Báo cáo khoa học: The molecular identity of the mitochondrial Ca2+ sequestration system potx

Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf

... the projection of the tilt of the z axis on the x¢,y¢ plane (deﬁned direction of tilt of z and the x¢ axis), and j % a + c deﬁnes the location of the rhombic (x, y) axes. The orientation of the ... at position E7 as the source of the H-bond to ligand on the basis of apartial sequence, which had indicated a Tyr on the distalE-helix. The similarity in the 1HNMRspectraofthethree globin ... consistent with the % 10° tilt of the Fe-CN vector from the heme normal to avoid van der waalsoverlap with the Tyr32(B10) Og. The location of the rhombic axes, j % 50 ± 10°,isinagreementwiththeexpectations...

Tài liệu Báo cáo khoa học: An autoinhibitory effect of the homothorax domain of Meis2 ppt

... presence of alternative splicingaround the 5¢-end of exon 6 of Meis2 and Meis3 was tested by RT-PCR. The positions of molecular mass markers are shown to the left,and the size in base pairs of the ... However, the activity of the full-length Meis1awas not tested, and on the basis of our work we expectthat its activity would be inhibited by the conservedHth domain. The Meis1a isoform, in which the C-terminal ... independently of Pbx, and does notpossess the autoinhibitory function of the Hth domain.In summary, our data suggest that one function of the conserved Hth domain is to inhibit the activity of the transcriptional...

Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx

... advantage of p53 mutation analysis,and a unique feature of this database, is the availabil-ity of the residual activity of the majority of p53 mis-sense mutants. The biological activity of mutant ... of a severe mutant. At the otherend of the spectrum, where we have low energy, thereis 75% probability for the mutation to be nonsevere if the energy is 0.125 or lower. Thus, on the basis of ... buried inside the protein when a spherewith the size of a water molecule van der Waals’ radius is rolled over the protein surfaceSimilarity of the surroundings* Measure of the percentage of amino...

Tài liệu Báo cáo khoa học: Substrate speciﬁcity of the pseudouridine synthase RluD in Escherichia coli doc

... obtained with the transition at posi-tions 1915 and 1917. These mutations had an effect on the formation of pseudouridine exclusively at the mutant position and did not affect either of the othertwo ... bySouthern analysis. The rluD::cat strain had a full deletion of both rluD and yﬁH, together with their annotated pro-moter sequences. The inserted cat cassette had a distance of 90 nt from the ... at which stage of the complex maturationprocess does the modiﬁcation of a given nucleosideoccur and (b) how does the corresponding enzyme rec-ognize the target site within the tRNA architecture[20]?...

Tài liệu Báo cáo khoa học: Receptor binding characteristics of the endocrine disruptor bisphenol A for the human nuclear estrogen-related receptor c pptx

... Given that the roles of these residues deﬁnitely conﬁrm each other, the dif-ference in their signiﬁcance might be attributable to the importance and ⁄ or necessity of the receipt of the phenol-hydroxyl ... environments of BPA in the ligand-binding pocket of the ERRc. The proximity of each amino acid residue (within adistance of 5 A˚) to BPA is shown in the boxes depicting the a -heli-ces. The portrait ... for the major role of Arg316 in arresting the ligandWhen the amino acid sequences of the LBD of all the NRs were aligned to that of ERRc, it became notice-able that 26 receptors among the total...

Tài liệu Báo cáo khoa học: N-terminal extension of the yeast IA3 aspartic proteinase inhibitor relaxes the strict intrinsic selectivity ppt

... each extremity of the polypeptidechain, must of necessity arise from contacts that aremade outwith the active site of the enzyme. The effect of removal of the N-terminal extension The full N-terminal ... binding of this helical N-terminal seg-ment of IA3. The E + I « EI balance thus lies far to the right and the addition of further residues at the N-terminus or beyond residue 34 of the inhibitorysegment ... residues69–81 from the S. castellii sequence is small comparedto that achieved by location of the full-length extensionat the N-terminal end of the polypeptide. The beneﬁt of these alterations...

Tài liệu Báo cáo khoa học: Salt-induced formation of the A-state of ferricytochrome c – effect of the anion charge on protein structure docx

... less sensitive to the pres-ence of the anion. These residues also modulate the conformation of unfol-ded cytochrome c, inﬂuencing its spin state and the coordination to the prosthetic group.AbbreviationCT, ... effect of Cl–reﬂects the different afﬁnities of the two anionsfor the protein [11,17].We also monitored the effect of sulfate on the perchlorate-induced A-state. As shown in Fig. 6, addi-tion of ... that the variants undergo very fast optical (and thus structural)changes within the dead time of the stopped-ﬂowapparatus. In the case of the K13N mutant, this hypo-thesis ﬁnds support from the...

Tài liệu Báo cáo khoa học: Co-operative effect of the isoforms of type III antifreeze protein expressed in Notched-ﬁn eelpout, Zoarces elongatus Kner ppt

... on, the addition of a smallamount of a QAE1 isoform, nfeAFP8 (Fig. 7). This issimilar to the case of the QAE2 isoform, nfeAFP13; the activity of its monomer was enhanced by the pres-ence of ... sequence alignment of nfeAFPs The complete amino acid sequences of the 13 isoforms of nfeAFP were determined based on the cDNAABFig. 2. (A) HPLC puriﬁcation of the isoforms of type III AFP from ... in Fig. 4. The sequence identity within the 13 isoforms is  48%. The identity is 77% when compared within the SP iso-forms, 76% within the QAE1 isoforms, and 91%within the QAE2 isoforms, respectively....

Tài liệu Báo cáo khoa học: A kinetic model of the branch-point between the methionine and threonine biosynthesis pathways in Arabidopsis thaliana doc

... interms of the number of the enzymes involved, the kineticmechanisms of the competing enzymes and the number aswell as the nature of the allosteric controls. Whether suchdiversity in the organization ... changes in the concentration of Pido notmodify the partition (Fig. 3A). However, the presence of Piconsiderably affects the dynamics of the system. Indeed, in the presence of 10 mMPi, the model ... was of special interest to use the in vitro system to test the effect of AMP on the partition of the ﬂux of Phser underphysiological conditions. The partition was measured in the conditions of...

Tài liệu Báo cáo khoa học: "On Learning Subtypes of the Part-Whole Relation: Do Not Mix your Seeds" pdf

... extraction (IE) harder whenlearning the individual types of part-wholerelations? That is, we determine whether the performance of state -of -the- art IE systems inlearning the individual part-whole relationtypes ... were discarded. The precision of the tuples discovered by the different seed-sets in the last iteration of our algorithm are in Table 3.These results reveal that the precision of har-vested ... comparing the results of these three sets among themselves, andwith the results of the member -of and contained-in seeds, indicating insigniﬁcant similarity andoverlap. Examining the patterns...

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