... ubiquitously in mammalian tissues[15]. Decreased activity of PKH leads inter alia to a decreased availability of PLP for GAD, which cata-lyses the formation of c-aminobutyric acid, the mostpotent inhibitory ... and c-aminobutyric acid) [7]. Onekey reaction is the formation of c-aminobutyric acidby decarboxylation of glutamate, catalysed by the twoisoforms of glutamate decarboxylase, GAD65andGAD67, ... with alkaline phosphase (Merck,Darmstadt, Germany; 100 U) at 37°C, as analyzed byHPLC.This material is available as part of the online articlefrom http://www.blackwell-synergy.comPlease note:...