Báo cáo khoa học: Membrane protein integration into the endoplasmic reticulum doc

... to the translocon. The tran- slocon, a multiprotein complex, facilitates the insertion of integral membrane proteins into the lipid bilayer [3] and the translocation of soluble proteins into the ... integral membrane proteins in eukaryotic cells occur cotranslationally, whereby protein synthesis and integration into the endoplasmic reticulum (ER) membrane are...

Ngày tải lên: 22/03/2014, 15:21

... in the luminal loop between their two membrane- span- ning segments. The N-terminal domain is then released from the membrane by S2P, a membrane- bound zinc metalloproteinase. In the cytoplasm, the ... transcriptional factors. Protein interaction on the ER and in the cytosol SREBPs are localized on the ER membrane, associat- ing with another ER membrane protein, SREB...

Ngày tải lên: 18/02/2014, 13:20

... with the word “poor.” The method used to derive these values takes advantage of the possibility of using the World Wide Web as a corpus, similarly to work such as (Keller and Lapata, 2003). The ... derived by measuring the relative minimal path length (MPL) in WordNet between the adjective in question and the pair of words appropriate for the given factor. In the case of...

Ngày tải lên: 31/03/2014, 03:20

... then treated with T4 DNA polymerase and T4 DNA ligase to fill the gap between the cDNA and the tag. 6-Carboxyl-fluorescein was removed by DpnII digestion. Then the antisense strand of cDNA on the microbeads ... trea- ted with the SDS ⁄ PAGE buffer. Twenty-five micrograms of protein was subjected to SDS ⁄ PAGE and then blotted onto a poly(vinylidene difluoride) membrane. The At SE...

Ngày tải lên: 07/03/2014, 21:20

... divides the linguis- tic meaning of the sentence into parts that link the content to the context, and others that advance the discourse, i.e. add or modify information; and (ii)  We use the Praguian ... summarized in Table 1. These instructions are based on the surface word order, the position of the sentence stress (intonation center – IC 3 ) and the canonical order of...

Ngày tải lên: 23/03/2014, 19:20

... supporting the existence of a protease that cleaves s-DAPK-1 protein in vivo. The reason why the cleavage band was not observed in this assay may be the rapid degradation of the purified protein from the cell ... is considered to be the source of the contractile force underlying the bleb formation [11]. Furthermore, microtubule-associated protein 1B interaction with th...

Ngày tải lên: 18/02/2014, 17:20

... of the membrane proteins of Leishmania which were surface iodinated. LBP is an integral membrane protein The hydrophobic nature of LBP was confirmed by recon- stituting the purified protein into ... oxidase. Membrane proteins were then isolated by biotinylation and streptavidin/agarose extraction, analysed by SDS/PAGE and autoradiographed. The 67-kDa LBP, together with other...

Ngày tải lên: 21/02/2014, 00:20

... novel member of the MPP5 protein scaffold at the outer limiting membrane (OLM), and of the DLG1 protein scaffold at the outer plexiform layer of the retina. MPP3 localized at the photoreceptor ... photoreceptors and Mu ¨ ller glia cells. Based on the recruitment of MPP3 to the MPP5 protein scaffold at the OLM, the involvement of MPP5 in the CRB1 protein scaffol...

Ngày tải lên: 16/03/2014, 13:20

... at the egg [22]. These authors did not determine the source of the enzyme responsible for the cleavage of VE protein in the ovary; however, they speculated that the enzyme is associated with the ... pos- sesses the ability to reconstruct the PL of Japanese quail. To achieve this, we purified ZP1 proteinfrom the serum and from the PL lysate and then injected the protei...

Ngày tải lên: 23/03/2014, 07:20

... that the signal peptide is required for the targeting process (Fig. 5A). Next, to probe whether the ATP-dependent targeting of preHoloHiPIP to the membrane required the twin arginine motif in the ... with the salt-wash-resistant association of the precursor with the membranes suggests a membrane- insertion process. As the C-terminus of preHoloHiPIP binds the cofactor...

Ngày tải lên: 23/03/2014, 21:20