Báo cáo khoa học: Dynamics in electron transfer protein complexes potx

... favourable orientations of flexible protein domains [43–45], an approach that could also be applied to transient protein complexes. Q. Bashir et al. Dynamics in electron transfer protein complexes FEBS Journal ... Tight-binding inhibition of angiogenin and ribonuclease-A by placen- tal ribonuclease inhibitor. Biochemistry 28, 225–230. 2 Janin J (2000) Kinetics and thermodynam...

Ngày tải lên: 22/03/2014, 16:20

... complexity in the mechanism of electron transfer in this complex flavoprotein. Abbreviations CPR, cytochrome P450 reductase; di-sq, di-semiquinone; ET, electron transfer; hq, hydroquinone; KIE, kinetic ... reflected in its domain organization deter- mined by X-ray crystallography of rat CPR, with the two flavin domains representing independent folding units that are linked by a flexi...

Ngày tải lên: 07/03/2014, 06:20

... and three folded domains: an FAD- and NADPH-binding domain related to ferredoxin-NADP + reductase, a flavo- doxin-like FMN-binding domain and a connecting ÔlinkerÕ domain. In addition to the cytochromes ... reduc- tion in rate of interflavin electron transfer in NADH- reduced CPR thus suggests a role for coenzyme binding, and in particular for interactions made by the 2¢-phosphate o...

Ngày tải lên: 23/03/2014, 17:22

... terminal component of an elec- tron transport chain (Fig. 1) that involves NADH and flavorubredoxin reductase, a FAD-binding protein of the NAD(P)H:rubredoxin oxidoreductase family. The genes coding ... and (b) the electron accepting site in FlRd, the Rd center, is in very fast redox equilibrium with the flavin mononucleotide. Abbreviations eT, electron transfer; FDP, flavodiiron pro...

Ngày tải lên: 16/03/2014, 11:20

Ngày tải lên: 29/03/2014, 08:20

... source of information for effective rec- ommendation. Currently, self-publishing media are becoming increasingly popular. For instance, at this point of writing, Technorati is indexing over 133 ... enriched by such engaging interactions among bloggers and readers as post- ing, commenting and tagging. Prior to this work, the linking structure and user tagging mechanisms in the blogosphere ar...

Ngày tải lên: 30/03/2014, 21:20

... specificity in the protein protein interaction. ETFs are soluble heterodimeric FAD-containing proteins that are found in all kingdoms of life. They contain a second nucleotide-binding site which ... biological electron transfer systems. Abbreviations ACAD, acyl-CoA dehydrogenase; DMButA, n-butyldimethylamine; ETF, electron transferring flavoprotein; ETFQO, electron transferring fla...

Ngày tải lên: 07/03/2014, 05:20

... importance. In biomembranes, electron and proton transfers are often coupled. Determining the kinetics of proton transfers in an individual system is of prime importance in understanding the fine ... order of magni- tude faster in aqueous solution than in the protein environment. This finding is in line with the idea that when partially embedded in the protein, the flavin is l...

Ngày tải lên: 22/03/2014, 21:20

... s, 1 min, 2 min, 3 min, 5 min, 10 min, 15 min and 30 min at room temperature with 200 lL of 100 l M protein. (B) Time course of D ⁄ A ratio for protein- ase K digestion of stFRET. (E) Proteinase ... the filamin A, a-actinin and nonerythrocytic spectrin genes were subcloned into the pEYFP-C1 vector in which the yellow fluorescent protein (YFP) gene was deleted. Different sites in these...

Ngày tải lên: 30/03/2014, 04:20

... similar to that in Asp141 of Met8P. The idea of NirF being a dehydrogenase is appealing because of the presence of a putative nucleotide-bind- ing motif in the N-terminal of the protein sequence and ... consumption in Paracoccus pantotro- phus strains. Starter cultures were grown aerobically in LB with shaking before inocu- lation of mineral salt medium containing 20 m M nitrate in...

Ngày tải lên: 15/02/2014, 01:20