Báo cáo khoa học: Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid pdf

... compilation ª 2010 FEBS 155 Structural characterization of N-linked oligosaccharides of laminin from rat kidney: changes during diabetes and modulation by dietary fiber and butyric acid Adishesha Puneeth ... N- linked oligosaccharides of laminin during diabetes and the likelihood of dietary fiber and butyric acid in mod- ulat...

Ngày tải lên: 22/03/2014, 17:20

... 1 molar ratio and then analysed by gel filtration chromatography. The peaks were concentrated using 3 lL of Strataclean protein bind- ing beads (Stratagene, Amsterdam, the Netherlands) and then ... consisting of four copies of four subunits, a, b, c and d. The a and b subunits are regu- latory and are the sites of phosphorylation and meta- bolite binding and are also re...

Ngày tải lên: 19/02/2014, 17:20

... and this was confirmed and detailed by NMR spectroscopy (see below). Characterization of OS fractions and LPS-OH by NMR The 1 H NMR resonances of OS fractions and LPS-OH were assigned by 1 H– 1 H chemical ... (b-configur- ation) or between H-1 and H-2 (a-configuration). On the basis of the chemical shift data and the large J 2,3 , J 3,4 and Table 4. Structures of th...

Ngày tải lên: 08/03/2014, 02:21

... e cloned and expressed the two functional regions of human Nogo-A and performed structural characterization by CD Correspondence to J. Song, Department of Biochemistry, National University of Singapore; ... been demonstrated to enhance CNS neuronal regeneration. (C) The amino acid se quence of the N-terminal 24 residues of Nogo-40. Fig. 2. Expression and purification o...

Ngày tải lên: 16/03/2014, 16:20

... 2,5-dichloro-p- benzoquinone at a concentration of 500 l M and ferricya- nide at a concentration of 2.5 m M and illuminated with saturating white light. Gel filtration chromatography Gel filtration chromatography ... and separated in a linear 0–1 M sucrose gradient. A pigment ratio of separated chlorophyll-con taining bands is indicated on t he right. (B) SDS/PAGE analysis of the...

Ngày tải lên: 16/03/2014, 18:20

... experiments. Interresidue NOEs were seen from H1B to H4C andtoitsownH2B,fromH1A to H2A and across the glycosidic bond to H3B,andalsofrom H1C to H3C and H5C and across the ring to H4A.HMBC experiment ... followed from H1B to H2B and H3B, and from the methyl resonance of H6B to H5B with no evidence of connectivities to any proton signals at C4B. The presence of a C4 keto g...

Ngày tải lên: 17/03/2014, 10:20

... comparisons of the funnels of LGOX, LAAO, and PAO, and the residues composing two entrances of the funnel of LGOX. (A) Stereo view of funnels of LAAO, PAO, and LGOX. The surfaces of the enzyme ... primary structure of LGOX exhibits approximately 20% and 12% identity, respectively, with those of LAAO and PAO. The alignments of sequences and secondary structure e...

Ngày tải lên: 23/03/2014, 05:22

... domain of dynamin [7]. The above possibility of functional dissection of the dynamin protein into specific domains suggests that a detailed characterization of the intrinsic structural and dynamic ... small propensity for formation of short helices, transiently, and the chain as such is highly dynamic. Structure of the core of the oligomers Because of the large size of...

Ngày tải lên: 23/03/2014, 11:20

... protein and ligand concentrations, DG and DH were calculated assuming 5% lower or higher con- centrations of protein and ligand (highly conservative esti- mates for the errors on these concentrations). ... FEBS Results Structure of unliganded PAL and its complex with mannose A correct understanding of ligand binding not only requires structural data on the protein-carbohydrate...

Ngày tải lên: 23/03/2014, 11:20

... aniso- tropy of the free minifiber, A B is the anisotropy of the mini- fiber bound to the penton base, [Lig B ] is the concentration of the bound ligand (minifiber), and [Lig tot ] is the total con- centration ... acid ‘minifiber’ construct was used as a fiber protein mimetic. Previous structural studies of hAd2pb with a 22 amino acid peptide from the N-terminus of the...

Ngày tải lên: 19/02/2014, 06:20