Báo cáo khoa học: Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor potx

... dimeric RNases.AbbreviationsBS -RNase, bovine seminal RNase; ds -RNase A, domain-swapped RNase A; FAM-AUAA-TAMRA, 6-carboxyﬂuorescein-dArU(dA)2-6-carboxytetramethylrhodamine; RATE, RNase A tandem ... monomeric RNase A. On the other hand, the decreased activity of the RATEsas compared with RNase A ([16] and Table 1) indicate a negative inﬂuence of the tandemization on the cata-lytic efﬁciency. The ... FEBS Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor Ulrich Arnold, Franziska Leich*, Piotr Neumann, Hauke Lilie and Renate Ulbrich-HofmannDepartment...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... atoms) and rjis the standard deviation of Bfactors. The normalized B factors have a zero mean and unitvariance. All atoms that satisfy Bz‡ 4 are treated as outliers and discarded. After ... residue at P4, with a contact area of 13.5 A ˚2 and the Lys residue at P3 with a contactarea of 10.2 A ˚2. Residues outside the central bindingarea have signiﬁcantly lower values. These results ... by the importin -a nuclear import pathway, provided that CLIC4 can undergo a conforma-tional rearrangement that exposes the NLS in an extended conformation.DatabaseStructural data are available...

Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx

... oligonucleotides: a sense primer 5¢-CAACAAATTTGCATATGACTATTAAAG-3¢ containing an NdeI site (underlined) next to the start codon of the T. brucei gGAPDH gene; an anti-sense primer 5¢-CAGCCAAGCGCCTAGGGAGCGAGAAC-3¢, ... Mikaelian & Sergeant [33] and using the Vent DNA polymerase. The T. brucei gGAPDHThr196 ACA codon was changed into the Ala codon GCA, and the Thr225 codon ACT was changed into the Alacodon ... the T. brucei and T. cruzi proteins. These Kdvalues actuallyrepresent the ligand afﬁnities for a nonactive conformation of the enzyme in the absence of substrate and cofactor.Molecular...

Báo cáo khoa học: Identiﬁcation of microsomal rat liver carboxylesterases and their activity with retinyl palmitate potx

... microsomal rat liver carboxylesterases and their activity with retinyl palmitateSonal P. Sanghani, Wilhelmina I. Davis, Natividad G. Dumaual, Alan Mahrenholz and William F. BosronDepartment of Biochemistry ... protein with coomassie blue and the duplicate halfwas stained for activity as described for nondenaturingPAGE. The pI for each isoenzyme was calculated from the standard curve and the average of the ... from preparativePAGE (Table 1) and it appears as a doublet as shown inFig. 4. The relative abundance of these bands varies frompreparation to preparation. In one preparation where the bands were...

Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... both the size and the shape of the pocket (Fig. 5A, B). Leu297in TaLcc1 (Ala297 in MaL) narrows the cavity ascompared with MaL, whereas Pro195 and Val428(Phe194 and Phe427 in MaL) make the cavity ... (asco-lac-cases) have been rather unexplored, and to date only the crystal structure of Melanocarpus albomyces laccase (MaL) has been published. We havenow solved the crystal structure of another ... weak homodimer, as observed for MaL, that may determine the properties of these asco-laccases at high protein concentrations.DatabaseStructural data are available in the Protein Data Bank database...

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... trigonal bipyramidal coordination with ﬁve ligands: four sidechain atoms and a water oxygen. One aspartate and two histidine sidechains ligated to the central metal on the equatorial plane. In the ... bound to the metal, in the company of a water oxygen, from the apical positions. The manga-nese was only 0.06 A ˚out of the equatorial plane(Table 3). The angles around the metal cofactor sug-gested ... hyper-thermophilic crenarchaeon, Aeropyrumpernix K1. DNA Res 6, 83–101.11 Nakamura T, Yamamoto T, Inoue T, Matsumura H,Kobayashi A, Hagihara Y, Uegaki K, Ataka M, Kai Y& Ishikawa K (2006) Crystal...

Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... and hydrolyze metal-free phytate with pH optima in the acidic range. They consist of two domains, a large a ⁄ bdomain and a small a domain with the catalytic site at the interface of the two domains [4,5]. ... substrate-freeAppA the C a atoms are 2.41 A ˚apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1.87 A ˚.Distinct conformational changes ... unit.Single-wavelength anomalous diffraction data at the Seedge of a Mse-derivatized crystal of the same space group and with similar cell dimensions were collected at BL 14-1at BESSY. Diffraction data...

Tài liệu Báo cáo khoa học: Crystal structure of the catalytic domain of DESC1, a new member of the type II transmembrane serine proteinase family pptx

... and the human airway trypsin (HAT) ⁄ HAT-like ⁄ DESCsubfamily. Members of the hepsin and matriptase subfamilies are knownstructurally and here we present the crystal structure of DESC1 as a ... regions of these proteinases medi-ate interaction of the two b-barrels, formation of the catalytic machinery and structures required for binding of the main chain of the substrate peptide and properpositioning ... extracellular matrix, it seems more likely that the SEA domain functions by orienting the active site cleft of DESC1 toward plasma and ⁄ or extracellular spaces and away from the cell surface and ⁄ or the...

Tài liệu Báo cáo khoa học: Crystal structure of the BcZBP, a zinc-binding protein from Bacillus cereus doc

... Ivanova N, Sorokin A, Anderson I, Galleron N,Candelon B, Kapatral V, Bhattacharyya A, Reznik G,Mikhailova N, Lapidus A et al. (2003) Genomesequence of Bacillus cereus and comparative analysis with ... hydrogen-bon-ded antiparallel b-strands (b6–b7), an a- helical hairpin(helices a5 , a6 ) and a C-terminal strand (b8). Helix a5 and strand b7 partially cover one side of the Ross-mann fold structure, thereby ... present the crystal structure of BcZBP at 1.8 A ˚resolution. The N-terminal part of the 234amino acid protein adopts a Rossmann fold whereas the C-terminal partconsists of two b-strands and two a- helices....

Tài liệu Báo cáo khoa học: Crystal structure of a subtilisin-like serine proteinase from a psychrotrophic Vibrio species reveals structural aspects of cold adaptation docx

... extreme thermal motion. As a response, they have evolved enzymes that are highlystable against heat and other denaturants. The increased stability of enzymes from thermo- and hyper-thermophiles ... positions and gave a root mean square deviation of 0.84–1.21 A ˚(Table 2, Fig. 2). The structural resemblance with regard to root mean square deviation, fraction of com-mon Ca-atoms and the amino acid ... helix A and residues of the succeeding surface loop and it iscoordinated by the side chain and carbonyl oxygen of Asp9, the side chains of Asp12, Gln13, Asp19, the car-bonyl oxygen of Asn21 and...

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