunRP is an  40 kDa protein that interacts with the RLjunRP–Jun-25...

Xem thêm

Từ khóa: Nghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoại Biện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọ Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit ĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN Phối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọ Phát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninh Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ long Nghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chế Nghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000 Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thơ nôm tứ tuyệt trào phúng hồ xuân hương BT Tieng anh 6 UNIT 2 Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘI Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam HIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀM MÔN TRUYỀN THÔNG MARKETING TÍCH HỢP