Báo cáo khoa học: Comparison of functional properties of two fungal antifreeze proteins from Antarctomyces psychrotrophicus and Typhula ishikariensis ppt

... et al. Antifreeze protein from ascomycetous fungusFEBS Journal 277 (2010) 394–403 ª 2009 The Authors Journal compilation ª 2009 FEBS 403 Comparison of functional properties of two fungal antifreeze ... biochemical properties and thermal hysteresis activity, and compared them with those of the T. ishikariensis antifreeze protein. The antifreeze protein from A. psychrotrophicus was puriﬁed and identiﬁed ... different from the T. ishikariensis antifreeze protein, and that these two psychrophiles have evolved from different genes.AbbreviationsAFP, antifreeze protein; AnpAFP, AFP from Antarctomyces psychrotrophicus; ...

Tài liệu Báo cáo khoa học: Understanding the binding properties of an unusual metal-binding protein ) a study of bacterial frataxin pdf

... A˚ and 2.6 A˚) and Glu19 (2.7 A˚), and two water molecules (1.7 A˚ and 1.9 A˚) (Fig. 4E). InM5, the side chains of Asp76 (1.9 A˚) and Asp27(2.4 A˚) of a symmetry-related molecule and ... sphere of M3 is completedby three other water molecules at distances of 1.6, 2.0 and 1.8 A˚, respectively, and by the carboxylate oxy-gens of Asp31 (2.1 A˚) and of Asp29 (2.7 A˚). The two aspartate ... concomitant appearance of other signalsin other parts of the spectrum. This result could be aconsequence of the paramagnetic properties of Fe(II),or it might arise from the presence of an intermediateequilibrium...

Tài liệu Báo cáo khoa học: Mapping the functional domains of human transcobalamin using monoclonal antibodies pptx

... directmeasurement of holo-TC in serum. The antigenicdeterminants and the functional domains of TC havenot been identiﬁed.Cloning [5–7] and recent expression of several kind-red Cbl-binding proteins ... absence of S-S bonds and the low number of conserved residuesin this part of the sequence. Only later did the largeN-terminal unit become involved in the binding of Cbl [13,14]. Assembly of two ... Speciﬁcity of monoclonal anti-(human transcobalamin) sera and their effect on the functional properties of transcobalamin. nd, notdone.mAbEpitopeclusterPrecipitation of Apo-TC (%)Precipitation of Holo-TC...

Tài liệu Báo cáo khoa học: Mapping the functional domain of the prion protein docx

... deletions of two of the helicaldomains of the PrP protein, also possessed a high content of helical structure.Activity domains of PrPThis body of research has provided two sets of resultsconcerning ... activity of PrP.Production of deletion mutants of PrPOn the basis of the results with antibodies, a series of PrPmutants were made to assess whether deletions of certaindomains of the protein ... PrPD35–45, PrP) and deletions of the C-terminal domain (PrP23–171). These proteins are illustrated in Fig. 2. A number of these proteins have been studied [28,32]. The identity of the proteins wasveriﬁed...

Báo cáo khoa học: On the aggregation properties of FMRP – a link with the FXTAS syndrome? pot

... described the biochemical and biophysicalaggregation properties of constructs spanning the conserved region of FMRP and of two other human fragile X related (FXR) proteins, FXR1P and FXR2P. Here, we ... and to premature ovarian failure infemales [12,13]. Two other proteins, fragile X related(FXR) proteins 1 and 2 (FXR1P and FXR2P), cancompensate partially for lack of FMRP in someorgans of ... forunderstanding granule formation and could have a linkwith the pathogenesis of FXTAS.ResultsFXR proteins present aggregation and amyloidogenic hot-spotsTo understand the aggregation properties of...

Báo cáo khoa học: Human anionic trypsinogen Properties of autocatalytic activation and degradation and implications in pancreatic diseases potx

... enlargement from the 120 min lanes of panel B,demonstrating the resolution of the four human trypsin(ogen) speciesin the gel. Tg, trypsinogen; bands A and B correspond to the two chains of double-chain ... trypsinogen Properties of autocatalytic activation and degradation and implicationsin pancreatic diseasesZolta´n Kukor, Miklo´sTo´th* and Miklo´s Sahin-To´thDepartment of Molecular and Cell ... (product of PRSS1, OMIM 276000),anionic trypsinogen (product of PRSS2, MIM 601564), and mesotrypsinogen (product of PRSS3)(forareviewonhuman trypsinogen genes and proteins see [1] and referencestherein)....

Báo cáo khoa học: "ON THE SUCCINCTNESS PROPERTIES OF UNORDERED CONTEXT-FREE GRAMMARS" ppt

... this claim rests on inspection of one of the standard proofs, say Hopcroft and Ullman [5]. The result is proved us- ing pushdown automata, but all conversions from pdas to grammars require ... the combinatory part of the proof. Let E and F be finite sets, and let J~ be a binary relation (set of ordered pairs) between E and F. R is said to cover F if every element of F participates ... z2, then one of the u-portions of the interchanged string wxz2yx will have two occurrences of that character, and thus not be in M(n~), as required by the IL. Without loss of generality,...

Báo cáo khoa học: Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64 ppt

... the surface of virions and infected cells [23]. To gain further insight intothe structural properties of gp64 and to increase theaccessibility of peptide insertions to their ligands, weinserted ... expression system foreukaryotic proteins in insect cell culture [1,2], for surfacedisplay of various peptides and proteins [3,4], and morerecently, for the transduction of mammalian cells [5–8].Baculoviruses ... generated by ligation of two fragments made by PCR with primers 64-394-SacII-back and 64-ELD-SacI-for and 64-ELD-SacI-back and 64-1536-BamHI-for (Table 1, section C). The two fragmentswere treated...

$Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic proﬁles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx$

Tài liệu Báo cáo khoa học: Comparison of membrane fraction proteomic proﬁles of normal and cancerous human colorectal tissues with gel-assisted digestion and iTRAQ labeling mass spectrometry pptx

... CRC.ResultsQuantitative analysis of membrane proteins from paired tumoral and adjacent normal tissue of CRC patientsA total of eight tumor tissues and eight matched normaltissues were collected from eight CRC ... (SLC25) consists of proteins that are functionally and structurally related and thatconstruct the transporters of a large variety of mole-cules [30]. Following LC-MS ⁄ MS and western blottinganalyses, ... quantitative analysis of membrane pro-teins of the CRC samples and reveals 215, 299, 191 and 208 proteins from four 4-plex iTRAQ LC-MS ⁄ MSexperiments, respectively. Statistical analysis of theexpression...

Tài liệu Báo cáo khoa học: Comparison of a coq7 deletion mutant with other respiration-defective mutants in ﬁssion yeast doc

... that induction of apt1 and and induc-tion of gpx1 depend solely on the Pap1 and Atf1 tran-scription factors, respectively, and that induction of ctt1 is dependent on both Pap1 and Atf1 in Sc. ... mix-ture of both authentic ubiquinone-10 and extracts from LN902(Dcoq7) yielded two separable peaks (Fig. 3A).The identiﬁcation of the main quinone-like compoundisolated from LN902 and authentic ... pombe Coq7, LN902(Dcoq7) wasFig. 1. Comparison of amino acidsequences of Clk-1 ⁄ Coq7p homologs.Alignment of COQ7 and its orthologousamino acid sequences from Sc. pombe(AL031854), S. cerevisiae...

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