Báo cáo khoa học: The pharmacological chaperone isofagomine increases the activity of the Gaucher disease L444P mutant form of b-glucosidase docx

... Therapeutics. Journal compilation ª 2010 FEBS The pharmacological chaperone isofagomine increases the activity of the Gaucher disease L444P mutant form of b-glucosidase Richie Khanna1, Elfrida R. ... wild-type human GCase activity in vitro [25]. The ability of IFG to cross the blood–brain barrierand to increase the activity of L444P GCase encour-Effect of isofagomine on L444P acid b-glucosidase ... Amicus Therapeutics. Journal compilation ª 2010 FEBSages further evaluation of this molecule for the treat-ment of neuronopathic forms of Gaucher disease. The administration of IFG to L444P...

Tài liệu Báo cáo khoa học: Altered deoxyribonucleotide pools in T-lymphoblastoid cells expressing the multisubstrate nucleoside kinase of Drosophila melanogaster doc

... humancells may affect the metabolism of the target cells inmore ways than just the therapeutic purpose of the introduced gene. The normal function of nucleosidekinases is to provide the cells with ... shown).In order to test the enzymatic activity of the Dm-dNK–GFP fusion protein and the level of nucleo-side kinase activity in the cells, we determined the phos-phorylation of deoxythymidine (dThd) ... washing were performedaccording to the instructions of the manufacturer of the membrane (Amersham). The washed membrane was ana-lyzed by PhosphorImager.Incorporation of precursors of macromolecularcell...

Tài liệu Báo cáo khoa học: "In-Browser Summarisation: Generating Elaborative Summaries Biased Towards the Reading Context" doc

... to the discovered themes. Finally, the V-matrix relates the original sentences to each of the themes. The point of the SVD analysis is to discoverthese themes based on co-variance between the ... differ-ent assumption about the nature of the reading con-text. Instead of taking the anchor text as an indicator of the user’s information need, it assumes that the top n themes of the reading context ... of membershipscores to each theme. The ﬁrst of the semantic space mapping methods,SVD-link, ﬁnds the theme that the anchor text be-longs to best. This is done by consulting the V-matrix of...

Tài liệu Báo cáo khoa học: "A Multimodal Interface for Access to Content in the Home" pdf

... understanding of system capa-bilities. Of the remaining 66% of inputs (2166) which were within the functionality of the system, 68% were in grammar. On the within functionality portion of the data, the ... sets of scenarios and then freely interacting with each system. For counterbalancing purposes, half of the subjects used the tablet and then the re-mote control and the other half used the ... considering the ASR perform-ance, it is important to note that 55% of the 3280 speech inputs were out of grammar, and perhaps more importantly 34% were out of the functional-ity of the system...

Tài liệu Báo cáo khoa học: "An Information-Theory-Based Feature Type Analysis for the Modelling of Statistical Parsing" docx

... 1.6808 0.6612Y= the first right brother of the current node 0.4730 1.1525 0.7502Y= the first left brother of the current node 0.5832 2.1511 1.2186Y= the second right brother of the current node ... 0.5044 0.2525Y= the second left brother of the current node 0.0949 0.6171 0.2697Y= the first right brother of the parent 0.1068 0.3717 0.2133Y= the first left brother of the parent 0.2505 ... relation0.5832(Y= the first left brother)SD=1 1.1598(Y= the parent)0.4730(Y= the first right brother)0.2505(Y= the first left brother of the parent)0.0949(Y= the second left brother)SD=2 0.6483(Y= the...

Tài liệu Báo cáo khoa học: Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas From target to transcript ppt

... if the actions of the CHH neuropeptides on repression of ecdysteroid synthesisby the Y-organ (YO) are considered. The most widely accepted paradigm of moult controlin crustaceans concerns the ... levels in the XO, and peptide levels in the SGduring these signiﬁcant stages of the moult cycle, as these are of fundamental importance with regard to the currentlyaccepted model of moult control. ... but as these experiments clearlyshowed reduction in biosynthesis of both ecdysteroids in the presence of either neuropeptide, reiterating the ﬁndings seenfor total ecdysteroids, further studies...

Tài liệu Báo cáo khoa học: "ENGLISH WORDS AND DATA BASES: HOW TO BRIDGE THE GAP" pdf

... independently of each other (one on the basis of the possible Eng- lish questions about the subject-domain, the other on the basis of the structure of the data base about it) there must be ... items of English. The prim- itives of the lowest semantic level are the prim- itives of the data base (names of files, attributes, data-items). The formal language used at this level is therefore ... meaning of the input question is represented by an expression of a formal logical language. The levels differ in that each of them assumes different semantic primitives. At the highest of these...

Báo cáo khoa học: CD91 interacts with mannan-binding lectin (MBL) through the MBL-associated serine protease-binding site doc

... effect (Fig. 4C). In the case of L-ﬁcolin, the interaction with CD91 was abolishedin the presence of EDTA, but the presence or absence of Ca2+ions in the buffer had no effect on the interac-tion ... LikeMBL and the ﬁcolins they are pattern-recognitionmolecules but do not share the property of binding the MASPs and therefore do not activate the complementcascade but function as opsonins. The lung ... neutralcarbohydrates [8].It is synthesized in the liver and,like the other ﬁcolins, has the potential of initiating the MBL ⁄ lectin complement cascade through the MASPs [12–15].SP-A and SP-D...

Báo cáo khoa học: Post-ischemic brain damage: targeting PARP-1 within the ischemic neurovascular units as a realistic avenue to stroke treatment pptx

... PARP-1-dependent derangement of the integrity of the neurovascular unit is caused by the enzyme’s ability to prompt an increase of blood–brain barrier (BBB) permeability, the release of pro-inﬂammatory ... occlusion[41]. The key role of PARP-1 hyperactivation in endo-thelial dysfunction in experimental models of diabetesunderscores the pathogenetic relevance of the enzymeto disorders of this key ... contribute to the synthesis and release of pro-inﬂammatory mediators [17]. It is now widely acceptedthat the latter are responsible for disruption of the capillary basal lamina, opening of the BBB...

Báo cáo khoa học: Insights into substrate and product trafﬁc in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel ppt

... analysis of initial rate data in the absence of inhibitors, we used the speciﬁc equation in Scheme 1. The effect of two ligands on the activity of the wild-type DmAChE was evaluated by the equation ... codes 1DX4 and 1QO9). One of these conformations results in the formation of a chan-nel approximately 5 A˚in diameter, connecting the gorge to the bulk solvent (Fig. 2A). The present study aimed ... to the PAS hinder the entrance of acetylcholine to the CAS in the wild-type enzyme, it appears that, in the two mutants(Trp83Ala ⁄ Glu), inhibitors that bind to the PAS didnot prevent the...

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