Báo cáo khoa học: Solution parameters modulating DNA binding

Báo cáo khoa học: Solution parameters modulating DNA binding speciﬁcity of the restriction endonuclease EcoRV docx

... 2011) doi:10.1111/j.1742-4658.2011.08198.x The DNA binding stringency of restriction endonucleases is crucial for their proper function. The X-ray structures of the speciﬁc and non-cognate complexes of the restriction nuclease EcoRV ... government works 2727 Solution parameters modulating DNA binding speciﬁcity of the restriction endonuclease EcoRV...

Ngày tải lên : 22/03/2014, 16:20

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Báo cáo khoa học: Functional characterization and Me2+ ion speciﬁcity of a Ca2+–citrate transporter from Enterococcus faecalis1 pdf

... level of the transport- ers themselves. The lack of transport activity of the proline transporter in the presence of Cu 2+ is most likely due to oxidation of the transporter [20]. Poss- ibly, the ... any other deleterious effect on the membrane. Therefore, the lack of transport of citrate by the membranes con- taining EfCitH and of proline by all membranes in...

Ngày tải lên : 23/03/2014, 10:20

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Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... mice [28]. The instability of DA at the neutral pH of the cytosol causes the formation of toxic aminochromes, and these species are not formed when DA is protonated at the acidic pH of the storage ... vesicle binding. Co-expression of p25a and the dopamine transporter in HEK-293-MSR cells sensitized them to the toxicity of extracellular dopamine. Neuronal expres...

Ngày tải lên : 14/02/2014, 21:20

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Tài liệu Báo cáo khoa học: Mixed lineage leukemia: a structure–function perspective of the MLL1 protein ppt

... include the MLL1 CXXC domain [22], a portion of the MLL1 TAD bound to the KIX domain of the cAMP response element -binding (CREB) binding protein (CBP) [23], a peptide from the Win motif of MLL1 bound ... value as inhibitors of SET1 family complexes. Binding of the MLL1 Win motif to the central argi- nine -binding pocket of WDR5 raises questions about the pro...

Ngày tải lên : 16/02/2014, 14:20

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Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf

... in all of these conditions [22]. In the trau- matically injured brain, however, the synthesis of MT1 and MT2, as well as the synthesis of GIF, are up-regu- lated by reactive astrocytes in the vicinity ... ﬁne processes of these cells. Astrocytic synthesis of GIF is mainly found in the cortex, brainstem and spinal cord [6]. Additionally however, there are also consistent re...

Ngày tải lên : 16/02/2014, 15:20

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Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx

... [6] and inhibits the tyrosine kinase activity of the IR [7]. On the basis of the crystal structure of the tyrosine kinase domain of the IR in complex with the PIR ⁄ BPS domain of Grb14, Depetris ... we cannot assess whether the kinase activity of the IRs before KCl treatment repre- sents residual activity of receptors with Grb14 bound to them, the activity o...

Ngày tải lên : 18/02/2014, 18:20

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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf

... described in the Materials and methods. Completion of the hydrolysis was conﬁrmed by the disappearance of the (Met1)-ONC (M23L) signal in the MALDI-TOF mass spectra 1 h after the addition of AAP. The ... calculating the slope of the curves of Pyr-ONC (M23L) vs. time. From the rates of cyclizationshowninTable1,itcanbeinferredthatthe formation of the N-termin...

Ngày tải lên : 19/02/2014, 12:20

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Tài liệu Báo cáo khoa học: "Phrase-Based Backoff Models for Machine Translation of Highly Inﬂected Languages" docx

... order to study the effect of varying amounts of training data we created several training partitions consist- ing of random selections of a subset of the full training set. The sizes of the partitions ... hope that the united nations in the negotiations to reach a conclusion that the greek and turkish accession to the beneﬁ t of the beneﬁ ts of the republic...

Ngày tải lên : 22/02/2014, 02:20

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Báo cáo khoa học: CD91 interacts with mannan-binding lectin (MBL) through the MBL-associated serine protease-binding site doc

... effect (Fig. 4C). In the case of L-ﬁcolin, the interaction with CD91 was abolished in the presence of EDTA, but the presence or absence of Ca 2+ ions in the buffer had no effect on the interaction ... and kinetics. The CD91 used contains residual attached RAP, which inﬂuences the concentration of active CD91 and therefore the kinetics of the interaction when...

Ngày tải lên : 06/03/2014, 01:23

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Báo cáo khoa học: Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger docx

... and in the range of the CV of the NADPH determination. Discussion Obvious tendencies found by characterization of the wild-type In the exponential phase of the ammonium cultures all of the PPP ... [26]. Overproduction of 6PGDH resulted in an increase of synthesis of pyr- idine nucleotide cofactors, as the total pool of these increased. Whether the synthesis...

Ngày tải lên : 07/03/2014, 17:20

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