Báo cáo khoa học: Efficient ATP synthesis by thermophilic Bacillus FoF1-ATP synthase ppt

... Efficient ATP synthesis by thermophilic Bacillus F o F 1 -ATP synthase Naoki Soga 1 , Kazuhiko Kinosita Jr 1 , Masasuke Yoshida 2,3 and ... equipped with a sample injection apparatus. The synthesis reaction was driven by acid–base transition and valinomycin-medi- ATP synthesis by thermophilic ATP synthase N. Soga et al. 2652 FEBS Journal ... TF o F 1 , Bacillus...

Ngày tải lên: 22/03/2014, 16:20

... ligation by reverse-joined hairpin ribozymes Reverse-joined hairpin ribozymes, first introduced by Ohtsuka and colleagues [29,30], are derived from the conventional hairpin ribozyme by dissecting ... ⁄ HCl (pH 7.5) buffer was heated at 90 °C for 1 min followed by incuba- tion at 37 °C for 15 min. The cleavage reaction was started by addition of MgCl 2 and spermine. The final volume of...

Ngày tải lên: 20/02/2014, 01:20

... matrix, with p = 0.5. 3 The use of such sparse projections was first proposed by Achlioptas (2003), then extended by Li et al. (2006). 3 Note that if using the pooling trick of Van Durme and Lall (2010), ... extended by Vitter (1985) to allow for multiple updates. Reservoir Counting If we are sampling over a stream drawn from just two values, we can implic- itly represent the reservoir...

Ngày tải lên: 20/02/2014, 04:20

... T Σ and v ∈ pos(t). The label of t at position v, denoted by t(v), the subtree of t at v, denoted by t| v , and the replacement at v by s, denoted by t[s] v , are defined as follows: 1. For every σ ∈ ... derivation step from s to t by replacing some leaf of s labeled with q and a tree matching y by a transformation of u, where each instance of a vari- able has been replaced by a...

Ngày tải lên: 17/03/2014, 00:20

... chloroform/water. These mRNAs were purified by gel filtration with NICK columns and finally by ethanol precipitation. Cell-free translation The wheat-germ extract was prepared by the reported method [1]. DTT ... without a radioisotope and purified by the above method. The resulting solution (50 lL) was neutralized by 200 m M phosphate-buffered Tween 20 (PBT), pH 8.0, desalted by G25 s...

Ngày tải lên: 16/03/2014, 23:20

... and found that SW480 cells were efficiently killed by the hpST3dODN. SW480 cells were also efficiently killed by IFN-c treatment, and this action was counteracted by hpST3dODN, which reduced transcriptional ... assays. However, the same cells were efficiently killed by interferon-c. This effect was counter- acted by the STAT3 oligodeoxynucleotide, which was found to efficiently inhibit...

Ngày tải lên: 18/02/2014, 08:20

... for efficient DNA and small interfering RNA delivery in vitro and in vivo. Cancer Gene Ther 12, 321–328. 72 Lia S-D, Chono S & Huang L (2005) Efficient gene silencing in metastatic tumor by ... gene regulation by RNAi. Nat Biotechnol 21, 635–637. 14 Fedorov Y, Anderson EM, Birmingham A, Reynolds A, Karpilow J, Robinson K, Leake D, Marshall WS & Khvorova A (2006) Off-target effec...

Ngày tải lên: 06/03/2014, 01:23

... “half-constituent” headed by h, whereas an incomplete span is only a partial half-constituent, since the constituent can be extended by adding more modifiers to m. Each type of span is created by recursively combining ... limited interactions. By exploiting the additional constraints arising from the factorization, maximizations or summations over the set of possible dependency trees c...

Ngày tải lên: 07/03/2014, 22:20

... past studies, it is not always efficient. The computational cost of the Viterbi algorithm is O(NL 2 ), where N is the input length and L is the number of labels; it is efficient enough if L is small. ... label se- quence by iteratively solving labeling problems with a reduced label set. This results in signifi- cant time savings in practice, because each itera- tion becomes much more effic...

Ngày tải lên: 07/03/2014, 22:20

... error rate drops by more than half. This is further supported by John- son (2001), who saw no parsing gains when switch- 959 ing from generative to discriminative training, and by Petrov et al. ... form. We have a feature function, represented by f(r,s), which returns a vector with the value for each feature. We denote the value of feature f i by f i (r,s) and our model has a corres...

Ngày tải lên: 08/03/2014, 01:20