Báo cáo khoa học: Antimicrobial and conformational studies of the active and inactive analogues of the protegrin-1 peptide docx
Báo cáo khoa học: Antimicrobial and conformational studies of the active and inactive analogues of the protegrin-1 peptide docx
... Authors Journal compilation ª 2010 FEBS Antimicrobial and conformational studies of the active and inactive analogues of the protegrin-1 peptide Sylwia Rodziewicz-Motowidło 1 , Beata Mickiewicz 1 , ... second face of the peptide. Two hydrophilic regions were located at the two spatial tips of the molecule, at both termini with Arg1 and Arg17, and in...
Ngày tải lên: 22/03/2014, 21:20
Tài liệu Báo cáo khoa học: Antimicrobial effects of H4-(86–100), histogranin and related compounds – possible involvement of DNA gyrase ppt
... observed in the pres- ence of ATP. On the other hand, the bactericidal potencies of LL-37 (Fig. 2E) and protegrin (Fig. 2F) were not affected by ATP. Therefore, the antimicrobial activity of these ... first verified whether the antimicrobial activities of the peptides were affected by the presence of DNP or fluoroacetate. The two agents significantly blocked the...
Ngày tải lên: 18/02/2014, 14:20
Báo cáo khoa học: Adenine, a hairpin ribozyme cofactor – high-pressure and competition studies potx
... is unlikely on the basis of the results presented in the preceding section) or from re-equilibration of the reac- tion on the basis of the DV, the pressure increasing the rate of the ligation reaction. ... was then plotted as a function of time, and the kinetics were fit- ted to the exponential equation described above, allowing the estimation of the ini...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Escherichia coli cyclopropane fatty acid synthase Mechanistic and site-directed mutagenetic studies potx
... com- parable to the rate of inactivation of the His 6 -tagged wild- type enzyme, indicating that the Cys responsible for the loss of activity is not one of the conserved residues. Therefore, none of the ... devoid of organic or metal cofactors and is unable to catalyze the wash-out of the m ethyl protons of S-AdoMet to the solvent, data that do not support...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
... antimicrobial peptides, the granular glands of amphibian skin constitute one of the richest [14–16]. Studies on the mode of action of amphibian antimicrobial peptides have mainly addressed their interaction ... (p 0 ), the peptide (0.1–2 lm) was injected into the subphase, and the increment in surface pressure of the LPS film upon intercalation of the pep...
Ngày tải lên: 16/03/2014, 00:20
Báo cáo khoa học: Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate kinase potx
... T, and I N and I U are the intercepts and m N and m U are the slopes of the pre- and post-transitional base lines of the raw data, respectively. Quantitative analysis of the experimental data Thermal ... ques- tion of whether binding of a single substrate to one of the two domains can also stabilize the structure of the other domain [32–34]. Often the...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Lipid-induced conformational transition of the amyloid core fragment Ab(28–35) and its A30G and A30I mutants docx
... FEBS sity and a spectral blue shift of the peak maxima of 14, 8 and 19 nm for the WT ⁄ W27, A30G ⁄ W27 and A30I ⁄ W27 peptides, respectively (Fig. 4A–C). On the other hand, the spectra of the C-labeled ... that the binding of the amyloid protein of AD to lipid membranes can change their fluidity [34]. Therefore, we examined the effect of bind- ing of the W...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Antimicrobial peptides from hylid and ranin frogs originated from a 150-million-year-old ancestral precursor with a conserved signal peptide but a hypermutable antimicrobial domain pot
... 22-residue signal peptide and the first three residues of the acidic propiece and the second exon encodes the remainder of the acidic propiece plus the processing signal Lys-Arg and the antimicrobial peptide ... granules of these glands [70,71]. The glands may release their content onto the skin surface by a holocrine mechanism involving the rupture of...
Ngày tải lên: 23/03/2014, 17:21
Báo cáo khoa học: Leishmania donovani phosphofructokinase Gene characterization, biochemical properties and structure-modelling studies doc
... humans. The results of this work are presented in this paper, together with a report of the cloning and analysis of the PFK gene of the fish parasite Trypanoplasma borreli (a representative of the ... identical residues between either the set of kinetoplastid and E. histolytica ATP-PFKs and the set of typical ATP-PFKs, or the kinetoplastid and E. histolyti...
Ngày tải lên: 23/03/2014, 21:21
Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... against the existence of the complex and channeling of metabolites [10,11]. Thus, the existence of the com- plex has not been universally accepted. In addition to the cytoplasmic compartment, GAPDH and ... right. The distances between the N- terminal amino acids of subunits O and P, subunits O and Q and subunits O and R are shown (B–D). FLIM analysis of th...
Ngày tải lên: 16/02/2014, 09:20