... RT-PCR analysis was performed as described previously. The expected sizes of the PCR products were 1270 bp for HVEM, 738 bp for nectin-1, 616 bp for nectin-2, and 736 bp for 3-OST-3, respectively. ... phalloidin (Invitrogen) was added for F-actin staining at room temperature for 45 min. Finally, the cells were washed three times with 1· NaCl ⁄ P i before micros- copy was performed...
Ngày tải lên: 18/02/2014, 14:20
... frames using PCR. For the first potential glycerol dehydrogenase gene, we predicted introns in the genomic DNA. For that rea- son, we amplified the open reading frame from cDNA. For the second of ... The tagged protein was purified and then used for further analysis. In the reductive reaction, the Michaelis–Menten con- stant K m for DHA was 1 mm, and the K m for NADPH was 50 lm. The...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Evidence for interactions between domains of TatA and TatB from mutagenesis of the TatABC subunits of the twin-arginine translocase docx
... indicated above the lanes) were assessed using export assays for TorA (upper panel) or TorA-GFP (lower panel) as described earlier for other mutants (Fig. 2). Mutagenesis of TatABC C. M. L. Barrett ... essential for Tat function. Taking these previ- ous reports into consideration, the logical conclusion is that several residues (F20, G21 in TatA, G21, P22, P26 in TatB) are probably impo...
Ngày tải lên: 19/02/2014, 17:20
Tài liệu Báo cáo khoa học: "Methods for the Qualitative Evaluation of Lexical Association Measures" doc
... for evaluation General statistics for the AdjN and PNV base sets are given in Table 1. Manual annotation was performed for AdjN pairs with frequency and PNV triples with only (see section 5 for ... best measure for identifying AdjN collocations, except for - coordinates between 15% and 20% where t-test outperforms log-likelihood. For PNV data, the curves of all measures (ex- cep...
Ngày tải lên: 20/02/2014, 18:20
Tài liệu Báo cáo khoa học: "Resolution for Machine Translation of Telegraphic Messages" docx
... most likely tag for each word is assigned, contextual transformations are used to improve the accuracy. We have evaluated the tagger performance on the TEST Data both before and after training ... originates from verbs, which provide information on subcategorization, and prepositions, which are critical for PP-attachment ambiguity res- olution. For the given domain, lexicalizing d...
Ngày tải lên: 22/02/2014, 03:20
Tài liệu Báo cáo khoa học: "PARAHETRIZED FOR LINGUISTIC ABSTRACT FORMATION OBJECTS PROCESSING" doc
... have adequate primitives for linguistic information processin~ and a clear semantics at the formal computational level are now slowly emergin~ as a convergent effort from computer science, ... choice of parametrized abstract objects as tools for linguistic information processing and exemplifies the use of such objects for temporal information. INTRODUCTION In computational l...
Ngày tải lên: 22/02/2014, 09:20
Báo cáo khoa học: "Methods for Using Textual Entailment in Open-Domain Question Answering" doc
... recognizing whether the information expressed in a text can be inferred from the information expressed in another text, can be performed in four ways. We can try to (1) derive linguistic information from ... another mechanism for approximating the types of inference needed in order answer questions accurately. In this paper, we present three different methods for incorporating systems fo...
Ngày tải lên: 08/03/2014, 02:21
Báo cáo khoa học: "Searching for Topics in a Large Collection of Texts" doc
... membership of a document in a concept-formative cluster is a function : . For we define where is a constant. For we define . The following holds true for any concept- -formative cluster and any document ... automatically discov- ered “concept-formative clusters” of documents. Shortly speaking, concept-formative clusters are semantically coherent and specific sets of docu- ments, which repr...
Ngày tải lên: 08/03/2014, 04:22
Báo cáo khoa học: "Project for production of closed-caption TV programs for the hearing impaired" docx
... processing methods for automatic text summarisation. The first is to compute key words in a text and importance measures for each sentence, and then select importanct sentences for the text. The ... Department of Information and Computer Science / TAO Abstract We describe an on-going project whose primary aim is to establish the technology of producing closed captions for TV...
Ngày tải lên: 08/03/2014, 06:20
Báo cáo khoa học: "Estimators for Stochastic "Unification-Based" Grammars*" docx
... well-formed analyses. Each feature f maps a syntactic anal- ysis w E ~ to a real value f(w). The form of a syntactic analysis depends on the underlying linguistic theory. For example, for ... parse tree, for a LFG w would be a tuple consisting of (at least) a c-structure, an f- structure and a mapping from c-structure nodes to f-structure elements, and for a Chomskyian trans...
Ngày tải lên: 08/03/2014, 06:20