... these datamay be of particular importance in understanding the physiological role of b-glycosidases and in designinginhibitors. In addition, another important issue in understand-ing the aglycone ... that interact directly with the aglycone (basalplatform and ceiling) [15 ]. Nevertheless, the relativecontributions of the aglycone-binding residues in the substrate binding and catalysis are ... mutagenesis, generating the mutantsE19 0A, E190Q, E19 4A, K20 1A, K201F and M45 3A. Replacements with A were made to remove side chainsthat could interact with the aglycone. MutationsE190Q and...