... room temperature under field of 5 kOe and this measurement with the increase of Co 2+ concentration yields the monotonic increase of saturation magnetization (M S ) and coercive field (H C ). ... moment per formula of Ni 1-x Co x Fe 2 O 4 and equivalently, an enhancement of magnetization [7].The dependence of saturation magnetization M s as a function of Co 2+ co...
Ngày tải lên: 13/02/2014, 04:20
... inhibition of ITK activity compromises HIV infection, gene expression and replication [62]. Our group has recently evaluated the effect of proteasome inhibitors on HIV infection and ⁄ or replica- tion. ... Authors Journal compilation ª 2011 FEBS MINIREVIEW TEC family kinases in health and disease – loss -of- function of BTK and ITK and the gain -of- function fusions ITK–SY...
Ngày tải lên: 14/02/2014, 18:20
Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
... Apparent concentrations ratio (cell/medium) Dye concentration (µM) On ice + CCCP Fig. 2. Effect of rhodamine dye concentrations on their uptake into K562 cells. K562 cells were incubated either on ... fluorescence was monitored continuously with an excitation wavelength of 555 nm and an emission wavelength of 575 nm. Inhibition of the NBD-Cl-sensitive efflux allowed the upt...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... residue, and absorption of tyrosine at 275 nm (e 275 = 1400 m )1 Æcm )1 ) was used to estimate the concentration of Ab in solution. In four separate puri- fication experiments, the concentration of ... E22K, E22Q and D23N), and the availability of a rapid and simple expression and purification protocol will facilitate large-scale investigations of the molecular determina...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... mRNA and its own transcript. Overexpression of MCPIP in cells activated with LPS results in downregulation of the IL-1b transcript and silencing of MCPIP gene expression has an opposite effect on ... fast degradation of IL-1b transcript independently of the presence of AREs (Fig. 6B, lanes 2 and 5). It is possible that in the region located before the AREs, consisting o...
Ngày tải lên: 18/02/2014, 14:20
Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx
... a thorough understanding of not only their structures, but also of the energetics of their folding reactions and conformational transitions among different forms. The structure of human telomeric ... strand, allowing for the calculation of the equilibrium distribution of conformational forms. Standard methods using UV absorbance at a single wavelength to monitor the thermal dena...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... Materials and methods. Relative band intensities were recorded using a CCD camera, and the concentrations of individual variants was calculated from a calibration curve of known 44/750 concentrations. ... chro- matography and chromatofocusing, as described previously [21]. Results Expression and secretion of truncated variants of GCPII To analyze the contribution of individu...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identified by site-directed mutagenesis pot
... mm NaCl. Effects of CL1 and CL2 site mutations on the chloride sensitivity of tACE and ACE2 In order to observe the effects of the various mutations on the chloride sensitivity of tACE and ACE2, ... result of substitution of the tACE residues Pro407 and Pro519 with Glu398 and Ser511, and the resulting projection of their side-chains into the location of this si...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris ppt
... After elution and concentration of the protein solution containing the cellulase activity after native PAGE, the solution was mixed with sample buffer for SDS/ PAGE without reducing agent and incubated ... hilaris cellulase contained an ORF 975 bp long, starting with an ATG codon at position 38 and ending with a TAA codon at position 1014. A poly(A) tail and typical polyadenylatio...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
... the protein at high concentrations. The present study describes a very effective method of isolation and purification of PEC and its photoactive a-subunit. Crystallization and preliminary X-ray ... improving purification protocols. As a result, several comparative studies on the Z-andE-configurations of the intact a-subunit, and also on photoactive peptides originating from non...
Ngày tải lên: 17/03/2014, 10:20