Báo cáo khoa học: Identification of malic and soluble oxaloacetate decarboxylase enzymes in Enterococcus faecalis potx

... including the catalytic tyrosine and lysine resi- dues involved in the acid–base mechanism, the divalent cation-binding residues, and two Rossman domains (GXGXXG) implicated in cofactor binding ... citrate) into pyruvate in the presence of divalent metals and in the absence of nicotinamide cofactors [7]. Noteworthy, the activity of MEF proteins contribute to the intracellu...

Ngày tải lên: 22/03/2014, 16:20

... the absence of an a-glucose residue that is often found substituting the 3 position of the Hep II residue in mAb B5 nonreactive strains such as immunotype strains L2 [5] and L5 [8] and clinical strain ... obtained from the LPS-OH of strain 1000 and strain NM115. Chemical shifts for the Gal II residue of strain 1000 were different because in this strain, Gal II is a terminal...

Ngày tải lên: 08/03/2014, 02:20

... Competition of recombinant PLA 2 toxins with 125 I-AtxC for binding to CaM. CaM was incubated with the labelled AtxC in the presence of increasing concentrations of the indicated competitor PLA 2 toxins, ... 20 residues [13] indicates that this portion of the C-terminal domain of CaM is exposed in the complex, which favors involvement of the N-terminal methionine-rich pocket...

Ngày tải lên: 17/03/2014, 03:20

... 1640 lacking cysteine and methionine and supplemented with dialyzed 10% fetal bovine serum, 100 IU penicillin/streptomycin, and 50 lCi ( 35 S)methionine and ( 35 S)cysteine was added, and the ... UÆmL )1 )and phytohemagglutinin (5 lgÆmL )1 ) in RPMI 1640 containing 10% fetal bovine serum and 100 IU penicillin/streptomycin. CHO-TRVb [human transferrin receptor (TfR)1-deficient C...

Ngày tải lên: 30/03/2014, 20:20

... outputs a sequence of tags according to the usual equation where and The tagger has been trained in the usual way, using part of the training data as heldout data for smoothing of the two models ... (Ngai and Yarowsky, 2000) and (Ngai, 2001) provide a thorough description of many experiments in- volving rule-based systems and statistical learn- ers for NP bracketing. For t...

Ngày tải lên: 08/03/2014, 05:20

... be important for exposing an aspartate-activating binding site [5]. By contrast, succinate and fumarate strongly increased the activity of NADP-ME2 in both direc- tions of the reaction (Figs 4 and 6). However, ... least in triplicate and the error bars indicate SD. Significant inhibition (as indicated in Materials and methods): dark grey and single-hatched bars. Significant...

Ngày tải lên: 23/03/2014, 04:20

... DNA-binding motif in nucleolin [21– 27]. The RGG domain of FMRP, which is an RNA- binding protein involved in nerve cell differentiation, interacts with the G-quartet forming RNA [22–25]. In addition, ... the guanidini- um group of the Arg in the RGG domain in the C-terminal of EWS. Results and Discussion Several DNA-binding proteins that bind to G-quadru- plex DNA have been...

Ngày tải lên: 15/02/2014, 01:20

... PCR identification using primers b1X and b1B, and the inactivated lpxX mutant was verified by sequencing. Cloning of lpxL and construction of the knockout mutant O35ElpxL A DNA sequence containing ... two mutants in bacterial clearance might reflect differences in the integrity of their outer membrane, binding activity and sensitivity of the murine comple- ment-mediated kil...

Ngày tải lên: 18/02/2014, 14:20

... number of genes encoding structural components of the cytoskeleton and proteins involved in contractile functions (including actin, tubulin myosin and profilin) were differentially expressed in C. ... known to involve major adjustments in the expression of ribosomal genes and genes coding for proteins involved in RNA metabolism and protein synthesis. In fact, protein sy...

Ngày tải lên: 18/02/2014, 16:20

... determine the calcium-binding domain of the protein. (A) Schematic drawing of the recombinant proteins. Six GST-fused recombinant proteins containing the three distinctive domains of tandem repeat 1 ... molecular mass of 64 kDa, and to contain two tandem repeats and a Glu-rich region. The structure of the protein and that of its DNA are similar to those of starmaker, a pro...

Ngày tải lên: 18/02/2014, 17:20