... Matsubara, Hisato Kuniyoshi and Susumu IkegamiDepartment of Applied Biochemistry, Hiroshima University, JapanWe report the constitution and molecular characterization of a novel tran sglutaminase ... that of the catalytic site-containing region of the other TGs wasamplified. To obtain further sequences upstream of primerTG5 and downstream of primer TG3V, we used the r apidamplification of ... (lanes 1–3 and 7–9) and nuclear fractions (lanes 4–6 and 10–12) we re prepared from 8-h-old early b lastulae (lanes 1, 4, 7, and 10), 12-h-old mid-blastulae (lanes 2, 5, 8, and 11), and 24-h-old...