Báo cáo khoa học: The chromosomal protein HMGN2 mediates lipopolysaccharide-induced expression of b-defensins in A549 cells potx

... alone. The kinase-deficient mutant, OXI1 K45R , in which the lysine residue of the ATP bind- ing domain is mutated to arginine, still interacted with PTI1-4. These data indicate that the kinase ... tenth of the input was loaded on to the gel and represents the amount of HIS-tagged proteins used for the assay. The in vitro binding assays were repeated twice using rec...

Ngày tải lên: 14/02/2014, 19:20

... of the precise residues and interac- tions involved in the NTD–CTD interface [46]. Most of these involve side chains from helix 8 of the CTD which pack against the N-terminal end of helix 4 of the ... of the NTD of a neighboring subunit in the hexamer. Additional contacts involve helix 11 of the CTD and the C-terminal end of helix 7 of the NTD of...

Ngày tải lên: 18/02/2014, 06:20

... of both the CAI peptide and a-helix 9 of CTD during formation of the peptide– protein and protein protein complexes. These NMR experiments also allowed the determina- tion of the CTD binding site. ... protein (MA), the capsid protein (CA), the nucleocapsid protein (NC) and p6 proteins, as well as two spacer pep- tides. Currently, most of the available drugs...

Ngày tải lên: 18/02/2014, 06:20

... alcohols [29,30]. The previously published characterization of the lipid ⁄ protein interface of the nAChR [23] including the total number of n ¼ 40 lipid- binding sites of the nAChR [19,42] was ... of the nAChR over all binding sites: m ¼ 40 (solid line), or over part of the binding sites: m ¼ 10 (dotted line), and m ¼ 4 (dashed line). The data points for enhance...

Ngày tải lên: 19/02/2014, 18:20

... multispan- ning membrane proteins, we have investigated the mechan- ism of how the sensor kinase protein KdpD inserts into the membrane. We found that KdpD inserts into the membrane independently of the ... monitoring the accumulation of proOmpA. (B) Proteinase K mapping of the epitope-tagged KdpD protein in the YidC depletion strain, JS7131. Cells were grown...

Ngày tải lên: 20/02/2014, 11:20

... within the infected host cell. This minireview focuses on the proteomics of the capsid protein, its in uence on the packaging of nonviral molecules into HIV-1 virions and the subsequent role of the ... generate the correct conformation of pro- line, which is a rate-limiting step in protein folding. This ability possibly in uences the role of these enzymes in...

Ngày tải lên: 07/03/2014, 00:20

... effects. The [2Fe)2S] cluster of the Rieske protein is inserted in the cytoplasm As cytosolic cofactor insertion is one of the hallmarks of Tat translocation, the cofactor loading status of the ISP in ... fractions of the C152S mutant, demonstrating: (a) the inability of the mutant to insert a cofactor, and (b) the specific origin of the signal in t...

Ngày tải lên: 07/03/2014, 11:20

... proapoptotic protein of the Bcl-2 family that is crucial for death receptor-mediated apoptosis in many cell systems [16,17]. The BH3 domain-only proteins are a subfamily of the Bcl-2 family involved in the ... produced (Fig. 1D, lane 2). In the presence of lactacystin, a sig- nificant increase in the amount of tBid–GFP present in the extract, both in control...

Ngày tải lên: 16/02/2014, 09:20

... attributed to the physical prox- imity of the enzymes in the mini-cellulosomes and to cellulose targeting of the complexes via the CBM of the mini-scaffoldin [21]. The most active mini-cellulo- some ... (protein 12) is also present in noticeable quantities in the other five fractions. All the proteins present in F5 were also present in F6. Among these, we det...

Ngày tải lên: 18/02/2014, 08:20

... therefore, the E. coli machinery is able to rec- ognize the lipoyl-domain of the Thermoplasma E2 enzyme, the target lysine of which is flanked by D and V residues, as it is in the E2 protein of ... protein of the E. coli OGDHC and of the Bacillus subtilis BCOADHC (Fig. 1). How- ever, in addition to the identity of the neighbouring residues, it is the exa...

Ngày tải lên: 16/03/2014, 05:20