The a and b adapters are used as priming sites for both amplification
The a and b adapters are used as priming sites for both amplification
... B adapters are ligated to the < /b> blunt ends using DNA ligase • The < /b> strands are denatured using sodium hydroxide to release the < /b> ssDNA template library (sstDNA). The < /b> Adapters • The < /b> A < /b> and < /b> B adapters ... ssDNA bead to be loaded into a < /b> well. • Enzyme beads and < /b> packing beads are added. Enzyme beads containing sulfuras...
Ngày tải lên: 19/03/2014, 22:32
Báo cáo khoa học: Expressed as the sole Hsp90 of yeast, the a and b isoforms of human Hsp90 differ with regard to their capacities for activation of certain client proteins, whereas only Hsp90b generates sensitivity to the Hsp90 inhibitor radicicol pdf
... extracellular signal-regulated kinase-5 (ERK5) mitogen-activated protein (MAP) kinase [18]. GR assays indicated that human Hsp9 0a < /b> and < /b> Hsp9 0b, as well as the < /b> native yeast Hsp90s, were all capable ... mammalian clients are able to be activated by both Hsp9 0a < /b> and < /b> Hsp9 0b. Whether Hsp9 0a < /b> or Hsp9 0b is expressed in the < /b> yeast, however,...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces significant structural changes doc
... and < /b> four Cu(I) loaded a-< /b> and < /b> b- domains, no interaction was seen between the < /b> two species. There was neither any indication for a < /b> net transfer of Cu(I) between the < /b> two domains nor for the < /b> formation ... vivo, and < /b> are believed to be important for d 10 -metal control. To date, structural information is available for the < /b> Zn(I...
Ngày tải lên: 07/03/2014, 09:20
Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen affinities of the a and b subunits potx
... (3) and < /b> (4), the < /b> dissociation rate con- stant, k, and < /b> the < /b> O 2 affinity, K , can be derived for both the < /b> a < /b> and < /b> b subunits from the < /b> averaged parameters of HbA oxygenation (Table 1, Average). The < /b> association and < /b> ... HbA in quite different ways. The < /b> decrease in the < /b> association rate constant of BR for the...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Cloning of a rat gene encoding the histo-blood group A enzyme Tissue expression of the gene and of the A and B antigens potx
... transferase) activity of the < /b> enzyme product and < /b> allowed detection of a < /b> small UDP-Gal:Fuca1,2GalaGal transferase (B transferase) activity. The < /b> presence of the < /b> mRNA and < /b> of the < /b> A < /b> and < /b> B antigens was searched ... analysis. Enzyme Species GenBank/EBI A < /b> transferase Human J05175 A < /b> transferase Rat AF264018 A < /b> tr...
Ngày tải lên: 31/03/2014, 09:20
Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... 5¢-GGTATTGAGGGTCGCCATGGTTATGTTC AATCACCA-3¢; reverse primer, 5¢-AGAGGAGAGTTAG AGCCTTACAAGAAGGGTCCAAAGA-3¢). The < /b> PCR product was purified, treated with T4 exonuclease to create vector-compatible overhangs and < /b> annealed ... LlCBP3 3A,< /b> the < /b> fast initial phase was maintained longer than in the < /b> absence of LlCBP3 3A,< /b> indicating that LlCBP3 3A < /b> acts synergi...
Ngày tải lên: 18/02/2014, 08:20
Báo cáo Y học: Substrate selectivity and sensitivity to inhibition by FK506 and cyclosporin A of calcineurin heterodimers composed of the a or b catalytic subunit potx
... isoform-specific antibodies confirm that CnAa and < /b> CnAb proteins are expressed by the < /b> appropriate recombin- ant CnAa and < /b> CnAb baculoviruses (Fig. 1B, C). Kinetic assays of CaNa and < /b> CaNb phosphatase activity Kinetic ... titered by plaque assay as described [10]. The < /b> coinfection, expression and < /b> purification of baculovirus-expressed CaN containing the <...
Ngày tải lên: 24/03/2014, 03:21
Báo cáo khoa học: Identification of the N-termini of NADPH : protochlorophyllide oxidoreductase A and B from barley etioplasts (Hordeum vulgare L.) ppt
... stromal processing peptidase (SPP) has been characterized, and < /b> a < /b> preferred consensus sequence for cleavage between a < /b> basic amino acid (arginine or lysine) and < /b> a < /b> C-terminal alanine was described ... Sequence alignment of pPORA and < /b> pPORB from barley and < /b> pPOR from pea. Arrowheads indicate the < /b> cleavage sites between the < /b> transit pe...
Ngày tải lên: 30/03/2014, 02:20
Báo cáo khoa học: Characterization of the 5¢ untranslated region of a and b isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms doc
... serving as a < /b> control. Thereafter, firefly and < /b> renilla luciferase activity was assayed and < /b> results are expressed as mean relative luciferase activity, in arbritary units (RLU) ± SEM(n ¼ 6). (D) As a < /b> complementary ... cells and < /b> HEK 293 cells. Thereafer, firefly and < /b> renilla luciferase activity was assayed and < /b> results are expressed as mean...
Ngày tải lên: 31/03/2014, 09:20