Báo cáo khoa học: Functions and cellular localization of cysteine desulfurase and selenocysteine lyase in Trypanosoma brucei pot

... selenocystein lyase FEBS Journal 277 (2010) 383–393 ª 2009 The Authors Journal compilation ª 2009 FEBS 393 Functions and cellular localization of cysteine desulfurase and selenocysteine lyase in Trypanosoma ... Esaki N (1997) Cysteine sulfinate desulfinase, a NIFS-like protein in E. coli with selenocysteine lyase and cysteine desulfurase activities, gene...

Ngày tải lên: 22/03/2014, 21:20

... re-replication of DNA in a single cell cycle. Inactivation of CDK1 leads to re-replication of DNA in eukaryotic cells including human cells [7]. Targets of the kinase in the regulation of DNA replication include ... part of the MCM proteins on licensed chromatin in the G 1 phase was detached from chromatin. Phosphorylation of MCM4 and other fac- tors may be involved i...

Ngày tải lên: 16/03/2014, 13:20

... expression of protein H [23], while BMJ71 is deficient in both protein H and M1 protein [24]. In MC25, the COOH-terminal part of the emm1 gene of AP1 has been deleted resulting in a strain lacking cell ... constructs, spectinomycin (100 lgÆmL )1 )wasused. Proteins, GAGs, radiolabelling and binding assay Recombinant protein H, M1 protein and the A-S and S-C3 fragments of M...

Ngày tải lên: 20/02/2014, 11:20

... 15-kDa band, containing both the N- and C-terminal chains that are identical in size (band A). Activation of double-chain trypsinogen to double-chain trypsin results in the appearance of band B, ... conversion of trypsinogen to trypsin, as a faint trypsin band became apparent at 60 min, and more significant trypsin generation was detectable by 120 min. In the absence of Ca 2+...

Ngày tải lên: 17/03/2014, 03:20

... as shown in Figure 1 and 2. Taking advantage of this, we propose here a new method for analyzing the PBF diagrams by using natural language information. In the PBF, many kinds of information ... Process of PBF Diagram Understanding 4.1 Representation of Layout Information Layout information is represented by hand in the following way. Step 1. give ID number to all word...

Ngày tải lên: 17/03/2014, 07:20

... formation of a 40-kDa labelled protein in lanes 5 and 8 (containing the ab constructions) and proteins of 29 kDa in lane 11, and 11 kDa in lane 4 (containing, respectively, the a or b subunits). A band ... (a) and 11-kDa (b) subunit at 48 h and remained processed thereafter; the intensity of the bands increased at 72 and 96 h, in agreement with the enzyme being invol...

Ngày tải lên: 31/03/2014, 01:20

... structure, 2. appearing in the first sentence of some text in the training corpus, 3. exhibiting the same pattern as several expres- sions of type (2), 4. appearing in the corpus at least 5 times and always ... (“definites- only”). Using various combinations of these methods, D. Bean and E. Riloff achieved an accuracy for def- inite non-anaphoric NPs of about (F- measure), wit...

Ngày tải lên: 31/03/2014, 03:20

... APXs and Fig. 1. Alignment of amino acid sequences of C-terminal half-regions of ascorbate peroxidase (APX)-B and stromal APX of spinach. Recombi- nation sites for creating chimeric APXs are indicated ... lgÆml )1 was also included in the buffer to eliminate the possibility that small amounts of con- taminating proteins could interact nonspecifically with hydrogen peroxide and...

Ngày tải lên: 16/03/2014, 14:20

... position of Lys78 is anchored with an interaction between the main chain oxygen of Ala216 and the e-amine of Lys78. In H34A mutant (Fig. 6B), the interaction between the imidazole ring and Asp214 ... purified using nickel-affinity and gel-filtration chromatography. At this stage, sam- ples contained predominately fusion protein and a small amount of zymogen protein as determine...

Ngày tải lên: 23/03/2014, 07:20

... molecular complex. Interdomain contacts One of the measures of the extent of interactions between the domains of IN (dimerization of identical domains, and oligomerization of different domains) is the ... complexes of the IN- binding domain (IBD) of LEDGF with the CCD of HIV-1 IN [35], and with the NTD–CCD of HIV-2 IN [59]. The IBD used in these experiments in...

Ngày tải lên: 29/03/2014, 23:20