Báo cáo khoa học: Antimicrobial and conformational studies of the active and inactive analogues of the protegrin-1 peptide docx
Báo cáo khoa học: Antimicrobial and conformational studies of the active and inactive analogues of the protegrin-1 peptide docx
... Authors Journal compilation ª 2010 FEBS Antimicrobial and conformational studies of the active and inactive analogues of the protegrin-1 peptide Sylwia Rodziewicz-Motowidło1, Beata Mickiewicz1, ... second face of the peptide. Two hydrophilicregions were located at the two spatial tips of the molecule, at both termini with Arg1 and Arg17, and in the turn in the presence of Arg8 and Arg10 ... families of these peptidesapplied mainly to the varied structures of the N- and C-termini. All the features of the structures calculatedfor all three peptides were in very good agreement withthe...
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Tài liệu Báo cáo khoa học: Antimicrobial effects of H4-(86–100), histogranin and related compounds – possible involvement of DNA gyrase ppt
... observed in the pres-ence of ATP. On the other hand, the bactericidalpotencies of LL-37 (Fig. 2E) and protegrin (Fig. 2F)were not affected by ATP. Therefore, the antimicrobial activity of these ... first verified whether the antimicrobial activities of the peptides were affected by the presence of DNP or fluoroacetate. The two agents significantlyblocked the antimicrobial activity of H4-(86–100)(Fig. ... C and histone H2A are released, and there-after histone H2A would be processed into buforin-I and fixed to the mucous surface of epithelial cells toprevent the entry of bacteria into the epithelial...
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Báo cáo khoa học: Adenine, a hairpin ribozyme cofactor – high-pressure and competition studies potx
... isunlikely on the basis of the results presented in the preceding section) or from re-equilibration of the reac-tion on the basis of the DV, the pressure increasing the rate of the ligation reaction. ... wasthen plotted as a function of time, and the kinetics were fit-ted to the exponential equation described above, allowing the estimation of the initial rates of the reaction. In the case of ... extract the values of the rate constant and of the apparent equilib-rium. However, it is well established that Mg2+induces the docking of loops A and B for the struc-tural organization of the...
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Báo cáo khoa học: Escherichia coli cyclopropane fatty acid synthase Mechanistic and site-directed mutagenetic studies potx
... com-parable to the rate of inactivation of the His6-tagged wild-type enzyme, indicating that the Cys responsible for the loss of activity is not one of the conserved residues. Therefore,none of the ... devoid of organicor metal cofactors and is unable to catalyze the wash-out of the m ethyl protons of S-AdoMet to the solvent, data that donot support the ylide mechanism. Inactivation of the enzymeby ... inactivation. Futhermore the plot a llows the estimation of s and p, as the o rdinate in tercept is (p+s)/p ¼ 2.1, the abscissa intercept is p+s ¼ 5 and the slope is)1/p ¼ –0.44. Thus p ¼ 2.3 and s ¼...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
... antimicrobial peptides, the granular glands of amphibian skin constitute one of the richest [14–16]. Studies on the mode of action of amphibian antimicrobial peptides have mainly addressedtheir interaction ... (p0), the peptide (0.1–2 lm) was injectedinto the subphase, and the increment in surface pressure of the LPS film upon intercalation of the peptide dissolved in the subphase was monitored for the ... wor-thy of remark that both fragments of esculentin pep-tide were found to affect the integrity of the microbialplasma membrane and the synthesis of the microbialcell wall. To the best of our...
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Báo cáo khoa học: Correlation between conformational stability of the ternary enzyme–substrate complex and domain closure of 3-phosphoglycerate kinase potx
... T, and IN and IUare the intercepts and mN and mUare the slopes of the pre- and post-transitional base lines of the raw data, respectively.Quantitative analysis of the experimental dataThermal ... ques-tion of whether binding of a single substrate to one of the two domains can also stabilize the structure of the other domain [32–34]. Often the results of studies with the solubilized PGK ... to these permanent bonds, there are further,changeable H bonds. The number and exact location of these bonds vary upon ligation with the substrates and with the conformational states of the...
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Báo cáo khoa học: Lipid-induced conformational transition of the amyloid core fragment Ab(28–35) and its A30G and A30I mutants docx
... FEBSsity and a spectral blue shift of the peak maxima of 14, 8 and 19 nm for the WT ⁄ W27, A30G ⁄ W27 and A30I ⁄ W27 peptides, respectively (Fig. 4A–C). On the other hand, the spectra of the C-labeled ... that the binding of the amyloidprotein of AD to lipid membranes can change theirfluidity [34]. Therefore, we examined the effect of bind-ing of the WT, A30G and A30I peptides on the fluid-ity of ... showing the formation of fibrils and aggre-gates of WT, A30G and A30I peptides in NaCl ⁄ Pialone (A–C) and in the the presence of PG (D–F) and PC (G–I). After 2 days of incu-bation in the absence...
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Báo cáo khoa học: Antimicrobial peptides from hylid and ranin frogs originated from a 150-million-year-old ancestral precursor with a conserved signal peptide but a hypermutable antimicrobial domain pot
... 22-residuesignal peptide and the first three residues of the acidicpropiece and the second exon encodes the remainder of the acidic propiece plus the processing signal Lys-Arg and the antimicrobial peptide ... granules of theseglands [70,71]. The glands may release their content onto the skin surface by a holocrine mechanism involving the rupture of the plasma membrane and the extrusion of the granulesthrough ... in the sequence in asingle step. Antimicrobial peptides are synthesized in the multinucleated cells of the dermatous glands of the skin and large amounts are stored in the secretory granules of...
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Báo cáo khoa học: Leishmania donovani phosphofructokinase Gene characterization, biochemical properties and structure-modelling studies doc
... humans. The results of this work are presented in this paper,together with a report of the cloning and analysis of the PFK gene of the fish parasite Trypanoplasma borreli (arepresentative of the ... identical residues between either the set of kinetoplastid and E. histolytica ATP-PFKs and the set of typicalATP-PFKs, or the kinetoplastid and E. histolytica ATP-PFKs and the setofPPi-PFKs. Residues ... to a band of approximately 1.7 Mb (Fig. 2, lanes 3 and 4), the size of the proposed fusion product of chromosomes 8and2 9intheL. mexicana group [20]. The amino-acid sequences encoded by the ORFs...
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Tài liệu Báo cáo khoa học: Loose interaction between glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells doc
... against the existence of the complex and channeling of metabolites [10,11]. Thus, the existence of the com-plex has not been universally accepted. In addition to the cytoplasmic compartment, GAPDH and ... right. The distances between the N-terminal amino acids of subunits O and P,subunits O and Q and subunits O and R areshown (B–D). FLIM analysis of the interaction between cerulean–GAPDH and citrine–GAPDH ... (in the case of cellsamples without coexpression of GAPDH) or from the cytoplasmic area (in the case of cell samples with coexpression of GAPDH) of cells, and are expressed as mean ± standard...
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