Báo cáo khoa học: Energetic coupling along an allosteric communication channel drives the binding of Jun-Fos heterodimeric transcription factor to DNA docx

... allosteric communication channel drives the binding of Jun-Fos heterodimeric transcription factor to DNA Kenneth L. Seldeen, Brian J. Deegan, Vikas Bhat, David C. Mikles, Caleb B. McDonald and Amjad ... propagation of information from one monomer to another, which is often the hall- mark of binding of dimeric transcription factors to palindromic DNA...

Ngày tải lên: 22/03/2014, 16:20

... within an acceptable range. This feedback mechan- ism also helps to eliminate cases in which the sys- tem is unable to accurately track the pitch of an utterance. In these cases, the utterance ... recorded, the user rece- ives feedback on the overall quality of the utter- ance. Specifically, the user receives feedback on the pitch, the overall amplitude, and t...

Ngày tải lên: 20/02/2014, 09:20

... complexes [29]. On the other hand, k cat ⁄ K m is an apparent second-order rate constant that refers to the properties and the reaction of the free enzyme and free substrate [29]. We have therefore esti- mated ... than those in the absence of sugars, i.e. K m is decreased. On the other hand, this change in conformation results in a decrease in k cat , the turnover number...

Ngày tải lên: 07/03/2014, 00:20

... VP-ellipsis patterns with other types of anaphora, for instance it can be cataphoric and can locate antecedents from clauses other than the most immediate one. 52 one of two ways. The syntactic VP ... terms of two features: (1) whether the form leaves behind an empty constituent in the syntax, and (2) whether the form is anaphoric in the semantics. In subsequent se...

Ngày tải lên: 08/03/2014, 07:20

... Tyr35 and the heme group in the Phe35fiTyr mutant. The overall structure of the Phe35fiTyr mutant is basically the same as that of the wild-type Tb 5 . The r.m.s. deviation for a total of 82 Ca atoms ... extends into the solvent and does not form any hydrogen bond with the protein atoms. The former propionate displays the conserved conformation in the structures o...

Ngày tải lên: 08/03/2014, 10:20

... general mode of CIII activity, or does it apply only for CII? We examined the mode of the inhibitory action of CIII on proteolysis of the heat shock sigma factor r 32 , another substrate of HflB. Like ... min in the presence of His 6 –CIII or CIIIC (up to 200 lM) are shown in the upper panels. Numbers on the top of each lane indi- cate the concentration of His...

Ngày tải lên: 23/03/2014, 10:20

... Upon densito- metric analysis of the immunoblots it became clear that the amount of CD38 expressed on the plasma membrane and the total amount of CD38 expressed by the various transfectants correlated ... NP-40 (lane 1), Triton X-100 (lanes 2 and 3), digitonin (lanes 4 and 5), Chaps (lanes 6 and 7) or deoxy-BigChap (lanes 8 and 9). Lysates were immunoprecipitated with antibody...

Ngày tải lên: 23/03/2014, 12:20

... types of : , , and that denote the contexts consisting of the noun phrase that the tar- get noun heads, words to the left of the noun phrase, and words to its right, respectively. Then the template ... confidence of ratio is measured by the reciprocal of variance of the ratio (Tanaka, 1977). Variance is calculated by (6) where denotes the number of samples u...

Ngày tải lên: 23/03/2014, 18:20

... h) before the beginning of each experiment. They were then divided into three groups, one maintained at 24 °Candthe other two exposed to cold (6 °C) for either two or 10 days. The fat pads along the ... in the WAT depot and it was therefore not possible to assess the number of these cells and to compare it to that in WT mouse WAT. Genital pads of b 3 KO mice were 1.5...

Ngày tải lên: 23/03/2014, 20:22

... transport in human erythrocytes and so the low affinity transport of DNP-SG and the transport of cGMP may be mediated by one and the same transporter. The character- istics of transport indicate ... were compared using an F-testontheratioofthevariance associated with the reduction in degrees of freedom to the variance of the fit with the smaller number of degrees...

Ngày tải lên: 23/03/2014, 21:20