Báo cáo khoa học: Structural basis of p63a SAM domain mutants involved in AEC syndrome ppt

... of the SAM domain family. SAM domains, in general, were initially thought to be involved in pro- tein–protein interactions by forming homo- or hetero- oligomers with other SAM domains or by interacting with ... representation of the p63a SAM domain showing the position of mutations that are associated with AEC syndrome. Table 3. Thermodynamic values of p63a...

Ngày tải lên: 22/03/2014, 16:20

... formation of the electron rich hydrochinone form. Furthermore, p-stacking interactions play a role in protein binding of flavins. For example, in the recently discovered flavoprotein dodecin, a pair of ... of flavins in a protein environment. In order to p rovide the structural basis for further studies of the physical properties of riboflavin in complex with lumazine sy...

Ngày tải lên: 30/03/2014, 15:20

... functional domains and motifs, including a PB1 domain in the N-terminus, a pseudosubstrate (PS) sequence, a C1 domain of a single Cys-rich zinc-finger motif, and a kinase domain in the C-terminus. ... de Murcia, Spain Protein kinase C (PKC) is a family of related protein kinases that plays an important role in regulating cell growth. These protein kinases are involved in se...

Ngày tải lên: 16/03/2014, 13:20

... be involved in regu- lating other functional domains of Mxi1 (e.g. the SID or the bHLH ⁄ LZ) and ⁄ or in the recruitment of other activ- ities. However, in some assays, the presence of this domain ... the isoforms carried Myc tags on their COOH termini. SID, Sin3 interacting domain; BR, basic region; HLH, helix-loop-helix; CT, carboxyl termi- nus. (A, lower) Western blotting...

Ngày tải lên: 18/02/2014, 16:20

... sugar- binding pocket of At1g21360 very rich in arginines. Fourthly, the residue corresponding to Val209 in human GLTP corresponds to Ser202 in AtGLTP1, a methionine in At1g21360 and a proline in ... filaments of 6-week-old plants. In (F), GUS staining appeared to be more intense in stomatal cells, and staining was most evident in distal regions of floral organs (G–I). In...

Ngày tải lên: 23/03/2014, 07:20

... 2737 Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain Natsuko ... less than one-tenth of the K cat ⁄ K m value of the soluble form of the wild-type enzyme, indicating that the replacement of valine with alanine at positi...

Ngày tải lên: 07/03/2014, 05:20

... substrate l-arginine, but also tolerates l-homoarginine and l-canavanine (Fig. 3A, Table 1). These findings can be explained by the coor- dination of l-arginine in the active site of VioC (Figs ... methylene group in the side chain of l-arginine, as exemplified by the binding and catalytic turnover of l-homoarginine. Concerning l-canavanine turnover by VioC, the modified guanidinium...

Ngày tải lên: 16/03/2014, 02:20

... spectrum. Therefore, in order to gain a glo- bal insight into metal ion–RNA interactions, it is often necessary to conduct structural studies of metal ion binding modes in RNA molecules using biophysical methods ... Co 2 binding site in selected aptamers and their involvement in the metal ion coordination (Fig. 4). Participation of the N7 atom of purines in the organization...

Ngày tải lên: 16/03/2014, 06:20

... working protein volume of 1.3 mL under constant stirring at 350 rpm. Experimental data were fitted using Microcal’s origin 5.0 software package to obtain the binding constant K b and binding enthalpy ... PAL. Stereo view of the binding of M592 (green) to the )1 and M subsites of con A (colored according to atom type). The corresponding situation in PAL is superimposed as shown in t...

Ngày tải lên: 23/03/2014, 11:20

... protein util- izing the principle of protein-dye binding. Anal Biochem 72, 248–254. 30 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature ... were transformed into KY895 and then the strains were examined for growth, +, in the presence of different concentrations of thymidine in the medium. In the last column, LD 1...

Ngày tải lên: 30/03/2014, 20:20