Báo cáo khoa học: Selection of full-length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence-activated cell sorting screening doc

... Authors Journal compilation ª 2010 FEBS 2303 Selection of full-length IgGs by tandem display on filamentous phage particles and Escherichia coli fluorescence-activated cell sorting screening Yariv Mazor 1,2 , ... permitting the selection of full-length IgGs from libraries displayed on phage. We believe that sequential selection by phage...

Ngày tải lên: 22/03/2014, 21:21

... attentional failures after they had been working for more than 16 hours on the traditional schedule [2]. Letter Effect of intern’s consecutive work hours on safety, medical education and professionalism Christopher ... hours or more consecutive work are unsafe. Efforts to reduce work hours should focus first and foremost not on the frequency of extended-duration work shifts, but...

Ngày tải lên: 25/10/2012, 10:45

... in HeLa cells. (A) Cleavage of PARP by benomyl treatment. HeLa cells were treated with the indi- cated concentrations of drugs for 40 h and equal amounts of cell lysates were resolved by SDS ⁄ ... ratio of the width to the height of the chromosomal mass (congression index) of HeLa cells with metaphase type chromosome alignment in the absence and presence of different c...

Ngày tải lên: 30/03/2014, 10:20

... steps: first, generation of multiple core mutants of the target protein; secondly, display of mutants on the phage surface; and finally, selection for stably folded mutants by challenging the system ... that proteins can be strongly stabilized by many different sets of surface mutations. Furthermore, the selection is found to be dependent on selection conditions. In...

Ngày tải lên: 19/02/2014, 12:20

... evalu- ated by measuring the effect of the phage on cAMP production. None of the ECL2-specific phage clones displayed agonistic properties. The antagonistic prop- erties of the selected HCDR3 phage ... distribution of the subpopula- tion of phage isolated after each selection round was monitored and compared with the length distribution of the initial library. Analy...

Ngày tải lên: 28/03/2014, 22:20

... two-step selection procedure from a random library of constrained peptides displayed on bacteriophages, based on exclusive affinity for theIgactivesite. Materials and methods Production and purification ... 7.5, containing 50 m M Tris/HCl and 150 m M NaCl. The library was stored at )20 °C. Titration of the library was performed by infection of mid-log phage E. coli bacter...

Ngày tải lên: 31/03/2014, 01:20

... strong conservation of the original LAC3 properties upon recombination, although the protein sequence of this hybrid is the least related to that of LAC3 (Fig. 5). On the other hand, LAC131 and ... rate of 4 mLÆmin )1 with a step gradient of NaCl: 0.1, 0.15, 0.2, 0.25, 0.3 and 1 m. Fractions containing laccase activity were pooled and concentrated to a volume of 600 lL by...

Ngày tải lên: 18/02/2014, 06:20

... and diploid cells [8,9]. During filamentation, Cdc42 regulates cell morphogenesis and invasion of the substratum [10]. Among the Cdc42p effectors that regulate cell polari- zation are Ste20p and ... growth. (A) Morphology of normal and hyperelongated cells. Exponentially growing cells carrying pGAL1-CLN1-3HA on a plasmid were induced by the addition of galactose for 4 h....

Ngày tải lên: 18/02/2014, 13:20

... tests and advised on prepa- ration of the final text, and to D. B. Robinson, Jr., L. Rosier, and B. J. Kinsberg for their contributions to selection of passages and preparation of questions ... values of a set of stimuli along a judged continuum is known to affect the distribution of responses for all stimuli in the set. The additional effect of context, on t...

Ngày tải lên: 19/02/2014, 19:20

... indicated by ‘+’) before methylation reac- tions using calf thymus histones as a source of the PRMT1 substrates histone 4 and histone 2A [18]. Inhi- bition by both compound 4 and AMI-1 was relieved by ... reduced the production of IFN- c from Th1 cells by more than 60% and the levels of IL-4 from Th2 cells by more than 75%, while incubation with AMI-1 reduced Th -cell c...

Ngày tải lên: 06/03/2014, 11:20