Báo cáo khoa học: Contribution of exofacial thiol groups in the reducing activity of Lactococcus lactis docx

... The role of the active mechanisms in maintaining a low reducing Eh7was thus investigated. Only the effects of compoundsthat could modify the cell activity were investigated. The activity of ... andincreased to 12 lm at the end of growth. The latter thiol concentration was correlated to the reducing activity. Indeed, a decrease in Eh7was linked to anincrease in exofacial thiol groups ... increase in accessible thiol groups on the cellsurface. The results obtained in the present study clearlyidentify the implication of exofacial thiol groups in the decrease in Ehand suggest that these...

Báo cáo khoa học: Monomeric molten globule intermediate involved in the equilibrium unfolding of tetrameric duck d2-crystallin pdf

... stabilityfor crystallins is essential in maintaining lens transparency[4]. Determining the mechanism of folding and assembly of these proteins is important for understanding how they canform stable ... concentrations. The d2-crystallin in lens was recruited during evolutionfrom argininosuccinate lyase, an enzyme involved in arginine biosynthesis derived from the urea cycle. Thesetwo proteins shared ... further increased. The unfolding curveusing the ANS ﬂuorescence probe suggested a multistateprocess, consistent with the results obtained by proteinintrinsic ﬂuorescence and CD. The binding...

Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx

... chain is brefeldin A-insensitive and isinhibited by the proteasome inhibitor clasto-lactacystinb-lactone, resulting in the accumulation of ricinA chains. These stabilized ricin A chains are partlydeglycosylated ... pivotal role of the UPR is to maintain ER home-ostasis. Therefore, the presence of mutated proteinsthat are unable to fold into their native conformation in the ER induces the UPR in an effort ... peptide in the 24-kDa a-zein and the 19-kDa a-zein endosperm storage proteins, respect-ively. These mutant proteins are translocated into the lumen of the ER, but remain anchored to the mem-branes...

Báo cáo khoa học: A cryptochrome-based photosensory system in the siliceous sponge Suberites domuncula (Demospongiae) docx

... expression in the vicinity of the spicules, particularly in the subsurfaceregions of the animals. In this context, signals weredetected intra- and extracellularly. Finally, usingwhole-mount in situ ... sequencing [55]. In mammals, the proteincryptochrome is one regulator in the complex molecu-lar system of the circadian clock [56].Focusing on the phylum Porifera, the closest relative of the ... resulting in a very weakbackground staining because of unspeciﬁc binding(Fig. 10C). Concurrent staining with 4¢,6-diamidino-2-phenylindole highlights the localization of nuclei and,consequently, of...

Tài liệu Báo cáo khoa học: Hypothalamic malonyl-CoA and CPT1c in the treatment of obesity pptx

... promoting an increase in proopiomelanocortin and cocaine and amphetamineregulated transcript. Taken together, these ﬁndingsprovide a compelling argument for the role for malo-nyl-CoA in regulating ... and certain amino acids [16].A linkage between fatty acid synthesis in the CNSand feeding behavior was uncovered with the ﬁndingthat systemic or intracerebroventricular administration of fatty ... needs to beexplored further. Other areas of the brain have alsobeen shown to regulate feeding via AMPK [35,36]. Of interest is the extensive use of fructose as asweetener in the human diet [37]....

Tài liệu Báo cáo khoa học: Evidence for noncooperative metal binding to the a domain of human metallothionein ppt

... investigated the metal-binding reactivity of the isolated a domain of human metallothionein isoform 1a, with speciﬁcemphasis on resolving the debate concerning the cooperative nature of the metal-binding ... the meta-llation mechanisms of the individual domains, in the hope, initially, of simplifying the interpretation of the metallation details of the two-domain protein. The results presented here ... bridging interaction in the a domain or to form another independent tetrathiolatesite in the b domain. It is probable that the equilib-rium constant for the coordination of the ﬁrst metalion in...

Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx

... ele-ments, very little is known about their role in DIDO activity. The combination of domains (domain shufﬂing)is a driving force in Eukarya in the acquisition of KeywordsCGBP; DATF1; DIDO1; DIO; ... from the other bran-ches, which was indeed predicted. The other maingroup, obtained 97% of the time in 20 740 exploredtrees, contained the DIDO PHD sequences clusteredwith the PHF and the ... analyses The localization of the DIDO1 gene in the context of chromatin stability was addressed experimentally.Although the experiments conducted were preliminary,they indicated the involvement of...

Báo cáo khoa học: Recent insights into cerebral cavernous malformations: the molecular genetics of CCM pot

... com-plete loss of any of the three CCM proteins within endothelial cells lining the cavernous capillary cavities. These data were an important step towards the elucidation of the mechanisms of this ... towards the understanding of the mechanisms of this disorder. Thisminireview summarizes the advances in CCM molecu-lar genetics and the remaining gaps in this ﬁeld. In addition to the identiﬁcation ... germlinemutations in CCM lesionsBased on the autosomal dominant pattern of inheri-tance of CCM and the presence of multiple lesions in familial CCM, contrasting with the detection of a sin-gle...

Báo cáo khoa học: Alpha 1,3-fucosyltransferase-VII regulates the signaling molecules of the insulin receptor pathway potx

... whether the SLexlevel of InR on insulin-responsive cells in diabeticpatients was changed. This may reveal the role of the sugar chains on InR in the pathogenesis of diabetes. In summary, the ... higher than in the correspondingcontrol cells cultured in the absence of insulin. The response to insulin stimulation was proportional to the expression of a1,3-FucT-VII. In insulin-stimulatedFucTVII-M ... 1 mg % of leupeptin andpepstatin). After protein determination, cell lysate contain-ing 500 lg protein was incubated with 5 lg of one of the following antibodies (antibody to InR-a or InR-b,...

Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterﬂy, Papilio xuthus docx

... staining of the gel indicates that the ﬂuorescing protein is one of the major components of soluble proteins in the crude extract. The surface of the ﬂuorescing protein carries negative charge in ... cellular retinal-binding protein; CRBP,cellular retinol-binding protein; IRBP, interphotoreceptorretinoid-binding protein; RBP, retinol-binding protein.Note: The nucleotide sequence reported in this ... ligand-binding protein in the ﬂy chemosensoryhair [26]). The calculated pI value of the Papilio RBP is 4.92; the protein is highly acidic. This explains the high mobility of the protein in the native...

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