Báo cáo khoa học: Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida pptx

... molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida Brenda A. Wilson and Mengfei Ho Department of Microbiology and Host–Microbe Systems Theme of the Institute ... 2011) doi:10.1111/j.1742-4658.2011.08158.x The mitogenic toxin from Pasteurella multocida (PMT) is a member of the dermonecrotic toxin family, which...

Ngày tải lên: 22/03/2014, 15:21

... A3(2). Cloning and expression of the putative hyd genes The deduced open reading frames of putative hyd genes were PCR-amplified from the genomic DNA of the corresponding organisms. The PCR fragments of the ... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode o...

Ngày tải lên: 18/02/2014, 08:20

... properties of both conserved and unique components of the Cag-T4SS, together with potential implications for the current understanding of its mode of action. The cagPAI and the Cag type IV secretion ... to other systems, both with respect to the composition of its secre- tion apparatus and the molecular details of its secretion mechanisms. This review describe...

Ngày tải lên: 06/03/2014, 00:21

... products of the hazelnut LOX were compared with the reaction products of soybean LOX type I and authentic standards of 9- and 13-HODE. As shown in Fig. 4, 9-HODE is the main product of the hazelnut ... providing the substrates for the activity of all the enzymes located downstream on the pathway, have a primary role in the biosynthesis of phytooxylipins and...

Ngày tải lên: 21/02/2014, 00:20

... only one band was amplified from each of the different samples and Southern blot analysis using the probe prepared with the lac1 primers also revealed a single band. Further- more, all of these generated ... positively affected the activity and stability of the enzyme [40]. The effect of copper on enzyme stability may be related to the inhibitory effect of the m...

Ngày tải lên: 07/03/2014, 15:20

... features of the sPLA 2 s are well conserved: the central a-helices with the catalytic His–Asp dyad, and the hydrogen- bonding network connecting the interfacial binding site, the catalytic site and the ... of the hydrolysis products [12,19]. In order to investigate the effect of bile salts on ChPLA 2 activity, the rate of hydrolysis of PC by ChPLA 2 in the...

Ngày tải lên: 30/03/2014, 01:20

... II. The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig. 1A). The two ... atoms, whereas the side chain of Lys173 interacts with both O6 and N7 (Fig. 1C). The 2¢OH of the ribose is hydro- gen bonded to the main chain and the...

Ngày tải lên: 15/02/2014, 01:20

... orientation of the N5 of the flavin, the hydride to be transferred, and C4 of the nicotinamide. In FNR, displacement of the C-terminal Tyr appears to be required for the interaction to occur [117,118]. The ... because the semiquinone H-bond with the CO of Asn is weaker than the bond formed with the sma- ller Gly, and because of the presence in the oxid...

Ngày tải lên: 18/02/2014, 11:20

... at a scanning rate of 50 nm Æmin )1 with a time constant of 2 s, step resolution of 0.1 nm, and bandwidth of 1 nm. The final spectra were taken from the average of five s cans. The V ARSELEC program ... excess of 3 for IFP–wt) and the shape o f I x /x vs. x plot. Specifically, the initial slow rise of the l atter plot (from x ¼ 1) reflects that the self-associat...

Ngày tải lên: 19/02/2014, 16:20

... [57]. Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the complementary strand of the nucleotide sequence from 89 254 to 89 667 in the Synechocystis ... was observed. This band was also observed in fractions from the other purification steps. The band was cut out from the gel followed by elution of the...

Ngày tải lên: 19/02/2014, 17:20