... andE in greater detail, the single ecIII cysteine mutants,ecIIIA398C, ecIIIS404C, ecIIII406C, ecIIIG408C,ecIIIM409C, ecIIIV411C (loop D), and ecIIIY431C (loopE), were expressed in the cysteine-free ... determined for the mutants generated in this investigation i.e. ecIIIA398C, ecIIIS404C, ecIIII406C,ecIIIG408C, ecIIIM409C, ecIIIV411C and ecIIIY431C(Table 1).Fig. 3. NADP(H) binding region of dIII ... cysteinemutants ecIIIA398C, ecIIIS404C, ecIIII406C, ecIIIG408C,ecIIIM409C, ecIIIV411C and ecIIIY431C. In addition,four previously produced mutants, i.e. ecIIID392C [15],and ecIIIT393C, ecIIIG430C...