... (2001) Assembly of scaffold-mediated complexescontaining Cdc42p, the exchange factor Cdc24p, and the effector Cla4p required for cell cycle-regulated phos-phorylation of Cdc24p. J Biol Chem 276, ... beadsand incubated with a yeast lysate of ARE1-9myc, ARE2-9myc orCYC8-9myc cells. Eluted proteins were analyzed by immunoblottingusing anti-myc IgG. One per cent of the input is shown. Predictedmolecular ... The dele-tion of either ERG4 or NCP1 in the cla4D backgroundis lethal, indicating that CLA4 and these genes, encod-ing proteins involved in sterol synthesis, may function in the same pathway(s)...