... change in the conformation of this loop. Changes in the conformation of the backbone at this point and changes in the orientation of other side chains, as aresult of the introduction of the large ... the structure of these pro-teins. In the peptide bond region, there were no signi - cant differences between wild-type enolase and the variants, indicating that the variants were folded cor-rectly. ... markers of 97, 66, 45, 31,21 and 14 kDa. Lanes 2 and 3, G157D. Lanes 4 and 5, G376E.Lanes 6, 7 and 8, wild-type.Table 2. Masses of fragments formed by cleavage of the G376Evariant of yeast...