... scanning mode at a scanning rate of 100 nmÆmin)1, a time constant of 1 s, a bandwidth of 2 nm and a resolution of 0.5 nm. Both far- and near-UV spectra were measured at 20 °C and finalspectra ... analysis of fluorescence data A novel, qualitative approach to the analysis of foldingdata, complementary to the conventional presentation and analysis of unfolding and refolding transition curves, ... native conditions both mutants, PrPW175 and PrPW198, adopt the same a- helical conformation as the wild-type protein, displaying the characteristic minimaat 209 and 220 nm and a maximum at...