Báo cáo khoa học: Structural basis for substrate recognition by Erwinia chrysanthemi GH30 glucuronoxylanase potx

... 2011 FEBS. No claim to original Slovakian government works Structural basis for substrate recognition by Erwinia chrysanthemi GH30 glucuronoxylanase L ˇ ubica Urba ´ nikova ´ 1 ,Ma ´ ria Vrs ˇ anska ´ 2 , ... struc- ture) for cocrystallization of E. chrysanthemi GH30 xylanase. Fig. S2. Dynamic light scattering profile of E. chry- santhemi GH30 xylanase. Fig. S3. Exa...

Ngày tải lên: 22/03/2014, 16:20

... 905–915. 21 Evdokimov AG, Anderson DE, Routzahn KM & Waugh DS (2001) Structural basis for oligosaccharide recognition by Pyrococcus furiosus maltodextrin-binding protein. J Mol Biol 305, 891–904. 22 ... 274 (2007) 2109–2120 ª 2007 The Authors Journal compilation ª 2007 FEBS Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo⁄ maltodextrin-...

Ngày tải lên: 19/02/2014, 00:20

... 5.2 force field. J Comput Chem 10, 982–1012. Carbohydrate recognition by PAL L. Buts et al. 2420 FEBS Journal 273 (2006) 2407–2420 ª 2006 The Authors Journal compilation ª 2006 FEBS Structural basis ... are fragments of NA2F. The structural information is complemented by thermody- namic parameters of binding determined by means of isothermal titration calorimetry for the above-...

Ngày tải lên: 23/03/2014, 11:20

... of HIV PR with natural cleavage substrate analogs provide new molecular details for understanding the specificity of substrate recognition and a basic framework for the design of new inhibitors ... 946–950. Y. Tie et al. HIV protease complexes with substrate analogs FEBS Journal 272 (2005) 5265–5277 ª 2005 FEBS 5277 Molecular basis for substrate recognition and drug resista...

Ngày tải lên: 30/03/2014, 20:20

... trans- formed strain 300-fold for AZT and 11-fold for ddC when compared to wildtype Dm-dNK. The purified recombinant MuD had increased K m values and decreased k cat values for the four natural substrates but ... decrease in LD 100 for AZT was 300-fold for the double mutant N45D ⁄ N64D and mutant N64D, but only threefold for mutant N45D. Because in human cells AZT is mainly a subs...

Ngày tải lên: 30/03/2014, 20:20

... different substrate- binding mode results from conformational control of the enzyme on the side chain rotamer of the bound substrate. For example, in AsnO, a trans con- former is selected for the ... Collaboratory for Structural Bioinformatics protein data bank accession numbers Crystal structures and structure factors were deposited in the Research Collaboratory for Structural...

Ngày tải lên: 16/03/2014, 02:20

... 878–892. Recognition of Co 2+ by RNA aptamers J. Wrzesinski and S. K. Jo ´ z ´ wiakowski 1662 FEBS Journal 275 (2008) 1651–1662 ª 2008 The Authors Journal compilation ª 2008 FEBS Structural basis for ... Smo ´ lska for her excellent technical assistance. We also thank the reviewers for their comments on the manuscript. This work was sup- ported by grant 6 P04A 081 21, from the...

Ngày tải lên: 16/03/2014, 06:20

... col- oured red for arginine, blue for glutamate and aspartate, green for histidine, cyan for polar, orange for aromatic and aliphatic side chains, brown for Thr77–Gly79 and dark red for Gly153-Asp176. ... the substrate stereoselectivity and catalytic requirements for the hydride trans- fer reaction. A model for substrate recognition and turnover is discussed. The HGDH...

Ngày tải lên: 23/03/2014, 13:20

... 735–740. 11 Fan J, Zhang Q, Tochio H, Li M & Zhang M (2001) Structural basis of diverse sequence-dependent target recognition by the 8 kDa dynein light chain. J Mol Biol 306, 97–108. 12 Wang ... lowest-energy conformers from the selected cluster modelled using the Haddock docking calculations. The rmsd values for the 20 lowest-energy conformers of the best cluster are 0.71 ± 0.1...

Ngày tải lên: 29/03/2014, 09:20

... explana- tion for its poor excision by the enzyme. However, this still did not explain why the catalytic rate (V max ) for U excision in the U 2-hairpin is poor. For productive enzyme substrate complex formation, ... outÕ form. Thereby, U may be spontaneously recognized by UDG. Recently, it has been shown that the excision of U from such hairpin loops by UDG [2,3] is dependent on...

Ngày tải lên: 31/03/2014, 09:20