Báo cáo khoa học: "deals with the interpretation of conceptual operations" docx

Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate speciﬁcity by replacement of Asn149 with Asp docx

... His145Asnmutations on the afﬁnity of metal bindingto arginase IITo further examine the effects of the His120ﬁAsnand His145ﬁAsn mutations on the interaction of the enzyme with manganese ions, ... addition to removal of the more weakly bound Mn2+, the lower afﬁnity for thatmore tightly bound to the protein may explain the absence of Mn2+from the EDTA-treated species of the His145Asn variant. ... stabilization of the substrate in the Michaelis–Menten complex [24]. Alsoin agreement with this, the Kmvalue was not alteredby the full activation step. Although an effect of the mutations...

Báo cáo khoa học: Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts docx

... refers to the c.p.m.value of the test reaction minus the c.p.m. value from the controlreaction (reaction terminated immediately after the addition of [14C]arginine). The average c.p.m. for the ... inhibitor of ﬂavoproteins including animalNOS), and found no inhibition of the activity at con-centrations of diphenylene iodonium up to 10 lm (datanot shown). Second, the products of the reaction ... dimension,chloroform ⁄ methanol ⁄ acetic acid (2 : 4 : 4). The plate was thenautoradiographed. The markers were visualized by ninhydrin stain-ing, and then marked as dashed lines on the autoradiogram. The origin...

Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf

... bc1sub-complex, but the presence of only one of these two subunits does not substitutefor the other. After the addition of ISP, the binding of the last subunit (i.e. Qcr10p) ﬁnally occurs. These ﬁnd-ings ... [12,29,32,41]. Of course, the possibility that other protein components of the respi-ratory chain, such as subunits of the cytochrome coxidase, or of the TIM23 machinery, or even of the metabolite ... alsounknown. Furthermore, as in the case of the biogene-sis of other multi-subunit complexes of the mitochon-drial respiratory chain, the assistance of speciﬁcchaperone proteins is also required. The...

Tài liệu Báo cáo khoa học: Looking for the ancestry of the heavy-chain subunits of heteromeric amino acid transporters rBAT and 4F2hc within the GH13 a-amylase family ppt

... hcHATs, the regions of similarity cover the sequence segments within the C-terminal extracellulardomain. The segments correspond, in fact, with some of the a-amylase family CSRs, namely the b-strandsb2, ... is the light subunit thatpossesses the amino acid transportation activity,although without interacting with the heavy subunit itis unable to reach the plasma membrane. Thus, the role of the ... b-strandsb2, b3, b4 and b8 of the (b ⁄ a)8barrel domain, and forrBAT also with the short stretch near the C-terminus of domain B [9,19]. From the sequence–structure point of view, the basic difference...

Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt

... illustrates the quality of the experimental data(Fig. 2B).Reconstruction of the overall shape of the PLDa2from the X-ray scattering data was achieved by the ab initio modeling program dammin [24]. The ... fromthese data. The binding event with the dissociationconstant in the range of 0.1 mm might be associated with calcium binding to the C2 domain. For the sepa-rately expressed C2 domain of PLDa ... shouldoccupy 16% of the molecular volume, and thereforeseems too large to represent the lid-like structure at the top of the left view in Fig. 3. We speculate that the loosely structured region on the opposite...

Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf

... 1.5 kb apart. We therefore analyzed the promoter function of the 1.5 kb 5¢-ﬂanking region of the HO-2 gene in the present study.YN-1 cells were transfected with each construct of the HO-1 and HO-2 ... degrada-tion in the population of hemoglobin-positive cells.To the best of our knowledge, there has been noreport on the promoter function of the human HO-2Fig. 7. Characterization of the human ... Takentogether, these results indicate that hypoxia reduces the expression levels of HO-2 mRNA and protein inﬁve out of six cell lines, with the exception of KG1myeloid cells.Hypoxia decreases the...

Tài liệu Báo cáo khoa học: "Parsing with generative models of predicate-argument structure" pptx

... probability of an argument, we count the number of times it occurs with lex-ical categoryand is the th argument of the lexicalfunctorin question, divided by the number of times the th argument of ... occurrences of the head noun. In particular, all instances of the noun and verb occurring together in the training data (with the same predicate-argument relation betweenthem, but not necessarily with the ... span and the same categoryare considered equivalent if they delay the evalua-tion of the probabilities of the same words and ifthey have the same number of lexical heads, and if the ﬁrst two...

Tài liệu Báo cáo khoa học: "INTEGRATING WITH WORD BOUNDARY IDENTIFICATION SENTENCE UNDERSTANDING" docx

... pecially the fallacious ones, when they block the primary task of discovering the inter-relationships among the var- ious constituents of a sentence, which essentially is the essence of the understanding ... the problem of over-generalization. Contrary to conventional wisdom, we do not view the task of word boundary identification as separated from the task of sentence understanding. Rather, the ... tested in the domain of analogy-making. There are four compo- pents in this architecture: the conceptual network (en- codes linguistic concepts), the workspace (the working area), the coderack...

Tài liệu Báo cáo khoa học: "Type-Driven Semantic Interpretation of f-Structures" pptx

... k, one of them being an appropriate argument for the other, then the structures are in- terpreted according to principle (B1) as the functor resp. argument of the interpretation of their unifi- ... semantic interpretation, which is the topic here, the semantic structures are the range of the a-projector which maps substruc- tures of the f-structure into corresponding substruc- tures of the ... satisfies the f-description, a description of the f-structure which is built up by instantiation of the annotations of the context-free rules and projected off the c-structure by the correspondence...

Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf

... from the different localization of these proteins in the cell. The molecular mechanisms underlying the transportprocesses of GPCRs from the ER to the cell surfacehave recently become the subject ... localization of these proteins in the cell rather thanfrom the possible role of the D2R basic domain in the mechanism of D1–D2receptor heterodimerization. However, we ﬁnd that the acidicepitope ... localized in the plasma mem-brane and inside the cell. In the case of the dopa-mine D2receptor mutants, the degree of membranelocalization depended on the number of mutated resi-dues in the ic3...

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