Báo cáo khoa học: Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes potx

... plant calcium-dependent protein kinases resembles that of animal protein kinases C, in this study, we used the Xenopus laevis oocyte protein kinase C to identify the target sites of calcium-dependent ... mutant that was insensitive to protein kinase C. These results indicate that, in plant cells, a kinase analogous to protein kinase C might exist that m...

Ngày tải lên: 22/03/2014, 21:21

... rearrangement, the change in the electric field and steric hindrance from a phosphate group can have biologically significant Fig. 6. Electron microscopy images of in vitro filaments of peptide R4 (left) and phosphopeptide ... hampered by the low specificity of protein kinases and the highly dynamic turnover of phosphorylation in vivo. Site-directed mutagenesis, which co...

Ngày tải lên: 16/03/2014, 05:20

... 5¢-AAGGATCCGTTCACCATGTCTATTCTCAAGATCCATG-3¢ AS: 5¢-CACAGCGGCCGCTTAAGCGTAATCTGGAACATCG-3¢ MBP-1 ⁄ FL S: 5¢-CCCAAGCTTAACTAAAGAAAAGTTTCCCCATCTCCC -3¢ AS: 5¢-CTCATGGGTCACTGAGGCTTTTTATTTTG-3¢ MBP-1 ⁄ s-UTR S: 5¢-CCCAAGCTTTATTCAAATTGACTTAGTGTGTGTG-3¢ AS: ... 5¢-CCCGGATCCATGGATTACAAGGATGACGACGATAAGATGCAAATCTTCGTGAAGACCCTGACTG-3¢ AS: 5¢-TTAACCCCCCCTCAAGCGCAGGACCAAG-3¢ Mutagenesis ENO1-mut ⁄ FL S: 5¢-...

Ngày tải lên: 29/03/2014, 21:20

... Hinnebusch AG (1998) Dimerization by translation initiation factor 2 kinase GCN2 is mediated by interactions in the C- terminal ribosome-binding region and the protein kinase domain. Mol Cell Biol ... (left panel). (B) HeLa cells mock-transfected (–) or transfected using increasing amounts (0–10 lg) of Nck-1 cDNA containing plasmid were starved of amino acids for 10 min....

Ngày tải lên: 07/03/2014, 05:20

... to the manufacturer’s protocol. Other analytical procedures Protein concentrations were estimated using a bicinchoninic acid protein assay kit using bovine serum albumin as standard, according ... results in repression of the bacterial cross-linking activities by preventing the activa- tion of TGase. Investigations were therefore carried out to examine the in uence of...

Ngày tải lên: 31/03/2014, 07:20

... entrapped in the residual pellet which is not solubilized by urea. Effect of dopamine on MPTP and MPP + induced changes in kinetics of the aggregation of a-synuclein a-Synuclein was incubated in the ... understand the change in sur- face morphology of the protein following aggregation. Monomeric a-synuclein showed the presence of small particles corresponding...

Ngày tải lên: 14/02/2014, 19:20

... (2001) Assembly of scaffold-mediated complexes containing Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phos- phorylation of Cdc24p. J Biol Chem 276, ... beads and incubated with a yeast lysate of ARE1-9myc, ARE2-9myc or CYC8-9myc cells. Eluted proteins were analyzed by immunoblotting using anti-myc IgG. One per cent of the input...

Ngày tải lên: 18/02/2014, 13:20

... participates in an intricate network of interactions that involve other amino acids and the prosthetic group, contributing to the correct position- ing of FAD and the substrate NADP + [12]. The other tyrosine ... k cat reduction of more than 99% and a 20-fold increase in K m . The introduced changes resulted in a 1300- and 2200-fold decrease in the catalytic efficie...

Ngày tải lên: 18/02/2014, 17:20

... released from MTs than cadmium, which is probably a combination of tighter binding of cadmium than zinc in metallothioneins and the preference of zinc in the more flexible b-domain. In addition to the already ... of MTs in NO-induced changes in intra- cellular zinc homeostasis has been reported by St Croix et al.[30]. Correspondence to K. Zangger, Institute of Chemi...

Ngày tải lên: 07/03/2014, 15:20

... observe an early significant increase in endogenous AEA levels in the ischemic striatum but not in the cortex of rats subjected to MCAo. The lack of acute changes in endocannabinoid levels in the cortical ... FEBS The lack of change in CB1 receptor binding capacity following MCAo was also confirmed by data showing that CB1 receptor striatal content was not modifie...

Ngày tải lên: 23/03/2014, 07:20