Báo cáo khoa học: Peptides that bind the HIV-1 integrase and modulate its enzymatic activity – kinetic studies and mode of action pptx

... increased the K m of the IN–DNA interaction, and were thus inhibitory; (b) peptides that slightly increased the K m of the IN–DNA interaction, but in addition modified the V max and K cat values of the ... 3¢-end processing and strand-transfer assays were performed to study the mode of action of these peptides. The effects of the various peptides...

Ngày tải lên: 22/03/2014, 16:21

... similar to that of aB-crystallin [28]. MTB HSP 16.3 of M. tuberculosis may be ideally suited for studies of the structure and function of the core Ôa-crystallinÕ domain of sHsps because the quaternary structure ... vivo studies of specific inter- actions between MTB HSP 16.3 and other latent stage proteins will lead to a b etter u nderstanding of the molecular c...

Ngày tải lên: 23/03/2014, 21:21

... zinc and MT protect the liver and the heart against the toxic effects of ethanol [4 5–4 7]. The above results suggest that a critical aspect of the protective function of MT is the scavenging of the ... demonstrates that elevated levels of aldehydes affect zinc metabolism and that zinc release and ensu- ing binding of zinc to other proteins is one asp...

Ngày tải lên: 19/02/2014, 06:20

... addition of ISP and Qcr10p). On the other hand, in the transition from the 500 kDa band to the 670 kDa band, a structural rearrangement of the bc 1 complex may occur due to the binding of ISP and ... leu 2–3 ,112, can 1–1 1, qcr9D2::HIS3 [53] DISP MATa, ade 2–1 , his 3–1 1,15, trp 1–1 , leu 2–3 ,112, ura 3–1 , can 1–1 00, rip1D::LEU2 Present study DBCS1 M...

Ngày tải lên: 18/02/2014, 08:20

... nuclear fraction (NF) (lane 2), but not in DNA affinity- and Mono Q-fractions that EJ activity are enriched (lanes 3 and 4). On the other hand, the ligase components were found in a Heparin column fraction, ... [23, and ref- erences therein]. DNA aggregation activity is coupled with EJ activity To further confirm the relationship between protein– DNA aggregation and EJ ac...

Ngày tải lên: 19/02/2014, 06:20

... further understand the structure–potency relation- ship of the FAS inhibitory peptides, we examined the relationships between the physicochemical and biochemical features of these peptides and their ... RKQEEDEDEEQQRE and C75 and their interaction energies with the TE domain showed a strong correlation (r = 0.99). Close-up views of the binding modes of SBC-deriv...

Ngày tải lên: 06/03/2014, 09:22

... of these changes occurred at frame- work 3 of the heavy chain (Asp74Asn) and 3 of the light chain. Two of the changes (Thr152Ile and Ser197Gly) occurred at frameworks 1 and 3, respectively, and ... process begins with the cloning and expression of cDNAs encoding the variable regions of the H and L chains of antibod- ies (V H and V L ), allowing the in...

Ngày tải lên: 23/03/2014, 13:20

... reasonable trial has demonstrated that its benefit has passed the point of diminishing returns. However, there is a continuing trend of surrogates demanding that moribund patients be kept on ... maintaining some of the vital functions of the body, but the same technology does not necessarily allow us to heal underlying disease processes [2]. An unintended side effect of...

Ngày tải lên: 25/10/2012, 10:45

... embryo- genesis, all the evidence suggests that sex determination might disobey the conventional rules of evolutionary conservation. The common picture emerging here is that the genes at the top of the cascade ... result, the common emerg- ing picture is that the genes at the top of the cascade are not conserved, whereas the downstream genes have homologues i...

Ngày tải lên: 14/02/2014, 19:20

... the condensation and fragmentation of their nuclei. The percentage of apoptotic cells was calculated as the number of apoptotic cells divided by the total number of cells counted. A minimum of ... lost. Taken together, our results demonstrate an impor- tant relationship among the regulation of nucleo- lin ⁄ C23, the activation of caspase-3 and the induction of...

Ngày tải lên: 16/02/2014, 09:20