The a and b adapters are used as priming sites for both amplification
The a and b adapters are used as priming sites for both amplification
... B adapters are ligated to the < /b> blunt ends using DNA ligase• The < /b> strands are denatured using sodium hydroxide to release the < /b> ssDNA template library (sstDNA). The < /b> Adapters • The < /b> A < /b> and < /b> B adapters ... ssDNA bead to be loaded into a < /b> well.•Enzyme beads and < /b> packing beads are added. Enzyme beads containing sulfurase and < /b> luciferase, and < /b> packing beads used only to keep the < /b> DNA beads in place.•Above ... adapters are used as priming sites for both amplification and < /b> sequencing since their composition is known.• The < /b> B adapter contains a < /b> 5’ biotin tag used for mobilization.• The < /b> beads are magnetized...
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Báo cáo khoa học: Expressed as the sole Hsp90 of yeast, the a and b isoforms of human Hsp90 differ with regard to their capacities for activation of certain client proteins, whereas only Hsp90b generates sensitivity to the Hsp90 inhibitor radicicol pdf
... extracellular signal-regulated kinase-5 (ERK5)mitogen-activated protein (MAP) kinase [18].GR assays indicated that human Hsp9 0a < /b> and< /b> Hsp9 0b, as well as the < /b> native yeast Hsp90s, were allcapable ... mammalianclients are able to be activated by both Hsp9 0a < /b> and< /b> Hsp9 0b. Whether Hsp9 0a < /b> or Hsp9 0b is expressed in the < /b> yeast, however, has a < /b> dramatic effect on Hsp90inhibitor sensitivity. This raises ... theseHsp90s but 5¢ homologies to plasmid pBDC [34] (Hsp9 0a,< /b> forward primer GCTTGAAGCAAGCCTCGATGCCTGAGGAAACCCAGACCCAA, reverse primer CAGTAGCTTCATCTTTTCGGTCTACTTCTTCCATGCGTGA;Hsp9 0b, forward...
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Báo cáo khoa học: Coordination of three and four Cu(I) to the a- and b-domain of vertebrate Zn-metallothionein-1, respectively, induces significant structural changes doc
... and < /b> fourCu(I) loaded a-< /b> and < /b> b- domains, no interaction was seen between the < /b> twospecies. There was neither any indication for a < /b> net transfer of Cu(I)between the < /b> two domains nor for the < /b> formation ... vivo, and < /b> are believed to be important for d10-metalcontrol. To date, structural information is available for the < /b> Zn(II) and< /b> Cd(II) forms, but not for the < /b> Cu(I) or mixed metal forms. Cu(I) binding ... thisend, both Cu(I)-containing domains were prepared as for the < /b> titration experiment mentioned above, com-bined in equal amounts at a < /b> final concentration of1mm each and < /b> incubated for >48 h before...
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Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen affinities of the a and b subunits potx
... (3) and < /b> (4), the < /b> dissociation rate con-stant, k, and < /b> the < /b> O2affinity, K , can be derived for both the < /b> a < /b> and < /b> b subunits from the < /b> averaged parameters ofHbA oxygenation (Table 1, Average). The < /b> association and < /b> ... HbA inquite different ways. The < /b> decrease in the < /b> associationrate constant of BR for the < /b> a < /b> subunits is seen at a< /b> practically unchanged quantum yield of BR. By con-trast, the < /b> decrease in the < /b> association ... of BR for the < /b> a < /b> and < /b> b subunits within tri-liganded HbA.Time courses for O2rebinding are shown in Figs 1 and < /b> 2. The < /b> transient absorption decays were analyzedusing a < /b> standard least-squares...
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Báo cáo khoa học: Cloning of a rat gene encoding the histo-blood group A enzyme Tissue expression of the gene and of the A and B antigens potx
... transferase) activity of the < /b> enzyme product and < /b> alloweddetection of a < /b> small UDP-Gal:Fuca1,2GalaGal transferase (B transferase) activity. The < /b> presence of the < /b> mRNA and < /b> of the < /b> A < /b> and < /b> B antigens was searched ... analysis.Enzyme Species GenBank/EBI A < /b> transferase Human J05175 A < /b> transferase Rat AF264018 A < /b> transferase Pig AF050177 A(< /b> cis A/< /b> B) transferase Mouse AB041039 A-< /b> like a< /b> Human M65082Gal transferase ... sequences available in the < /b> NCBI database. The< /b> programs used are all available from http://www.infobio-gen.fr.Chromosome localization The < /b> Abo gene was first assigned to a < /b> rat chromosome using a < /b> panel...
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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... 5¢-GGTATTGAGGGTCGCCATGGTTATGTTCAATCACCA-3¢; reverse primer, 5¢-AGAGGAGAGTTAGAGCCTTACAAGAAGGGTCCAAAGA-3¢). The < /b> PCRproduct was purified, treated with T4 exonuclease tocreate vector-compatible overhangs and < /b> annealed ... LlCBP3 3A,< /b> the < /b> fastinitial phase was maintained longer than in the < /b> absenceof LlCBP3 3A,< /b> indicating that LlCBP3 3A < /b> acts synergis-tically with LlChi1 8A.< /b> However, the < /b> effect ofLlCBP3 3A < /b> was small and < /b> ... whereas enzymes withmore neutral pH optima have an aspartic acid at thisposition. For the < /b> latter type of enzyme, it has beenshown that mutation of aspartic acid to asparagineleads to a < /b> drastic...
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Báo cáo Y học: Substrate selectivity and sensitivity to inhibition by FK506 and cyclosporin A of calcineurin heterodimers composed of the a or b catalytic subunit potx
... isoform-specific antibodies confirm that CnAa and< /b> CnAb proteins are expressed by the < /b> appropriate recombin-ant CnAa and < /b> CnAb baculoviruses (Fig. 1B, C).Kinetic assays of CaNa and < /b> CaNb phosphatase activityKinetic ... titered by plaque assay as described [10]. The < /b> coinfection, expression and < /b> purificationof baculovirus-expressed CaN containing the < /b> rat brainCnAa subunit and < /b> rat brain CnB, or the < /b> human CnAbsubunit ... media, were obtained from Gibco/BRL. Fetal bovine serum was purchased from AtlantaBiologicals. CaM–Sepharose was purchased from Amer-sham Pharmacia. Antibiotics, FKBP12, and < /b> pNPP wereobtained...
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Báo cáo khoa học: Identification of the N-termini of NADPH : protochlorophyllide oxidoreductase A and B from barley etioplasts (Hordeum vulgare L.) ppt
... stromal processing peptidase (SPP)has been characterized, and < /b> a < /b> preferred consensussequence for cleavage between a < /b> basic amino acid(arginine or lysine) and < /b> a < /b> C-terminal alanine wasdescribed ... Sequence alignment of pPORA and < /b> pPORB from barley and < /b> pPOR from pea. Arrowheads indicate the < /b> cleavage sites between the< /b> transit peptide and < /b> the < /b> mature PORA protein. The < /b> transit peptide as described ... a < /b> branched pathway for light-dependent chlorophyll biosynthesis in Arabidopsisthaliana. Plant Physiol 108, 1505–1517.3 Oosawa N, Masuda T, Awai K, Fusada N, Shimada H,Ohta H & Takamiya...
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Báo cáo khoa học: Characterization of the 5¢ untranslated region of a and b isoforms of the human thromboxane A2 receptor (TP) Differential promoter utilization by the TP isoforms doc
... serving as a < /b> control. Thereafter, firefly and < /b> renilla luciferase activity was assayed and < /b> results are expressed as meanrelative luciferase activity, in arbritary units (RLU) ± SEM(n ¼ 6). (D) As a < /b> complementary ... cells and < /b> HEK 293 cells. Thereafer, firefly and < /b> renilla luciferase activity was assayed and < /b> results are expressed as mean relative luciferase activity, inarbritary units (RLU), ± SEM(n ¼ 6).Ó FEBS ... sites, the < /b> initial aim of this study was tocharacterize the < /b> major TP mRNA transcripts in HEL 92.1.7cells and < /b> in trophoblast TM-1 cells and,< /b> thereafter, toestablish the < /b> molecular basis of the...
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Từ khóa: blending with the a and b pdfthe recognition and manipulation of devices used to join sentences and form passages they are referred to as grammatical cohesion of a texta1 and a2 are used as aligners synchronizers they perform the same job as a preamble or flag field in other networks we cmonitored status in the vaccinated swine breeding herd is attained and maintained as outlined in parts a and b of this sectionincreased the accuracy of finding synonyms of our system for a set of k word is more accurate pn and test corpus are used for all our experiment evaluations that are two kinds of context vector context size evaluation and quality of systemphotograph showing the a first prototype of the scaffold and b the template mold used to contain the silk solutionthe discovery that micrornas mirnas are synthesized as hairpincontaining precursors and share many features has stimulated the development of several computational approaches for identifying new mirna genes in various animal specieshow can new technologies such as the internet and social media be used most effectivelytext s purpose students record the information to capture their thinking they use the recorded information to discuss and critique the accuracy and importance of information used to support a stance about the author s purposewhat is the value of a and b at end of this programmap the mental health system to understand the level of current resources and how they are used generally accepted accounting principles are a set of rules and practices that are recognized as a general guide for financial reporting purposespreamble parts 51 52 72 78 and 97 of chapter i of title 40 of the code of federal regulations are amended as followsrevolution about the x axis between a and beffect of goi land policies on the development prospects of areas a and bBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Nghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXQuản lý nợ xấu tại Agribank chi nhánh huyện Phù Yên, tỉnh Sơn La (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ