Báo cáo khoa học: Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA docx

... FEBS Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA Mauricio B. Goldfeder and Carla C. Oliveira Department of Biochemistry, ... 5¢-GGATCCATGGGC AAACGCGGGAGCC-3¢ and 5¢-ATCGATGTCGACTCA TTTTTCTCCAGTAATGAAGAG-3¢. The gene was cloned in fusion with yEGFP3 using BamHI...

Ngày tải lên: 22/03/2014, 21:21

... SM, Dalton JP & Ranganathan S (2009) An integrated transcriptomic and proteomic analysis of the secretome of the helminth pathogen, Fasciola hepatica: proteins associated with invasion and ... shows the alignment of FH8 with the N-terminal and C-terminal fragments of CaMs and also with calbindin D 9k . The C-terminal domain of TnC is also shown, because...

Ngày tải lên: 15/03/2014, 00:20

... available in machine-readable form. We have obtained and converted these materials by special arrangement. The materials contain high-quality literal translation. Statements in LegCo may ... be made using either English or Cantonese, and are tran- scribed in the original language. A translation to the other language is made later to yield com- plete parallel texts, with an...

Ngày tải lên: 17/03/2014, 09:20

... in phase, such as biomass concentration, content of storage carbohydrates, and ethanol and acetate production. The molecular basis of the relationship of these oscillations to the cell cycle ... strain in a batch reactor started after a lag phase of % 30 h with a maximal growth rate (l m ) close to 0.07 h )1 . The respiratory quotient (RQ) of % 1 and the abse...

Ngày tải lên: 30/03/2014, 16:20

... His-tag and 22 kDa) appeared when probing the recombinant protein and a trophozoite extract of P. falciparum.This result, and the fact that PfPlrx was amplified from a cDNA library, indicate that the ... characteristics. In the mal- arial parasite, Plasmodium falciparum, a functional thio- redoxin and glutathione system have been demonstrated and are considered to be...

Ngày tải lên: 31/03/2014, 07:20

... were applied on a Formvar and carbon coated grid. After 5 min the sample was soaked away and stained with 1% (v ⁄ v) uranyl acetate. Samples were observed with a Philips (Amsterdam, the Netherlands) ... ste- fin B and the P79S mutant of the variant at both pH 7 and pH 5. Each panel is a plot of heat change on ligand addition (kcalÆmole )1 ) against the ligand ⁄...

Ngày tải lên: 19/02/2014, 06:20

... to the succeeding n − 1 words plus the current word. We train a backward n-gram language model on back- ward n-grams and integrate the forward and back- ward language models together into the ... from training data to enhance conventional n-gram lan- guage models and extend their ability to capture richer contexts and long-distance dependencies. In particular, we integrate b...

Ngày tải lên: 07/03/2014, 22:20

... Tsubouchi H, Naka D, Takahashi K, Okigaki M, Arakaki N, Nakayama H, Hirono S, Sakiy- ama O, Takahashi K et al. (1989) Molecular cloning and sequence analysis of cDNA for human hepatocyte growth factor. ... Yokohama, Japan 2 Advanced Medical Research Laboratory, Mitsubishi Tanabe Pharma Corporation, Kamoshida-cho, Aoba-ku, Yokohama, Japan Introduction Type II transmembrane serine proteases...

Ngày tải lên: 15/02/2014, 01:20

... of 1% FDAA (Sigma- Aldrich, Munich, Germany) in acetone were added and the solution was heated at 37 °C for 1 h. The derivatization reaction was terminated by the addition of 20 lLof1m HCl. After ... Marburg, Germany Introduction Bacterial growth is strongly influenced by the availabil- ity of iron as an essential trace element employed as a cofactor [1]. The fact that the...

Ngày tải lên: 16/02/2014, 09:20

... procedure The assay was run as a three-step assay: initial incubation of the sample and probe, addition and incubation of the sample and acceptor beads in the plate wells, and addition of donor beads with ... that the assay specifically measures HSF1 DNA-binding activity. Analytical range and precision The analytical range of the assay was evaluated using kn...

Ngày tải lên: 18/02/2014, 14:20