... columns (Amersham Bio-sciences ⁄ GE Healthcare, Piscataway, NJ, USA), superfineG-25 Sephadex (Pharmacia ⁄ Pfizer, Oakville, ON, Canada)and a stirred ultrafiltration cell (Amicon Bioseparations ⁄Millipore, ... partially metallateddomain species, supporting the model of a noncooper-ative metallation mechanism. Although a distinctionbetween partially metallated intermediates and thefully metallated ... mechanism are that the partially metallatedand metal-free species are stable intermediates, and thus may have a poten-tial role in the currently undefined function of metallothionein.Abbreviations a- rhMT...