Báo cáo khoa học: Characterization of an N6 adenine methyltransferase from Helicobacter pylori strain 26695 which methylates adjacent adenines on the same strand pptx

... Journal compilation ª 2010 FEBS 1683 Characterization of an N6 adenine methyltransferase from Helicobacter pylori strain 26695 which methylates adjacent adenines on the same strand Ritesh Kumar1, ... – one in the upper strand and one in the lower strand [24].Yetanother variation is seen in the case of MmeI, where ithas been reported that MmeI modiﬁes the adenine in the top strand of the ... concentration of [3H]Ado-Met in the range of 0.3–2.4 lm while keeping the DNAconcentration ﬁxed at 50 nm and keeping other reactionconditions identical. The nonlinear regression analysis of initial...

Tài liệu Báo cáo khoa học: Characterization of a chemosensory protein (ASP3c) from honeybee (Apis mellifera L.) as a brood pheromone carrier pdf

... Pheromones of Social Bees. Chapman & Hall,London.41. Brockmann, A., Bru¨ckner, D. & Crewe, R.M. (1998) The EAGresponse spectra of workers and drones to queen honeybee man-dibular gland ... probe concentration. The synthetic blend correspond-ing to the major components of the queen bee mandibulargland extract was purchased from Phero Tech Inc.(Canada). It is composed of 9-keto-2(E)-decenoic ... andb-ionone, which are known components of ﬂoral scents[59] and 2-heptanone, geraniol, citral, 2-nonanol andisoamyl acetate, known to be honeybee nonsexual phero-mones [40]. Cuticular hydrocarbons...

Báo cáo khoa học: Characterization of membrane-bound prolyl endopeptidase from brain ppt

... membranes presented two bands: the light band and the one that matched the soluble POPcontrol. On the other hand, in the light membranefraction, the latter band was the only one appearing.It was ... blot, 70 lg of TM, 96 lg of LM, 89 lg of MM, and 93 lgofHM; for the calnexin blot, 70 lg of TM, 96 lg of LM, 89 lg of MM, and 93 lg of HM; and for the ER-60 blot, 8 lgofMMand 5 lg of HM.Table ... observed forany of the substrates tested.Membrane PE probably associates with the membrane by nonprotein hydrophobic anchoring The nature of the association of membrane PE withmembranes is not...

Báo cáo khoa học: Characterization of myo-inositol hexakisphosphate deposits from larval Echinococcus granulosus pdf

... constituent of the deposits and show that its formation is predicted from thermodynamic constants under the relevant biologicalconditions.ResultsMild alkaline treatment of the E. granulosus cystwall ... InsP6and D- and ⁄ or L-Ins(1,2,4,5,6)P5, were identi-ﬁed on the basis of elution times in compar-ison with standards. The column used was an IonPac AS11, 2-mm bore, anion-exchange column. The ... precipitation, the single prediction obtained (throughout the range of pHand vesicle volumes) was the precipitation of 100% of the InsP6present as Ca5H2LÆ16H2O. This means that the observed...

Báo cáo khoa học: Interaction of an 40 kDa protein from regenerating rat liver with the )148 to )124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver pdf

... 4 C,D, lanes 2 and 3 in both panels). On the other hand, me thyl green did not affect the f ormation of the complexes (lanes 4 and 5 ). The insensitivity of nRLjunRPand rRLjunRP to the major ... Delhi, India The c-jun belongs to the family of proto-oncogenes andencodes for the protein Jun, a component of transcriptionfactor AP-1 involved in regulation of the expression of genesindispensable ... development andfunction of an organism. Major control of gene expressionis exerted by regulating m RNA p roduction and involvescomplex i nteractions between an array of t ranscriptionallyactive...

Báo cáo khoa học: Characterization of cinnamyl alcohol dehydrogenase of Helicobacter pylori An aldehyde dismutating enzyme docx

... pre-sent in each lane. The blot was developed by enhanced chemilumi-nescence: lane 1, strain 26695; lane 2, strain 1061; lane 3, strain G27 and lane 4, strain N6. Helicobacter pylori cinnamyl ... vary-ing concentrations of propanol at a ﬁxedconcentration of NADP+coenzyme showssubstrate inhibition occurring at concentra-tions above 50 mM propanol (inset). The type of inhibition by propanol ... Dismutation of benzaldehyde by HpCAD. The data shows the formation of benzoic acid as a function of time, at differentstarting concentrations of benzaldehyde (15, 10 and 5 mM). Afteraddition of the...

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... that there aretwo mechanisms inside the cell: one relative to the pro-duction of ML (and TG) with a rate constant Rpandone relative to the consumption of ML (and TG) witha rate constant ... [7].Modulation of ML intensity can then be ascribed to an equilibrium between the build up and consumption of these molecules due to the cellular state. In controlcells, these changes can only be ... that the variance of the two samplesis equal. By estimating the variance from the standard devia-tions and by using the F distribution [27], we could not ﬁndany statistical difference in the...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... characterizedby the degeneration of larval tissues, such as the velumand the foot, and the remodelling of larval tissues toproduce adult tissues (i.e. the development of the gillsand the production of an ... is the mean + SE of three pools of four animals (tissues) or the mean of one pool of embryos or larva from one spawn. Expression levels arerelated to 100 copies of GAPDH. (B) Localization of ... adult shell), which is accom-panied by signiﬁcant growth of the soft body parts[21]. The mantle edge governs shell formation andbody growth by the secretion of shell organic matrixand by cell...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... describe the identiﬁcation of a novel tran-scription starting exon, designated as exon 1a, inhuman DNASE1 and characterization of the promoterregions of the gene; Sp1 transcription factor plays an important ... nucleotide positions relative to the transcription starting site of exon 1. Constructs are shown in the left-hand panel; construct namesare given at the left of the bar and the locations of the inserted ... twotranscription starting exons in QGP-1 cells. Deletion of the upstream end of the 5¢-ﬂanking region of exon 1a from position )13952 to )11965 did not resultin any signiﬁcant change. However, deletion of...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... in the interactionwith the H+-ATPase and makes PPI1588His6a suitabletool to study the mechanism of action of PPI1. The analysis of the pH dependence of PPI1-induced activation of the ... withPPI1588His6. The analysis of the effect of PPI1588His6 on the dependence of PM H+-ATPase activity on the concen-tration of MgATP (Fig. 3) showed that stimulationdecreased with the increase of PPI1588His6concentra-tion. ... expression of the N-terminal portion of the pro-tein (His6PPI188) was done in the same conditions of PPI1588His6, but with 3 h of induction. Protein was puriﬁedessentially as described by the...

Xem thêm