Atlas of Human Pluripotent Stem Cells doc

... Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder Layer-Free Conditions 41 3.1 Introduction 41 3.2 Materials for Feeder Layer-Free Culture of hPSCs ... Abstract Human embryonic stem cells (hESCs) are pluripotent cells derived from the inner cell mass (ICM) of the developing embryo. They have tremendous poten- tial fo...

Ngày tải lên: 22/03/2014, 09:20

... and power failure. Key words: human embryonic, stem cells, low temperature 1. Introduction In vitro culture of human embryonic stem cells (hESC) often involves temporary exposure to reduced ... Published: 2006.07.25 This study investigated the effects of exposing human embryonic stem cells (hESC) to 4 o C and 25 o C for extended durations of 24h and 48h respective...

Ngày tải lên: 31/10/2012, 16:57

... murine ES cells and in human hematopoietic progenitor cells. Discussion The commitment of pluripotent stem cells to succes- sively less plastic progenitors and, finally, differentiated cells exhibiting ... developmental progres- sion of cell samples and in all cases except that of the Rex-1 gene, primer sets span introns. Day 0 cells are composed of ES cells and MEF...

Ngày tải lên: 07/03/2014, 12:20

... none of those considered (i.e. size of the tumor, degree of differ- entiation, and the presence of pseudocapsule invasion) was related to miR-23b expression in HCC. 3¢-UTR constructs Segments of ... correct orientation of the insert was verified by sequencing. Transient transfection of SKHep1C3 cells with miR-23b and transient transfection of AB2 cells with anti-miR-23b Mol...

Ngày tải lên: 29/03/2014, 23:20

... specific features of the plasticity of adult stem cells are largely unknown. Recently, we demonstrated the hepatic differentiation of human adipose tissue-derived mesenchymal stem cells (AT-MSCs). ... GM, Claas FH, Fibbe WE & Kanhai HH (2004) Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta. Stem Cells 22, 1338–1345. 6 Biebac...

Ngày tải lên: 30/03/2014, 04:20

... produced upon treatment of cells with MTX, using cDNA arrays that allow the simultaneous evaluation of expression, at the mRNA level, of hundreds of genes. Specifically, the Atlas Human Cancer 1.2K ... One microgram of pcDNA-VIM or 0.5 lM aODN-VIM were lipofected either in the absence or in the presence of 10 )8 M of MTX in parental HT29 cells (A) or 10 )5 M of MTX in H...

Ngày tải lên: 07/03/2014, 16:20

... effect of Pycnoge- nol Ò on pure TrxR1 enzyme activity (data not shown). Effects on the viability of U1285 cells The toxicity of the compounds used was assessed by means of determination of the ... selenium supplementation [31], probably due to the higher ranking of TrxR1 in terms of the hierarchy of selenoenzymes [32]. Treatment of cells with an organic selenium compoun...

Ngày tải lên: 30/03/2014, 02:20

... Understanding of molecular mechanisms governing the enucleating phenomena of human erythrocytes is of major importance in both fundamental and applied studies. Total RNA (n=7) from human RBCs (purity of ... 1). Table 1 The purity of human RBCs fraction tested by independent methods. blood cell count (n=5) RBCs (cells/ µL) white blood cells (cells/ µL) platelets...

Ngày tải lên: 03/11/2012, 10:58

... Dose Amount excreted per unit of time [amount/t] Notional plasma volume per unit of time freed of drug = clearance [vol/t] Unit of time Time Co Lüllmann, Color Atlas of Pharmacology © 2000 Thieme All ... concentra- tions of about 0.02 %, and the amount of drug eliminated per unit of time re- mains constant at concentrations above this level. 44 Pharmacokinetics L llmann, Col...

Ngày tải lên: 26/01/2014, 19:20

... years. They see life in terms of ending and the younger version of immortality is not a part of their vision. Also, life events can change their view of the importance of dietary adherence in a positive ... Strategies Methods of Tailoring Modeling Model preparation of quick, four-ingredient dessert parfait Self-Monitoring Include two simple forms of self-monitoring that all...

Ngày tải lên: 15/02/2014, 16:20