Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx

... 237 amino acids 15 amino acids ⁄ 14 amino acids 25 kDa ⁄ 4.07 PaTry2 GU338028 870 798 nucleotides 58 nucleotides AATAAA ⁄ 10 nucleotides 266 amino acids ⁄ 237 amino acids 15 amino acids ⁄ 14 amino ... nucleotides ATTAAA ⁄ 13 nucleotides 278 amino acids ⁄ 249 amino acids 15 amino acids ⁄ 14 amino acids 27 kDa ⁄ 4.6 New members of the brachyurins family in l...

Ngày tải lên: 23/03/2014, 03:20

... monitoring the change in the concentration of p-nitroaniline. The increase in the rate of isomerization is implicit in the increased rate of p-nitro- aniline release, because catalysis of isomerization ... cellular contents of the proteins that are associated with adaptation at these conditions usually increase. More specifically, the cellular content of a p...

Ngày tải lên: 16/03/2014, 16:20

... differentiate the chirality of 2-phenylbutanoic acid and the ee value of unreacted acid was almost the same as the case of 2-phenylpropanoic acid. In the case of 2-hydroxymethylpropanoic acid (tropic acid) , which ... ketoprofenyl- CoA catalyzed by LUC-H was examined to obtain the detailed reaction information. The efficiency of thioest- er formation was calcu...

Ngày tải lên: 07/03/2014, 10:20

... change of the Fe(II) spin state and no replacement of any of the two axial ligands of the iron, His18 or RNO, by an amino acid side-chain of the antibody protein. Binding site topology of antibody ... the binding of a ligand, such as nitrosoalcane, to the iron of MP8, it brings a partial steric hindrance on the distal face of MP8 and thus controls acces...

Ngày tải lên: 19/02/2014, 12:20

... indolepyruvate decarboxylase from Enterobacter cloacae , an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid Anja Schu¨tz 1 , Tatyana Sandalova 2 , Stefano Ricagno 2 , Gerhard ... decarboxylase, suggesting that the interactions with the cofactor thiamin diphosphate and the catalytic mechanisms are very similar. The substrate binding site in...

Ngày tải lên: 20/02/2014, 11:20

... yields information on the chemical nature of the individual radicals of the radi- cal pair state, and their interaction with each other and with their immediate surroundings. EPR ⁄ ENDOR investigations ... spectrum of the flavin radical of a protein-bound FMN radical of the LOV1 domain (C57M mutant) of Chlamydomonas reinhardtii phototropin [34]. Experimental and cal...

Ngày tải lên: 16/03/2014, 02:20

... (peroxisome) LAAO (snake) 19 4.0 L -amino acids amino acids metabolism (venom toxin?) GOX (bacterial) 14 4.0 glycine, sarcosine amino acids metabolism MSOX (bacterial) 10 4.3 sarcosine amino acids metabolism Flavin-dependent ... with other flavin amine oxidases, including the overall fold of the amine oxidase domain region and details in the active site that are relevant for...

Ngày tải lên: 16/03/2014, 02:20

... state governs the association of Yap4p and Lot6p. Although it appears likely that the redox state of the FAD cofactor of NQO1 and NQO2 plays a similar role in the mammalian system, unequivocal ... acid side chains (Fig. 2) [68]. As mentioned in the introduc- tion, QRs also adopt a flavodoxin-fold and the isoal- loxazine ring engages in a similar interaction wit...

Ngày tải lên: 16/03/2014, 02:20

... CAGACTAGTGGTTAGAGGAGA GGAATGATGCTGTAGAGACA DENV-1 92.1 ± 0.57 73.6 ± 2.31 1G4P217 ATATGCTGAAACGCGTGAG CATCATGAGACAGAGCGAT DENV-3 90.3 ± 1.09 83.9 ± 5.16 1G5P30 TTCCAACAAGCAGAACAACAT GCTACAGGCAGCACGGTTT DENV-4 ... Group 4 ATATGCTGAAACGCGTGAG CATCATGAGACAGAGCGAT DENV-3 104 382 279 1G5P30 Group 5 TTCCAACAAGCAGAACAACAT GCTACAGGCAGCACGGTTT DENV-4 9903 10318 415 C. Gijavanekar et al. PCR detection...

Ngày tải lên: 14/02/2014, 19:20

... (Osaka, Japan). BM CGTase was obtained from Amano Enzyme Inc. (Aichi, Japan) and had a specific activity of 1003 UÆmg )1 of dextrinizing activity [13]. A1 1 CGTase was purified using starch adsorption ... resulting from an increase in the turnover rate (k cat ) and the binding affinity (K m ) (Table 3). The K m values of the CLIP CGTases in the coupling reaction indicate...

Ngày tải lên: 07/03/2014, 10:20