... identity to PGDH from B. thetaiotaomicron, E. caudatum, B. fra- gilis and P. gingivalis, 35% to Methanococcus jannaschii, 33% to Archaeoglobus fulgidus and Thermoanaerobacter tergcongensis,31%toBacillus ... PGDH. The pea PGDH activity was stabilized in the presence of 2.5 M glycerol or 100 m M 2-mercaptoethanol [9]. Similarly, when rEhPGDH was stored in 50 m M Tris/HCl buffer, pH 8.0 containi...
Ngày tải lên: 19/02/2014, 13:20
Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx
... mL 0.1 m sodium phosphate, pH 7.0, containing 0.1 mm ThDP ⁄ MgSO 4 ,5%v⁄ v glycerol, 1 mm phenylmethanesulfo- nyl fluoride, 1 mm dithiothreitol (DTT) and 1 mm EDTA, and disrupted using a French ... kinetic analysis of the decarboxylation of pyruvate by 3,4-dimethylthiazolium ion in water and ethanol. J Am Chem Soc 92, 5707– 5716. 23 Bradford MM (1976) A rapid and sensitive method for the
Ngày tải lên: 06/03/2014, 11:20
The Hero of Ticonderoga or Ethan Allen and his Green Mountain Boys docx
... Ira, came in, and threw himself on the settee in the chimney corner opposite Ethan. Ira was thirteen years younger than Ethan, but as bold and daring as his brother. At the date of the formation ... IV. "THE RISING OF THE MOON." Eben Pike burst open the door of Ethan Allen's house without any ceremony. Ethan and Seth Warner were sitting on the settee in the chimney, talk...
Ngày tải lên: 17/03/2014, 23:20