Báo cáo khoa học: Crystal structure of human cystatin C stabilized against amyloid formation pdf

... of papain-like cysteine proteases. Abbreviations AS, appending structure; HCC, human cystatin C; HCCAA, hereditary cystatin C amyloid angiopathy; HCC-stab, monomer -stabilized human cystatin C; ... models of type 2 cystatins, such as chicken cystatin (PDB code: 1CEW), cystatin D (PDB code: 1RN7), or cystatin F (PDB code: 2CH9). In addition, it is possible to compar...

Ngày tải lên: 22/03/2014, 21:20

... La Jolla, CA, USA) with forward primer 5¢-GAAACGATGC ATTTGTCCTATGCCGACCGTGCGTC-3¢ and reverse primer 5¢-GACGCACGGTCGGCATAGGACAAATGCA TCGTTTC-3¢. The sequence of the second PCR product was also confirmed. ... significance of the lid-loop in GGT catalysis. During the preparation of this article, the crystal structures of Bacillus anthracis CapD, a GGT-related enzyme, in the absence and...

Ngày tải lên: 22/03/2014, 21:20

... structure of proinsulin C- peptide in aqueous solution and in the presence of lipid vesicles. Cell Mol Life Sci 57, 337–342. 27 Xie L & Tsou C- L (1993) Comparison of secondary structures of ... folded con- formations, it can be used to predict the secondary- structure content of the time or ensemble average. The differences DdH a of the measured chemical shifts from ran...

Ngày tải lên: 23/03/2014, 15:20

... pocket are completely different, possibly accounting for the clear differences in reaction kinetics between basidiomycete laccases and asco-laccases. Dimerization In the TaLcc1 crystal structure, ... basidio- mycete laccases, such as T. versicolor, L. tigrinus and C. cinereus laccases, have Asp residues here. In the crystal structure of the basiodiomycete T. hirsuta lac- case (PDB c...

Ngày tải lên: 14/02/2014, 18:20

... peptide complex (observed structure factors F pep o ) with an apo structure of importin-a (70–529) (observed structure factors F apo o ). This structure is described in ref. [42]. The structure factors ... integration of macromolecular diffraction data. Acta Crystallogr D: Biol Crystallogr 62, 48–57. 36 Collaborative Computational Project Number 4 (1994) The CCP4 suite: programs...

Ngày tải lên: 14/02/2014, 19:20

... metal cofactor, respectively. SODs are grouped into four classes according to their metal cofactors: copper and zinc-containing SOD (Cu,Zn-SOD), iron-containing SOD (Fe-SOD), manganese-containing ... first time, we describe the crystal structure of ApeSOD. In particular, we focus on the coordination of the metal cofactor in the active site as well as the changes it experiences in respo...

Ngày tải lên: 14/02/2014, 22:20

... the structure of PhyK and four in PhyK H25A), these structural differences are not caused by crystal lattice contacts. Another conformational change in AppA involves Glu219. The side chain of ... LaCount MW & Lebioda L (2003) Crystal structures of human prostatic acid phosphatase in com- plex with a phosphate ion and a-benzylaminobenzyl- phosphonic acid update the mechanisti...

Ngày tải lên: 16/02/2014, 09:20

... regulators of the proteolytic activity of DESC1. The reactive site loop sequences of PAI-1 and PCI fit to the active site geometry of human DESC1, without matching the optimal docking sequence of DESC1. ... main chain of the catalytic domain (B-chain) of DESC1 is well defined. Only a few side chains on the surface of the mole- cule are partially undefined in the electron densit...

Ngày tải lên: 19/02/2014, 00:20

... Deacetylase activity of the BcZBP protein on N-acetylchi- tooligosaccharides. Substrate Deacetylation (%) GlcNAc 100 (GlcNAc) 2 89.82 (GlcNAc) 3 10.51 (GlcNAc) 4 5.51 (GlcNAc) 5 12.19 (GlcNAc) 6 3.93 Fig. ... Model of the BcZBP–GlcNAc com- plex. Stereoview of the energy minimized putative BcZBP–GlcNAc complex. A slice through the active site cavity shows the quality of fit of the N-a...

Ngày tải lên: 19/02/2014, 00:20

... pooled and concentrated in centrifugal concentrators (Centricon and Minicon from Millipore) for crystallization trials. Crystallization and data collection Recombinant Vibrio proteinase was crystallized ... refinement of protein molecules. Acta Crystallogr Sect D 49, 129– 147. 78 Kleywegt GJ (1996) Use of non-crystallographic sym- metry in protein structure refinement. Acta Crystallogr Se...

Ngày tải lên: 19/02/2014, 16:20