Báo cáo khoa học: Targeting mechanism of the retinoblastoma tumor suppressor by a prototypical viral oncoprotein Structural modularity, intrinsic disorder and phosphorylation of human papillomavirus E7 doc

... tumor suppressor by a prototypical viral oncoprotein Structural modularity, intrinsic disorder and phosphorylation of human papillomavirus E7 Lucı ´ a B. Chemes, Ignacio E. Sa ´ nchez, Clara Smal and ... surfaces in RbAB. We discussed a mechanistic model of the interaction of the Rb protein with a viral target in solution, integrated with s...

Ngày tải lên: 22/03/2014, 21:20

... Irikura D, Aritake K, Nagata N, Maruyama T, Shimamoto S & Urade Y (2009) Biochemical, functional, and pharmacological characterization of AT-56, an orally active and selective inhibitor of lipocalin-type ... Asp43 and Lys77 and as an acid to generate PGD 2 and PGF 2a in the absence of NADPH or NADP + and in the presence of NADPH, respectively. Abbreviations AKR,...

Ngày tải lên: 14/03/2014, 23:20

... technical assistance. Drs Kazu- hito Naka (Kanazawa University) and Yasuo Ariumi (Okayama University) are also thanked for their valuable input in this study. This work was supported by Grants-in-Aid ... T-pIC and M-pIC treatments induced the phosphorylation at Ser386 and Ser396 of IRF-3, and formed the dimeriza- tion of IRF-3 (Fig. 5A, B, lanes 1 and 2), and that NS3-...

Ngày tải lên: 18/02/2014, 16:20

... Suzuki 5 and Yasushi Kawata 3,4 1 National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan 2 Department of Food Science and Nutrition, Faculty of Human Life and Science, ... bound to the metal, in the company of a water oxygen, from the apical positions. The manga- nese was only 0.06 A ˚ out of the equatorial plane (Table 3). The...

Ngày tải lên: 14/02/2014, 22:20

... chromophores accessible to the quencher. The values of f a and K Q calculated from the data obtained at pH 7.2 were 74% and 8.6 M )1 and for those measured at pH 6.0 26% and 15.3 M )1 . As both parameters ... HpPex5p and correlate the fluorescence data with structural data, a molecular model of the HpPex5p was built based on the known structure of the N-term...

Ngày tải lên: 21/02/2014, 00:20

... showed affinities in the same order of magnitude. On the other hand, ADP, the product of the ATPase reaction, bound to CopB-B with approximately half of the affinity of ATP. AMP had a comparable ... to calculate the relative contributions of the states to the amplitude spectra )a 1 and )a 2 of the global fit as detailed in Appendix S1. The result of this c...

Ngày tải lên: 07/03/2014, 00:20

... nrdF + gene was sequenced by a primer walking approach. For DNA analysis, dnastar software (DNAS- TAR Inc., Madison, WI, USA) and clone manager 5.0 (Scientific & Educational Software, Cary, NC, USA) ... Bruker software, as described above. Analysis of metals Manganese and iron have been determined by GF-AAS and ICP-MS. [As a result of problems with the protein matrix in...

Ngày tải lên: 15/02/2014, 01:20

... the Protein Data Bank (accession numbers 2dt9 and 2zho). Determination of quaternary structure The subunit organization of TtAKb was analyzed by analyt- ical ultracentrifugation and gel filtration ... (AK; EC 2.7.2.4) is an enzyme that catalyzes the first committed step, the phosphorylation of the c-carboxyl group of aspartate, of the biosynthetic pathway of the...

Ngày tải lên: 18/02/2014, 08:20

... followed by the addition of excess unlabeled 5¢ exon product as the chase. (B) A plot of the disappearance of substrate in a normal reaction (no chase, closed circles) and reap- pearance of the substrate ... 1) [14,17,21]. Dependence of substrate cleavage on pH It has been reported that the rate of the substrate- cleavage step in Tetrahymena [22–25], Anabaena [...

Ngày tải lên: 18/02/2014, 18:20

... extracellular matrix, it seems more likely that the SEA domain functions by orienting the active site cleft of DESC1 toward plasma and ⁄ or extracellular spaces and away from the cell surface and ⁄ or the ... Marathon-Ready cDNA (Clontech, Moun- tain View, CA, USA). Two fragments were isolated and confirmed by Southern analysis using the internal cDNA fragment as the...

Ngày tải lên: 19/02/2014, 00:20