Báo cáo khoa học: Enzymatic actions of Pasteurella multocida

Báo cáo khoa học: Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated a subunit of the heterotrimeric GTPase Gq potx

... actions of Pasteurella multocida toxin detected by monoclonal antibodies recognizing the deamidated a subunit of the heterotrimeric GTPase G q Shigeki Kamitani 1 , Shinpei Ao 2 , Hirono Toshima 1 , ... Ga q was also considered to be deamidated by the toxin. The deamidated GTPases were found to lose their GTPase activity and, as a result, stim...

Ngày tải lên : 22/03/2014, 16:20

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Báo cáo khoa học: Gene expression silencing with ‘speciﬁc’ small interfering RNA goes beyond speciﬁcity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx

... 5¢- UGACUUCCCUGGCCUAUUUUU-3¢,5¢-ACAUUGAGC UCCUCUCUUGUU-3¢,5¢-GAUAAGGUUGCUUCAGU UAUU-3¢ and 5¢-ACAGUACGCUAUGAAACUAUU-3¢, respectively. As a negative control, we used an NT siRNA (5¢-UAGCGACUAAACACAUCAA-3¢) or a ... (forward, 5¢-GGCCCAG GTGACTCAGCTATT-3¢; reverse, 5¢-AGGGCATCCGA GAATTCCTT-3¢), LAMP2 (forward, 5¢-TCAGCATTGC AAATAACAATCTCA-3¢; reverse, 5¢-CAGTCTGCTCT TTGTTGCACATATAA-3¢), CTGF...

Ngày tải lên : 07/03/2014, 05:20

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Báo cáo khoa học: Recombinant bovine zona pellucida glycoproteins ZP3 and ZP4 coexpressed in Sf9 cells form a sperm-binding active hetero-complex ppt

... Noguchi S, Yonezawa N, Katsumata T, Hashizume K, Kuwayama M, Hamano S, Watanabe S & Nakano M (1994) Characterization of the zona pellucida glycoproteins from bovine ovarian and fertilized ... Amari S, Yonezawa N, Mitsui S, Katsumata T, Hama- no S, Kuwayama M, Hashimoto Y, Suzuki A, Takeda Y & Nakano M (2001) Essential role of the nonreduc- ing terminal a- mannosyl residues...

Ngày tải lên : 07/03/2014, 05:20

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Tài liệu Báo cáo khoa học: Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate: ferredoxin oxidoreductase from Hydrogenobacter thermophilus doc

... Ozawa Y, Nakamura T, Kamata N, Yasujima D, Urushiyama A, Yamakura F, Ohmori D & Imai T (2005) Thermococcus profundus 2-ketoisovalerate ferredoxin oxidoreductase, a key enzyme in the archaeal energy-producing ... to the oxidative decarboxylation catalyzed by this enzyme. (2) The nucleophilic TPP C2-carbanion attacks the carbonyl carbon of acetyl-CoA (as it attacks the car-...

Ngày tải lên : 16/02/2014, 09:20

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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx

... 5¢-CAGCCGCCGTAGAA GTT GTAAGTCATCGCAAAG-3¢,5¢-CGCCGCCACCAGGG AA CATCCAGTCAATATCTAC-3, and 5¢-GCCGCCAC CAGGGAA TTGCCAGTCAATATCTAC-3¢, respectively. Conﬁrmation of the mutated nucleotides by automated sequencing was carried ... In the case of the E315Q mutant, an additional faint band was also seen at an M r of  43 000. This band appeared as a degradation product during freezing...

Ngày tải lên : 20/02/2014, 01:20

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Báo cáo khoa học: Enzymatic investigation of the Staphylococcus aureus type I signal peptidase SpsB – implications for the search for novel antibiotics ppt

... TA CATATGCACCATCACCATCACCATAAAAAAGAATTATTGGAATGGATTATTTC NdeI ﬂ-SpsB3 TA GAATTCTTAATTTTTAGTATTTTCAGG EcoRI tr-SpsB5 TA CATATGCACCATCACCATCACCATATTGTTACACCATATA NdeI pIsaA5 TA CCATGGCACATCACCATCACCATCACAAAAAGACAATTATGGC ... TA CCATGGCACATCACCATCACCATCACAAAAAGACAATTATGGC NcoI IsaA3Myc TA GAATTCTTACAGATCCTCCTCTGAGATGAGCTTCTGCTCGAATCCCCAAGCACCTAAACC EcoRI Rao C. V. S. et al. S. aureus type I sign...

Ngày tải lên : 07/03/2014, 01:20

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Báo cáo khoa học: Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis ppt

... changing the target of the physiological attack [138]. The acidic component of vipoxin is a nat- ural inhibitor of the basic and catalytically active PLA 2 . In the absence of the PLA 2 -like ... non-enzymatic subunit has a heavy chain (consisting of A1 and A2 domains) and a light chain (consisting of A3 , C1 and C2 domains) that are held together by non...

Ngày tải lên : 14/03/2014, 22:20

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Báo cáo khoa học: Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal b-N-acetylhexosaminidase pot

... moved by more than 0.3 nm. Most (83.5%) of the amino acid residues are plotted in the favourable regions of the Ramachandran plot. The deviation of geometrical parameters from ideal values (G-factors) ... incorporates a consistency score to evaluate alignment. A structural alignment of the three templates was additionally made with the sheba plug-in and visualized...

Ngày tải lên : 14/03/2014, 23:20

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Báo cáo khoa học: Enzymatic features of the glucose metabolism in tumor cells ppt

... CoA UTPPP ATP ADP ATP ADP NAD NADH 2 ADP A TP ADP NADNADH2 NADH2NAD NADPH2NADP NADP ATP AMP ADP ADP NADPH2NADP GLUT1 HK2 PFKFB3 PGM-M PK-M LDH -A MCT4 PDHK-1 TKTL1 TALD1 TKTL1 BGP ISOFORM Isoform change Expression ... Plasma membrane lactate transport (LACT) Lactate is transported over the plasma membrane by facilitated diffusion either by the family of proton-linked Tumor speciﬁ...

Ngày tải lên : 14/03/2014, 23:20

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Báo cáo khoa học: Enzymatic oxidation of NADP+ to its 4-oxo derivative is a side-reaction displayed only by the adrenodoxin reductase type of ferredoxin-NADP+ reductases potx

... aliquots were withdrawn, AMP was added as internal standard, and sam- ples were analyzed by ion exchange chromatography as des- cribed above. The amount of NADPO was determined on the basis of ... coefﬁ- cient of the NADP derivative at 260 and 254 nm, respectively, as calculated by determining the concen- tration of the nucleotide on the basis of the phosphate rele...

Ngày tải lên : 16/03/2014, 11:20

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