Báo cáo khoa học: Vitamin D stimulates apoptosis in gastric cancer cells in synergy with trichostatin A ⁄sodium butyrate-induced and 5-aza-2¢-deoxycytidine-induced PTEN upregulation ppt

... NaBu and 5-Aza. Results Vitamin D induced apoptosis in gastric cancer cells through PTEN upregulation Earlier studies revealed that vitamin D was able to induce cell differentiation and cell cycle arrest, ... 4. Vitamin D enhanced the apoptosis induced by TSA ⁄ NaBu. HGC-27 gastric cancer cells were treated with VD3 and 200 nM TSA (A) , or VD3 and 2...

Ngày tải lên: 22/03/2014, 21:20

... novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein Linda P. ... released to be further degraded via the b-oxida- tion cycle [1]. Defects in MCAD accordingly impair the ability to degrade fatty acids, and MCAD deficiency is the most frequently dia...

Ngày tải lên: 20/02/2014, 02:21

... there are several homocysteine-lowering agents available. Cobalamin and vitamin B 6 are admin- istered to patients with hyperhomocysteinemia caused by a lack of these factors, and vitamin B 6 is also ... pur- chased from Gibco Laboratories (Grand Island, NY, USA). Caspase-3 and PARP1 antibodies were purchased from Cell Signaling (Danvers, MA, USA). Antibody against a- tubulin w...

Ngày tải lên: 07/03/2014, 09:20

... RNA–DNA hybrids in vitro [17] and has been defined as a dsRNA and RNA–DNA hybrid-binding domain (dsRHbd) [18]. The function of the dsRHbd in eukaryotic RNase H1 has also been discussed. The dsRNA ... con- taining 6 m urea, loaded on to a HiTrap Desalting Col- umn, and desalted with buffer C. Purified recombinant proteins were analyzed by SDS ⁄ PAGE on a 10–20% gradi- ent gel...

Ngày tải lên: 30/03/2014, 11:20

... 5C ,D) . In addition, Ac-DEVD-CHO, a caspase-3 inhibitor, inhibited trog- litazone-induced adipocyte apoptosis and caspase-3 cleavage (Fig. 5E–G). The Bcl-2-dependent and caspase-3-dependent apop- tosis ... troglitazone-induced adipocyte apoptosis was, indeed, inhibited in the presence of GW9662. In addition, pioglitazone, another TZD, and PGJ2, which directly binds to and...

Ngày tải lên: 16/02/2014, 09:20

... treated with EGF and ADR as described in (A) , harvested at the indicated times after the addition of ADR, and used for immunoblot analysis of pro-caspase 9 and caspase 9. (C) Cells were treated ... treated with EGF and ADR as described in (A) . Lysates were prepared at the indicated times after the ADR addition and analyzed for caspase 9 activity by using a fluorometric...

Ngày tải lên: 19/02/2014, 06:20

... the incident wavelength. Diffraction spots were recorded on a MAR345 image plate using the oscillation method [36]. Data indexing and scaling were carried out with DENZO and SCALEPACK software, ... codon GCA, and the Thr225 codon ACT was changed into the Ala codon GCT. The mutagenized GAPDH gene fragments were then excised from the plasmid by digestion with SalI and SacI and u...

Ngày tải lên: 20/02/2014, 02:21

... rlinden@biof.ufrj.br Abbreviations: AIF, apoptosis inducing factor; DAPk, death-associ- ated protein kinase; DRP, DAPk-related protein kinase; FADD, Fas-associated protein with death domain; IAP, inhibitor of apoptosis; LEI, ... deficient in caspase-8, the forced multimerization of Fas-associated protein with death domain (FADD) induced caspase- independent cell death. No DNA fragment...

Ngày tải lên: 07/03/2014, 15:20

... cell survival, the Ras ⁄ Raf ⁄ MEK ⁄ Erk pathway plays a crit- ical role. Indeed, Ras-activated Raf operates by phos- phorylating and activating MAPK-Erk kinase-1 and -2 (MEK 1 ⁄ 2) [9,10]. MEKs have ... quantification of cells with fragmented and ⁄ or condensed nuclei was performed using Hoechst 33342 and the percentage of apoptotic cells was deter- mined. Data are means ± SD...

Ngày tải lên: 07/03/2014, 21:20

... an increase in the upper band detected within 1 min after addition of CCK-8 and a maximum reached within 5 min Fig. 1. Identification of CrkII in rat pancreatic acini (A, B) and CCK- dependent induction ... Jensen 3 and Luis J. Garcia-Marin 1 1 Departamento de Fisiologia, Universidad de Extremadura, Caceres, Spain; 2 Departamento de Bioquimica, Biologia Molecular y Genetica, Univer...

Ngày tải lên: 07/03/2014, 21:20