Báo cáo khoa học: Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding pot

... RNA binding and protein folding in RNA UDE. Interestingly, treatment of RNA UDE with RNa- se A did not result in significant changes of the melting curve, indicating that the characteristics of the ... are in excel- lent agreement with the hypothesis of the two confor- mational states presented in Fig. 4, and are also in line with the findings...

Ngày tải lên: 22/03/2014, 16:21

... Vg1RBP-associated proteins, such as the embryonic poly(A) binding protein, the Y-box RNA- packaging protein 2B and the oocyte-specific version of the elongation factor 1a (42Sp50). Incorporation of 42Sp50 into ... Vg1 mRNA in the context of the late vegetal RNA transport pathway. In the nucleus, Vg1 is recognized by the RNA- binding pro- teins Vg1RBP and...

Ngày tải lên: 23/03/2014, 03:20

... change with tissue source and age, but within a single chain there is vari- ability. The chain cap of CS is a GalNAc or GlcA residue; a 4,6-disulfated GalNAc residue, rare in the repeat region of ... diverse tissue sources, from tissues of differing ages and also within a single chain. Enhanced availability of data to facilitate the characterization of these struct...

Ngày tải lên: 16/03/2014, 14:20

... several proteins. The growth inhibitory proteins, Rassf1 and Nore1 can form complexes with and inhibit Mst1 activity in an interaction involving their conserved C-termini [15,25]. Interestingly, ... advantages in mutant versus normal tissue [17,18]. The Salvador protein has domains that permit protein protein interaction, including a WW domain and a predicted coiled-coil...

Ngày tải lên: 19/02/2014, 06:20

... expressed in the central regions of the brain, including the hypothalamus where it is an important regulator of food intake. The MC4 receptor shares 60% of the amino acids with the MC1 receptor, but has ... centrifuged. The intracel- lular cAMP produced was measured in 50 lLofthe supernatant after addition of [ 3 H]cAMP and bovine adrenal binding protein a...

Ngày tải lên: 21/02/2014, 00:20

... 455 The position of the AU rich region at the 5¢ end is not critical for Hfq binding The idea that stimulation of poly(A) synthesis is cor- related with the association of Hfq to the 3¢ end of RNA ... suggested that Hfq binding is independent of the location of the AU rich region in the RNA. This conclusion has been verified by comparing the affi...

Ngày tải lên: 19/02/2014, 16:20

... evidence in support of binding of ATP to the ligand-free hGK, resulting in a change of protein conformation. The MD simulations indicate that the binding triggers molecular motion of the flexi- ble ... associated with ATP binding; (c) obtaining insights into the active site contact residues involved in ATP binding; and (d) relating this informa- tion to steady-s...

Ngày tải lên: 06/03/2014, 00:20

... determine the area of the peaks after elution from the column. These analyses showed that, within the accuracy of the area determination, there was a linear rela- tionship between peak areas and the ... cleft is tunnel-like, permitting tight interaction with the polymeric substrate. The aglycon- side of the substrate-binding groove is extended by the aromatic...

Ngày tải lên: 07/03/2014, 16:20

... observable fluorescence band and a change in electrophoretic mobility due to the drug binding. This is attributable to the property of DAPI that favours folding of the random coil ATT strand into a fast-migrating ... of the free monomeric random coil structure of (ATT) 6 in comparison with its hairpin conformer bound to DAPI [16]. Binding mechanisms of DAPI and...

Ngày tải lên: 16/03/2014, 23:20

... increased (Fig. 2B). Pex21p increases tRNA binding by SerRS To further investigate the in uence of Pex21p on tRNA binding by SerRS, we compared the extent of tRNA binding by isolated enzyme and ... associate with aaRSs. Yeast TyrRS interacts with Knr4, a protein involved in cell wall synthesis [29], and heat shock protein 90 interacts with human glutamyl-prolyl-tRNA...

Ngày tải lên: 23/03/2014, 09:20